The Effects of Benzoxasol Derivate Compounds in Breast Cancer Cells

Breast cancer today is the most frequent cancer among women, and the second most common cause of cancer deaths among women. The aim of this study was to synthesize a new benzoxazole derivative, scan it for anti-cancer potential by MTT test using different breast cancer cell lines, and examine its effects on NF-κB and apoptosis related proteins by the western blot method. A newly synthesized benzoxazole derived compound was applied to cancer cell lines and its cytotoxicity was measured quantitatively by MTT test. Later, the level of its effects on NF-κB and apoptosis related proteins were examined. The structure of the compound synthesized in our study (5-amino-2-(p-bromobenzyl) benzoxazoleand 5-[4-chlorobutanamido]-2-(p-methylphenyl) benzoxazole were proved by elemental analysis. In the assay of the proteins by western, when heterocyclic compounds were added to the MDA and MCF-7 cell line, there was no difference from the control group in Apaf-1 and BCL-2 levels, but a reduction was observed in caspase and Nfkβ levels compared with the control group. It is seen that this newly synthesized heterocyclic compound increases apoptosis by reducing the activation of Nfkβ, and in this way has shown an effect of inhibiting tumor growth in cancer treatment

P491, I Trionfi / Petrarca. Image 078

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Synthesis and antimicrobial activity of some 5-[2-(morpholin-4-yl)acetamido] and/or 5-[2-(4-substituted piperazin-1-yl)acetamido]-2-(p-substituted phenyl)benzoxazoles

In this study, a series of twelve novel 5-[2-(morpholin-4-yl)acetamido] and/or 5-[2-(4-substituted piperazine-1-yl)acetamido]-2-(p-substituted phenyl]benzoxazole derivatives have been synthesized and their structures were confirmed by IR, 1 H NMR, and mass spectral data. These compounds were prepared by reacting 5-(2-chloroacetamido)-2-(4-p-substituted-phenyl)benzoxazoles, which were obtained by using 5-amino-2-[p-substituted-phenyl]benzoxazoles with chloroacetyl chloride, in the presence of morpholine or 1-substituted piperazines. All synthesized compounds 3Ϫ14 were tested by using the method of twofold serial dilution technique for in vitro activities against certain strains of Grampositive, Gram-negative bacteria as well as the yeasts Candida albicans, Candida krusei, and Candida glabrata in comparison with standard drugs. Microbiological results showed that the newly synthesized compounds possessed a broad spectrum of activity, showing MIC values of 3.12Ϫ50 μg/mL against the Candida species