52,091 research outputs found
Efficiency of Xist-mediated silencing on autosomes is linked to chromosomal domain organisation
BACKGROUND: X chromosome inactivation, the mechanism used by mammals to equalise dosage of X-linked genes in XX females relative to XY males, is triggered by chromosome-wide localisation of a cis-acting non-coding RNA, Xist. The mechanism of Xist RNA spreading and Xist-dependent silencing is poorly understood. A large body of evidence indicates that silencing is more efficient on the X chromosome than on autosomes, leading to the idea that the X chromosome has acquired sequences that facilitate propagation of silencing. LINE-1 (L1) repeats are relatively enriched on the X chromosome and have been proposed as candidates for these sequences. To determine the requirements for efficient silencing we have analysed the relationship of chromosome features, including L1 repeats, and the extent of silencing in cell lines carrying inducible Xist transgenes located on one of three different autosomes. RESULTS: Our results show that the organisation of the chromosome into large gene-rich and L1-rich domains is a key determinant of silencing efficiency. Specifically genes located in large gene-rich domains with low L1 density are relatively resistant to Xist-mediated silencing whereas genes located in gene-poor domains with high L1 density are silenced more efficiently. These effects are observed shortly after induction of Xist RNA expression, suggesting that chromosomal domain organisation influences establishment rather than long-term maintenance of silencing. The X chromosome and some autosomes have only small gene-rich L1-depleted domains and we suggest that this could confer the capacity for relatively efficient chromosome-wide silencing. CONCLUSIONS: This study provides insight into the requirements for efficient Xist mediated silencing and specifically identifies organisation of the chromosome into gene-rich L1-depleted and gene-poor L1-dense domains as a major influence on the ability of Xist-mediated silencing to be propagated in a continuous manner in cis
Non-Magnetic Spinguides and Spin Transport in Semiconductors
We propose the idea of a "spinguide", i.e. the semiconductor channel which is
surrounded with walls from the diluted magnetic semiconductor (DMS) with the
giant Zeeman splitting which are transparent for electrons with the one spin
polarization only. These spinguides may serve as sources of a spin-polarized
current in non-magnetic conductors, ultrafast switches of a spin polarization
of an electric current and, long distances transmission facilities of a spin
polarization (transmission distances can exceed a spin-flip length). The
selective transparence of walls leads to new size effects in transport.Comment: 4 pages, 2 figure
Predicting outcome following colorectal cancer surgery using Colorectal Biochemical and Haematological Outcome Model (Colorectal BHOM)
Evidence for a diffusion-controlled mechanism for fluorescence blinking of colloidal quantum dots
Fluorescence blinking in nanocrystal quantum dots is known to exhibit power-law dynamics, and several different mechanisms have been proposed to explain this behavior. We have extended the measurement of quantum-dot blinking by characterizing fluctuations in the fluorescence of single dots over time scales from microseconds to seconds. The power spectral density of these fluctuations indicates a change in the power-law statistics that occurs at a time scale of several milliseconds, providing an important constraint on possible mechanisms for the blinking. In particular, the observations are consistent with the predictions of models wherein blinking is controlled by diffusion of the energies of electron or hole trap states
Semantic analysis of field sports video using a petri-net of audio-visual concepts
The most common approach to automatic summarisation and highlight detection in sports video is to train an automatic classifier to detect semantic highlights based on occurrences of low-level features such as action replays, excited commentators or changes in a scoreboard. We propose an alternative approach based on the detection of perception concepts (PCs) and the construction of Petri-Nets which can be used for both semantic description and event detection within sports videos. Low-level algorithms for the detection of perception concepts using visual, aural and motion characteristics are proposed, and a series of Petri-Nets composed of perception concepts is formally defined to describe video content. We call this a Perception Concept Network-Petri Net (PCN-PN) model. Using PCN-PNs, personalized high-level semantic descriptions of video highlights can be facilitated and queries on high-level semantics can be achieved. A particular strength of this framework is that we can easily build semantic detectors based on PCN-PNs to search within sports videos and locate interesting events. Experimental results based on recorded sports
video data across three types of sports games (soccer, basketball and rugby), and each from multiple broadcasters, are used to illustrate the potential of this framework
Fatty-acid uptake in prostate cancer cells using dynamic microfluidic raman technology
It is known that intake of dietary fatty acid (FA) is strongly correlated with prostate cancer progression but is highly dependent on the type of FAs. High levels of palmitic acid (PA) or arachidonic acid (AA) can stimulate the progression of cancer. In this study, a unique experimental set-up consisting of a Raman microscope, coupled with a commercial shear-flow microfluidic system is used to monitor fatty acid uptake by prostate cancer (PC-3) cells in real-time at the single cell level. Uptake of deuterated PA, deuterated AA, and the omega-3 fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were monitored using this new system, while complementary flow cytometry experiments using Nile red staining, were also conducted for the validation of the cellular lipid uptake. Using this novel experimental system, we show that DHA and EPA have inhibitory effects on the uptake of PA and AA by PC-3 cells
Comment on: Kinetic Roughening in Slow Combustion of Paper
We comment on a recent Letter by Maunuksela et al. [Phys. Rev. Lett. 79, 1515
(1997)].Comment: 1 page, 1 figure, http://polymer.bu.edu/~hmakse/Home.htm
Model-based monitoring and control of industrial freeze-drying processes: Effect of batch nonuniformity
The Use of Gamma-ray Bursts as Direction and Time Markers in SETI Strategies
When transmitting a signal over a large distance it is more efficient to send
a brief beamed signal than a continuous omni-directional transmission but this
requires that the receiver knows where and when to look for the transmission.
For SETI, the use of various natural phenomena has previously been suggested to
achieve the desired synchronization. Here it is proposed that gamma-ray bursts
may well the best ``synchronizers'' of all currently known phenomena due to
their large intrinsic luminosities, high occurrence rate, isotropic sky
distribution, large distance from the Galaxy, short duration, and easy
detectability. For targeted searches, precise positions for gamma-ray bursts
are required together with precise distance measurements to a target star. The
required burst position determinations are now starting to be obtained, aided
in large part by the discovery of optical afterglows. Good distance
measurements are currently available from Hipparcos and even better
measurements should be provided by spacecraft now being developed. For
non-targeted searches, positional accuracies simply better than a detector's
field of view may suffice but the time delay between the detection of a
gamma-ray burst and the reception of the transmitted signal cannot be predicted
in an obvious way.Comment: 8 pages, accepted for publication in PAS
Symmetry and designability for lattice protein models
Native protein folds often have a high degree of symmetry. We study the
relationship between the symmetries of native proteins, and their
designabilities -- how many different sequences encode a given native
structure. Using a two-dimensional lattice protein model based on
hydrophobicity, we find that those native structures that are encoded by the
largest number of different sequences have high symmetry. However only certain
symmetries are enhanced, e.g. x/y-mirror symmetry and rotation, while
others are suppressed. If it takes a large number of mutations to destabilize
the native state of a protein, then, by definition, the state is highly
designable. Hence, our findings imply that insensitivity to mutation implies
high symmetry. It appears that the relationship between designability and
symmetry results because protein substructures are also designable. Native
protein folds may therefore be symmetric because they are composed of repeated
designable substructures.Comment: 13 pages, 10 figure
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