50 research outputs found
Next-generation sequencing and its new possibilities in medicine
Next-Generation Sequencing (NGS) originally refers to high-throughput, massively parallel sequencing methods that allow the sequencing of up to billions of small (50-1000 bp), amplified DNA fragments at the same time but nowadays, there are NGS techniques that determine the sequence of long (up to 50 kbp) single molecules. Over the past years, NGS technologies become widely available with increasing throughput and decreasing sequencing costs per base making them more cost effective than the previously used capillary sequencing methods based on Sanger biochemistry. Nowadays, high-throughput DNA sequencing is routinely used on a wide range of important fields of biology and medicine enabling large-scale sequencing projects like analysis of complete genomes, disease association studies, whole transcriptomes, methylomes and provide new insights into complex biological systems. In addition, more and more NGS-based diagnostic tools are being introduced into the clinical practice, for example, on the fields of oncology, inherited and infectious diseases or pre-implantation and prenatal genetic screenings
BĂșza transzformĂĄciĂł | Wheat transformation
Az elmĂșlt Ă©vtizedben a növĂ©nyi biotechnolĂłgiĂĄban bekövetkezett mĂłdszertani fejlĆdĂ©s
eredmĂ©nyekĂ©nt lehetĆvĂ© vĂĄlt gyökeresen Ășj növĂ©nynemesĂtĂ©si programok tervezĂ©se Ă©s
elindĂtĂĄsa. A transzgĂ©nikus növĂ©nyek elĆĂĄllĂtĂĄsa komplex folyamat, ami magĂĄban foglalja az
idegen DNS molekulĂĄk bejuttatĂĄsĂĄt a sejtekbe, ezen nukleotid szekvenciĂĄk integrĂĄciĂłjĂĄt a
gazdasejt DNS-Ă©be Ă©s a bevitt gĂ©n követhetĆ kifejezĆdĂ©sĂ©t. A transzformĂĄciĂł mĂłdszere
lehetĆsĂ©get kĂnĂĄl többek között olyan gazdasĂĄgilag fontos gĂ©nek bevitelĂ©re a bĂșzanövĂ©nybe,
melyekkel növelhetĆ a rezisztencia biotikus Ă©s abiotikus stresszel szemben, a felhasznĂĄlĂłi
igĂ©nyeknek megfelelĆen vĂĄltoztathatĂł a tĂ©szta technolĂłgiai minĆsĂ©ge, javĂthatĂł a
bĂșzafehĂ©rjĂ©k tĂĄplĂĄlkozĂĄstani Ă©rtĂ©ke, tovĂĄbbĂĄ amelyekkel növelhetĆ a növĂ©nyekben a
vitaminok, vagy olyan mikroelemek mennyisége is, mint pl. a vas, vagy a cink. A szåmos
módszertani nehézség és a fogyasztói reakciók miatt, a növényi transzformåció módszerében
mĂ©g tovĂĄbbi jelentĆs fejlĆdĂ©s vĂĄrhatĂł a közeljövĆben. Ez a szemle a szerzĆk angol nyelven
megjelent összefoglalĂłjĂĄnak (Rakszegi et al. 2001) a legĂșjabb eredmĂ©nyekkel bĆvĂtett
vĂĄltozata.
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Plant biotechnology offers many new opportunities for breeders to solve
certain breeding problems at the molecular level. The tissue culture
methodology and the molecular genetic modification of economically
important monocotyledons have undergone a revolution in the last
decade. As the production of transgenic plants is a complex procedure
including the uptake of DNA molecules into the cells, the integration
of these nucleotide sequences into the host DNA and the expression of
new genes in a controlled way, and as there are still many unsolved
questions, further development is necessary. The methodology opens up
the possibility of introducing novel genes that may induce resistance
to diseases and abiotic stresses, allow the modification of dough
quality and the dietetic quality of proteins, and increase the levels
of micronutrients such as iron, zinc and vitamins. The present review
is an updated Hungarian version of the summary of the most important
advances in wheat transformation published by the authors in English
(Rakszegi et al. 2001)
In vitro micropropagation of 4 Hungarian bryophytes as potential sources for pharmacologically active substances
The plant kingdom is a rich source of pharmacologically active biomolecules, and
bryophytes represent a relatively unexploited taxon within plants. From this point
of view, Hungarian mosses are very promising, since only a small percent of the
659 species has been studied in detail. The aim of this project is to analyse
Hungarian bryophyte species as potential sources of molecules for anticancer drug
and antibiotic research. Important steps during the research are to identify
bryophyte species with bioactivity, to isolate and to identify their active
components and to characterize their effects in detail. Based on an in vitro
screening of 58 species, the most promising species are further studied. In order to
ensure sustainability of further research, in vitro micropropagation studies - of
species regarded as the most promising - are being carried out. Optimization of
surface sterilization (using 10% CaCl2O2 or 10% commercial Domestos) of
Brachythecium rutabulum, Oxyrrhynchium hians, Tortula muralis and Campylopus
introflexus is completed. In the selected bryophytes selection of appropriate
explants (shoot tips or lateral shoots), optimization of substrates (1/2 MS without
sucrose) for in vitro cultivation were carried out. Various explants of bryophyte
species, different surface sterilization methods, various culture media with
different plant hormone combinations and without hormones (1 mg l-1 NAA/ 1 mg
l
-1 BA/ 1 mg l-1 2, 4 D/ 1 mg l-1 BA+ 0.1 mg l-1 NAA/ 2 mg l-1 BA/ 1 mg l-1 NAA) were
tested for optimal growth and morphogenesis. New moss shoots or protonema
developments, depending on the hormonal supply, were observed. Growth
response was recorded in every selected species during in vitro cultivation.
The research was supported by the grant NKFI OTKA 115796 (supervisor DezsĆ
Csupor, âHungarian bryophytes as potential sources for pharmacologically active
substancesâ). Work of BoglĂĄrka TĂłth was supported by the ĂNKP-17-2 New
National Excellence Program of the Ministry of Human Capacities
MƱszaki-, agrĂĄr,- Ă©s informatikus diplomĂĄk Ă©rtĂ©ke a munkaerĆpiacon
Nowadays the rising unemployment rate for newly graduated students poses a considerable problem. Although obtaining a degree and a certificate is not enough on the labour market, it is a fact that college graduates' unemployment rates overall are still lower than the national average. In this paper we focus on the value of engineering and technology degrees, agricultural degrees and the informatics degree on the labour market.Napjainkban a pĂĄlyakezdĆk növekvĆ munkanĂ©lkĂŒlisĂ©gi rĂĄtĂĄja komoly problĂ©mĂĄt jelent. A diploma Ă©s bizonyĂtvĂĄny mĂĄr nem elegendĆ a munkaerĆpiacon, ugyanakkor tĂ©ny, hogy a munkanĂ©lkĂŒlisĂ©gi rĂĄta alacsonyabb a diplomĂĄsok korĂ©ben, mint a nemzetgazdasĂĄg ĂĄtlagos Ă©rtĂ©ke. ĂrĂĄsunk a mƱszaki,- agrĂĄr,- Ă©s informatikai diplomĂĄk munkaerĆpiaci Ă©rtĂ©kĂ©t mutatja be
MƱszaki-, agrĂĄr,- Ă©s informatikus diplomĂĄk Ă©rtĂ©ke a munkaerĆpiacon
Nowadays the rising unemployment rate for newly graduated students poses a considerable problem. Although obtaining a degree and a certificate is not enough on the labour market, it is a fact that college graduates' unemployment rates overall are still lower than the national average. In this paper we focus on the value of engineering and technology degrees, agricultural degrees and the informatics degree on the labour market.Napjainkban a pĂĄlyakezdĆk növekvĆ munkanĂ©lkĂŒlisĂ©gi rĂĄtĂĄja komoly problĂ©mĂĄt jelent. A diploma Ă©s bizonyĂtvĂĄny mĂĄr nem elegendĆ a munkaerĆpiacon, ugyanakkor tĂ©ny, hogy a munkanĂ©lkĂŒlisĂ©gi rĂĄta alacsonyabb a diplomĂĄsok korĂ©ben, mint a nemzetgazdasĂĄg ĂĄtlagos Ă©rtĂ©ke. ĂrĂĄsunk a mƱszaki,- agrĂĄr,- Ă©s informatikai diplomĂĄk munkaerĆpiaci Ă©rtĂ©kĂ©t mutatja be
MunkaerĆ - piaci kutatĂĄson alapulĂł kĂ©pzĂ©sinnovĂĄciĂł a Szolnoki FĆiskolĂĄn
A Szolnoki FĆiskola a TĂMOP-4.1.1/A-10/1/KONV-2010-19 azonosĂtĂłszĂĄmĂș âMunkaerĆ-piaci igĂ©nyekhez al-kalmazkodĂł hallgatĂłi Ă©s intĂ©zmĂ©nyi szolgĂĄltatĂĄsfejlesztĂ©s a Szolnoki FĆiskolĂĄn" EU pĂĄlyĂĄzat keretĂ©ben kiemelt cĂ©lkĂ©nt fogalmazta meg a munkaerĆ-piaci elvĂĄrĂĄsokhoz igazodĂł kĂ©pzĂ©si portfoliĂł kialakĂtĂĄsĂĄt, a kĂ©pzĂ©s fejlesztĂ©-sĂ©t Ă©s az oktatĂĄs gyakorlatorientĂĄciĂłjĂĄnak javĂtĂĄsĂĄt. Ennek megvalĂłsĂtĂĄsa Ă©rdekĂ©ben kiterjedt szekunder Ă©s pri-mer kutatĂĄst folytattunk, amelynek sorĂĄn lakossĂĄgi Ă©s szervezeti megkĂ©rdezĂ©s sorĂĄn tĂĄrtuk fel a vĂĄllalkozĂĄsok pĂĄlyakezdĆkkel szembeni elvĂĄrĂĄsait, annak szakmaspecifikus sajĂĄtossĂĄgait Ă©s a vĂĄrhatĂł keresletet. VĂ©gzett hall-gatĂłink megkĂ©rdezĂ©se a diploma munkaerĆ-piaci Ă©rtĂ©kĂ©nek Ă©s a tanulmĂĄnyok sorĂĄn megszerzett kompetenciĂĄk hasznosĂthatĂłsĂĄgĂĄnak feltĂĄrĂĄsĂĄra irĂĄnyult. KutatĂĄsunk eredmĂ©nyei a Szolnoki FĆiskola kĂ©pzĂ©sfejlesztĂ©sĂ©ben jĂłl hasznosĂthatĂłk, kiemelt tekintettel a tantervek struktĂșrĂĄjĂĄra, a tantĂĄrgyak tartalmĂĄra, az oktatĂĄs mĂłdszertanĂĄra Ă©s a szakmai gyakorlat rendszerĂ©re. E tanulmĂĄny bemutatja a többlĂ©pcsĆs primer kutatĂĄs eredmĂ©nyeit Ă©s annak hatĂĄsĂĄt a kĂ©pzĂ©s korszerƱsĂtĂ©sĂ©re, valamint a gyakorlati rendszer ĂĄtalakĂtĂĄsĂĄra. | College of Szolnok drew up the formation of a programme portfolio, its development and the improvement of educationâs practice-orientation aligning to labor market expectations as a priority within the framework of TĂMOP-4.1.1/A-10/1/KONV-2010-19 project âStudent and institutional service development aligned to labor force market needs in College of Szolnokâ. To achieve this goal we conducted an extended desk and field re-search, whereby we revealed during the consumer and organizational survey the expectations of companies against entrants, its profession-specific characteristics and the potential demand. Surveying our graduates aimed at revealing the labor force market value of the diploma and the expedience of competencies acquired during their studies. Results of our research can be well utilized in programme innovation of College of Szolnok, with special emphasis on the structure of syllabi, the methodology of education, the content of different subjects and the system of work placement. This study introduces the results of our staggered field research and its effect on programme modernization and transformation of work-placement system
In vitro micropropagation of 4 Hungarian bryophytes as potential sources for pharmacologically active substances
Ligand-Specific Regulation of the Endogenous Mu-Opioid Receptor by Chronic Treatment with Mu-Opioid Peptide Agonist
Since the discovery of the endomorphins (EM), the postulated endogenous peptide agonists of the mu-opioid receptors, several analogues have been synthesized to improve their binding and pharmacological profiles. We have shown previously that a new analogue, cis-1S,2R-aminocyclohexanecarboxylic acid2-endomorphin-2 (ACHC-EM2), had elevated mu-receptor affinity, selectivity, and proteolytic stability over the parent compound. In the present work, we have studied its antinociceptive effects and receptor regulatory processes. ACHC-EM2 displayed a somewhat higher (60%) acute antinociceptive response than the parent peptide, EM2 (45%), which peaked at 10âmin after intracerebroventricular (icv) administration in the rat tail-flick test. Analgesic tolerance developed to the antinociceptive effect of ACHC-EM2 upon its repeated icv injection that was complete by a 10-day treatment. This was accompanied by attenuated coupling of mu-sites to G-proteins in subcellular fractions of rat brain. Also, the density of mu-receptors was upregulated by about 40% in the light membrane fraction, with no detectable changes in surface binding. Distinct receptor regulatory processes were noted in subcellular fractions of rat brains made tolerant by the prototypic full mu-agonist peptide, DAMGO, and its chloromethyl ketone derivative, DAMCK. These results are discussed in light of the recently discovered phenomenon, that is, the âso-called biased agonismâ or âfunctional selectivity