Pengaruh Pemberian Bunga Rosella Terhadap Perubahan Tekanan Darah Penderita Hipertensi Dengan Terapi Captopril Di Desa Kamiwangi Kecamatan Toili Barat Kabupaten Luwuk Banggai

Hypertension is a cardiovasculer disturbance which is mostly founded in society. in healing hypertension, it needs continually Treatments. Rosella flower is a plant that has many good Components to heal hypertension. This research uses Quasy experiment design with control group. the amout of population in this research are 40 people. The method in taking sample uses purposive sampling and it got 30 respondents as the sample. The information of blood pressure was taken from the clients by using spygmomanometer, then it was displayed in table and processed by using SPSS program (statistic program for society science), used coupling T test, uncoupling T test with interpretation level 95%(a<0.05). The result of the research shows that the hypertension patients with captopril therapy which were given boiled dry rosella, got faster reduction of blood pressure after 2 hours than the hypertension patients with only captopril therapy. By using coupling T test, p-value was 0,00 . With mean value of the difference of reduction of sistolik blood pressure on the first day in intervention group was 28 mmHg while the control group was 11mmHg. on the second day, the reduction of sistolik blood pressure in intervention group was 13,33 l, and for control group, it was 5 mmHg. The diastolic blood pressure on the first day, the mean of the reduction of blood pressure in intervention group was 14mmHg while in control group was 6mmHg. On the second day, the mean of sistolic blood pressure in intervention group was 6 mmHg, and 2mmHg for control group. Key words: hypertension, rosella flower, captopril therapy Hipertensi merupakan penyakit gangguan kardiovaskuler. Bunga rosella adalah tumbuhan herbal untuk mengobati hipertensi. Tujuan penelitian melihat pengaruh bunga rosella terhadap tekanan darah penderita hipertensi dengan terapi captopril. Metode : quasi eksperiment design with control group, purposive sampling, 30 subyek (15 kelompok intervensi, 15 kelompok kontrol). Intervensi, subyek dengan captopril ditambahkan seduhan kering bunga rosella. Kontrol subyek mendapatkan dengan captopril saja. Sistolik pre kelompok intervensi, pada kategori hipertensi grade I, II, III. Sistolik post masuk pada kategori normal, pre hipertensi, hipertensi grade I, II, III. Diastolik pre kelompok intervensi terbesar kategori pre hipertensi, post diastolik terbesar berada pada kategori normal. Kelompok kontrol sistolik pre terbesar pada hipertensi grade II, post berada pada hipertensi grade I. Diastolik kelompok kontrol pre terbesar pada hipertensi grade I, post terbesar hipertensi grade I. Dianalisis dengan uji T berpasangan, dan uji T tidak berpasangan, α ≤ 0,05. Hasil Uji T berpasangan tekanan darah pre-post sistolik p-value 0,000, diastolik p-value 0,004, didapatkan pengaruh bunga rosella terhadap penderita hipertensi dengan captopril, penurunan tekanan darah kelompok intervensi sistolik 19,333, diastolik 10,000 mmHg, kelompok kontrol sistolik 9,000 mmHg, diastolik 4,333 mmHg. Hasil Uji T tidak berpasangan di dapatkan perbedaan penurunan tekanan darah kelompok intervensi lebih besar dibadingkan kelompok kontrol, dengan p-value, sistolik 0,000, diastolik 0,025. Perbedaan penurunan sistolik sebesar 12,333 mmHg, diastolik 6,333 mmHg. Kesimpulan didapatkan pengaruh pemberian bunga rosella terhadap Perubahan tekanan darah penderita hipertensi dengan terapi captopri

Nurr1 overexpression in NPs lines following lentivurs infection.

<p>Nurr1 mRNA levels detected by Real Time PCR in CTX, GE, SC, MB, adult SVZ and in ES-D3 derived NPs in control conditions and after infection with Nurr1 lentivirus. Nurr1 mRNA level in E12 mouse embryo MB (E MB) is shown as the positive control. The error bars represent standard deviation (n = 3). *** P<0,001.</p

Activation of the dopaminergic markers DAT and Ptx3 by Nurr1 overexpression.

<p>A) Representative samples of RT-PCR amplified products for DAT B) and C). real-time PCR of DAT (B) and Ptx3 (C) mRNA on differentiated cells in control conditions and after Nurr1 overexpression HPRT was used as normalizing gene. The error bars represent standard deviation (n = 3). ** P<0,02.</p

Evaluation of neuronal and glial cells in differentiated NPs lines.

<p>A) Immunostaining for beta-III-tubulin (green) GFAP (red) on differentiated cells in control conditions (A, C, E, G, I.M) and after infection with Nurr1 lentivirus (B, D, F, H, L, N). (A, B) CTX, (C, D) GE, (E, F) SC, (G, H) MB, (I, L) adult SVZ, (M, N) ES-D3. Nuclei were stained with DAPI. <b>B</b>) Percentage of beta-III-tubulin and GFAP positive cells after differentiation of NPs. The reported values are the mean ± SEM of 10 observations from two independent experiments Scale bar  = 20 μm. The error bars represent standard deviation.</p

Induction of TH expression by Nurr1 overexpression: immunocytochemistry analysis.

<p>A) Immunostaining for TH (green) on differentiated cells in control conditions and after infection with Nurr1 lentivirus. Nuclei were stained with DAPI (blue). (a) ES-D3, (d) Nurr1 infected ES-D3, (b) MB, (e) MB Nurr1 infected (c) GE, (f) Nurr1 infected GE. B) Percentage of TH positive cells (vs total cell number). Scale bar  = 30 μm.</p

Induction of TH expression by Nurr1 overexpression: RT-PCR analysis.

<p>TH mRNA level detected by Real Time PCR in differentiated CTX, GE, MB and ES-D3 NPs and in cells infected with Nurr1. TH mRNA was not detectable in other analyzed NPs. TH mRNA level in mouse MB (E MB) is reported as positive control. The error bars represent standard deviation (n = 3). *** P<0,001.</p

Immunodetection of Nestin in NPs lines derived from different brain regions and RT-PCR detection of regionally expressed genes.

<p>A) Immunostaining for nestin (green) on NPs derived from a) cortex (CTX), b) lateral ganglionic eminence (GE), c) spinal cord (SC), d) midbrain (MB), e) subventricular zone (SVZ), f) mouse blastocyst (ES-D3). Nuclei were stained with DAPI (blue). Scale bar  = 20 μm. B). B) Real time PCR quantification of Ngn2, HoxB6, HoxB9, Dlx2, Six3 and FoxG1 expression in CTX-, GE- and SC-derived NPs cultured in proliferating conditions. Results are shown as the mean of the ratio between gene expression in NPs and in dissected E13.5 tissue (telencephalon or spinal cord) in four biological replicates. Error bars show the standard error of the mean. * P<0,05 ***p<0.001.</p

Supplementary dataset 5

SUPPORTING DATA FOR FIG. S5

Mir-23a and mir-125b regulate neural stem/progenitor cell proliferation by targeting Musashi1

<div><p>Musashi1 is an RNA binding protein that controls the neural cell fate, being involved in maintaining neural progenitors in their proliferative state. In particular, its downregulation is needed for triggering early neural differentiation programs. In this study, we profiled microRNA expression during the transition from neural progenitors to differentiated astrocytes and underscored 2 upregulated microRNAs, miR-23a and miR-125b, that sinergically act to restrain Musashi1 expression, thus creating a regulatory module controlling neural progenitor proliferation.</p></div