747 research outputs found

    Adaptation and differentiation across island populations of Berthelot’s pipit (Anthus berthelotii)

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    The aim of this thesis was to examine how neutral and adaptive processes shape patterns of genetic diversity across populations of Berthelot’s pipit (Anthus berthelotii), a passerine bird endemic to the Canary Islands, Selvagens and Madeira archipelagos. To achieve this, I examined variation in pathogen infection, neutral microsatellites and functional major histocompatibility complex (MHC) genes. I first reviewed previous evidence for pathogen-mediated selection at the MHC, and showed that differentiating between specific mechanisms of balancing selection may not be possible, and that many studies that have attempted to do so have not fully considered alternative explanations. In Berthelot’s pipit, I found marked differences in prevalence of avian malaria and pox across populations, and showed that these differences were stable over time, largely because they were determined by biogeographic features. This, combined with an observed effect on host body condition, suggests that populations face differential selection pressures from pathogens. Microsatellite analyses indicated that the pipit colonised northwards across its range, resulting in genetic bottlenecks in the Selvagens and Madeira archipelagos. I then used the pipit system to assess how population genetic analyses were influenced by microsatellite markers with different levels of variability; lower variability loci appear to more accurately reflect population divergence, whereas higher variability loci better reflect past changes in population size. I also found that two commonly used measures of differentiation (GST and Jost’s D) are both strongly affected by marker variability, but in different ways. Finally, I found that just 11-15 MHC variants persisted through the initial colonisation event. However, since the bottleneck, at least 26 functional MHC alleles have been generated in situ across the different populations, all but two by gene conversion. Taken together, my results provide an interesting example of how founder events, mutation, drift and selection can interact to drive differentiation across natural populations

    454 screening of individual MHC variation in an endemic island passerine

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    Genes of the major histocompatibility complex (MHC) code for receptors that are central to the adaptive immune response of vertebrates. These genes are therefore important genetic markers with which to study adaptive genetic variation in the wild. Next-generation sequencing (NGS) has increasingly been used in the last decade to genotype the MHC. However, NGS methods are highly prone to sequencing errors, and although several methodologies have been proposed to deal with this, until recently there have been no standard guidelines for the validation of putative MHC alleles. In this study, we used the 454 NGS platform to screen MHC class I exon 3 variation in a population of the island endemic Berthelot’s pipit (Anthus berthelotii). We were able to characterise MHC genotypes across 309 individuals with high levels of repeatability. We were also able to determine alleles that had low amplification efficiencies, whose identification within individuals may thus be less reliable. At the population level we found lower levels of MHC diversity in Berthelot’s pipit than in its widespread continental sister species the tawny pipit (Anthus campestris), and observed trans-species polymorphism. Using the sequence data, we identified signatures of gene conversion and evidence of maintenance of functionally divergent alleles in Berthelot’s pipit. We also detected positive selection at 10 codons. The present study therefore shows that we have an efficient method for screening individual MHC variation across large datasets in Berthelot’s pipit, and provides data that can be used in future studies investigating spatio-temporal patterns and scales of selection on the MHC

    Deaf and Hard of Hearing Readers and Science Comics: A Mixed Methods Investigation on Process

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    Deaf and hard of hearing (DHH) students bring diverse language and literacy backgrounds to the task of academic reading, which becomes increasingly complex and abstract in the upper grades. Teachers often differentiate their instruction by providing multimedia resources, of which students interact with verbal and pictorial information. A growing body of research supports multimedia learning; however, most of the studies have focused exclusively on learning outcomes, leaving teachers in the dark about the cognitive processes underlying these effects. This mixed methods study addresses this gap by using a nonfiction comic to investigate the reading processes of DHH 7th -12th grade students. Eye tracking and cued retrospective protocol were employed in a concurrent nested design to answer the question, how do DHH students read and learn from multimedia science texts? This study was guided by the cognitive theory of multimedia which states that reading comprehension is better supported when learning from words and pictures rather than words alone, especially when readers cognitively integrate the two representations to form a coherent mental model. Temporal and sequential eye tracking results revealed that readers’ transitions between related words and pictures were a statistically significant variable in explaining factual knowledge learning outcomes. These strategic shifts in attention were further explained by readers’ retrospective verbal reports of their thinking. Students’ descriptions of their vii reading processes were interpreted into the following themes: repairing, connecting representations, passive transitions, and connecting to self. The integration of quantitative and qualitative methods at the interpretation stage revealed that although the theme of repairing was equally distributed across all student reports, the theme of connecting representations was largely present in the reports from students who made high counts of integrative transitions. The major findings of this study align with the cognitive theory of multimedia, that students’ learning outcomes were significantly predicted by the deliberate strategies to cognitively integrate words and pictures to form and maintain a coherent mental model. The discussion includes ways in which teachers can capitalize on explicit modeling of these behaviors and employ students’ “think alouds” to better understand and support the development of effective multimedia reading processes

    ARE AFRICAN AMERICAN ATHLETES AND CELEBRITIES OBLIGATED NOT TO USE THE N-WORD?

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    In 2014, the leadership of the National Football League instructed the league\u27s game officials to penalize players who use the n-word on the field. The league\u27s action sparked another installment of the long-running public debate over whether African Americans should use the n-word. The parties to the debate often adopt contrasting positions on whether African American athletes and celebrities are obligated morally not to use the term. This paper examines the most significant arguments, revealed by the public debate, in favor of such an obligation. By demonstrating that all of those arguments fail, I conclude that unless there is a sound argument for the obligation that I have overlooked, African American athletes and celebrities have no obligation that prohibits them from using the n-word

    Why the Duty to Self-Censor Requires Social-Media Users to Maintain Their Own Privacy

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    Revelations of personal matters often have negative consequences for social-media users. These consequences trigger frequent warnings, practical rather than moral in nature, that social-media users should consider carefully what they reveal about themselves since their revelations might cause them various difficulties in the future. I set aside such practical considerations and argue that social-media users have a moral obligation to maintain their own privacy that is rooted in the duty to self-censor. Although Anita L. Allen provides a paternalist justification of the duty that supports my position that social-media users are obligated to self-censor what they reveal about themselves, I justify the obligation through considerations that are more palatable to liberals than is paternalism. I accomplish this by arguing that the failure to self-censor often creates for others undue burdens that individuals are obligated morally not to create. In particular, social-media revelations often create undue burdens for those, such as employers and university personnel, who are obligated morally to respect individuals’ privacy in their decision-making processes. I also demonstrate that this argument is not for a broad duty to self-censor, but, rather, for a narrow duty that applies to particular circumstances such as certain uses of social media

    Occupational Safety and Paternalism: Machan Revisited

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    In 1987, Machan provided a libertarian case against the right to occupational safety. Since before Machan’s essay appeared, many business ethicists and legal scholars have given considerable attention to the overall position Machan endorses: the acceptance of employment at will and the rejection of employee rights. No one yet has given adequate attention, however, to the fact that Machan’s argument against the right to occupational safety actually stands or falls independently of his overall position on employee rights. His argument ultimately rests on two values: the promotion of employee interests and anti-paternalism. Insofar as those who support the right to occupational safety share those values, they must find a strategy for opposing Machan’s argument that preserves those values. In this paper, I demonstrate why Machan’s argument ultimately rests on the promotion of employee interests and anti-paternalism. Then, I develop an objection to Machan’s argument that preserves those values

    What\u27s so special about a special ethics for business?

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    In business ethics literature, debate over a special ethics generally has framed examination of the rules governing business. By constructing a dilemma faced by proponents of a special ethics, I argue that this framing is misguided. Proponents must adopt either an insular or a derivative conception. The former, the view that business is insulated from moral rules, is problematic because arguments used to support it force proponents to accept the idea that each aspect of life is insulated from moral rules. This idea, however, renders philosophically insignificant the claim that business has a special ethics. Proponents no longer make a claim about business, but, rather, a relativistic claim about ethics in general. The derivative conception is the view that business is a set of circumstances that bear on the application of moral rules. This, however, is true of each aspect of life, and is simply an application of the principle ‘ought implies can’. The result is that there is nothing special about this sense of a special ethics. Despite lacking specialness, however, the derivative conception provides proper framing for examination of the rules governing business. It subjects business to moral rules, but, also, accounts for the challenging circumstances businesspersons face
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