Technological development of hydroalcoholic extractive solutions from Calendula officinalis L. flowers by using factorial design

L., pelo processo de maceração (MAC) e de turbo-extração (TURB), empregando planejamento fatorial 32 (dois fatores e três níveis), para avaliar a influência do tempo de extração (MAC = 5, 10 e 15 dias; TURB = 5, 15 e 25 min) e da relação planta:solvente (2,5, 7,5 e 12,5%, m/V) sobre o teor de flavonóides totais e resíduo seco nas soluções extrativas. Para o processo de maceração, a relação planta: solvente de 12,5% e o tempo de 15 dias resultaram nas melhores condições para alcançar um maior teor de flavonóides totais, em relação à turbo-extração que obteve o mesmo desempenho em 5 min. Para ambos os processos, o resíduo seco foi proporcional à relação planta:solvente.The work aimed at the development of extractive solutions from the flowers of Calendula officinalis, through maceration (MAC) and turbo-extraction (TURB) processes, employing the 32 (two factors and three levels) factorial design, in order to evaluate the influence of the time (MAC = 5, 10 and 15 days; TURB = 5, 15 and 25 min) and of the plant: solvent ratio (2.5, 7.5 and 12.5%, w/v) on the flavonoid content and the dry residue yields in the extractive solutions. For the maceration process, the 12.5% (w/v) plant: solvent ratio over 15 days were the best conditions to achieve the highest flavonoid content, in the turbo-extraction process the same performance was achieved in 5 min. For both processes the dry residue was proportional to the plant: solvent ratio.Colegio de Farmacéuticos de la Provincia de Buenos Aire

A Model of DENV-3 Infection That Recapitulates Severe Disease and Highlights the Importance of IFN-γ in Host Resistance to Infection

There are few animal models of dengue infection, especially in immunocompetent mice. Here, we describe alterations found in adult immunocompetent mice inoculated with an adapted Dengue virus (DENV-3) strain. Infection of mice with the adapted DENV-3 caused inoculum-dependent lethality that was preceded by several hematological and biochemical changes and increased virus dissemination, features consistent with severe disease manifestation in humans. IFN-γ expression increased after DENV-3 infection of WT mice and this was preceded by increase in expression of IL-12 and IL-18. In DENV-3-inoculated IFN-γ−/− mice, there was enhanced lethality, which was preceded by severe disease manifestation and virus replication. Lack of IFN-γ production was associated with diminished NO-synthase 2 (NOS2) expression and higher susceptibility of NOS2−/− mice to DENV-3 infection. Therefore, mechanisms of protection to DENV-3 infection rely on IFN-γ-NOS2-NO-dependent control of viral replication and of disease severity, a pathway showed to be relevant for resistance to DENV infection in other experimental and clinical settings. Thus, the model of DENV-3 infection in immunocompetent mice described here represents a significant advance in animal models of severe dengue disease and may provide an important tool to the elucidation of immunopathogenesis of disease and of protective mechanisms associated with infection

CHEMICAL CHANGES IN BEAN GRAINS DURING STORAGE IN CONTROLLED CONDITIONS

Banjarmasin, Borneo (Kalimantan, Indonesia) 195-GrayscaleForman Safety Negatives, Box

Characterization of Dengue Virus Type 2: New Insights on the 2010 Brazilian Epidemic

Dengue viruses (DENV) serotypes 1, 2, and 3 have been causing yearly outbreaks in Brazil. In this study, we report the re-introduction of DENV2 in the coast of São Paulo State. Partial envelope viral genes were sequenced from eighteen patients with dengue fever during the 2010 epidemic. Phylogenetic analysis showed this strain belongs to the American/Asian genotype and was closely related to the virus that circulated in Rio de Janeiro in 2007 and 2008. The phylogeny also showed no clustering by clinical presentation, suggesting that the disease severity could not be explained by distinct variants or genotypes. The time of the most recent common ancestor of American/Asian genotype and the São Paulo and Rio de Janeiro (SP/RJ) monophyletic cluster was estimated to be around 40 and 10 years, respectively. Since this virus was first identified in Brazil in 2007, we suggest that it was already circulating in the country before causing the first documented outbreak. This is the first description of the 2010 outbreak in the State of São Paulo, Brazil, and should contribute to efforts to control and monitor the spread of DENVs in endemic areas

Systematic analysis of jellyfish galaxy candidates in Fornax, Antlia, and Hydra from the S-PLUS survey: A self-supervised visual identification aid

© 2024 The Author(s). Published by Oxford University Press on behalf of Royal Astronomical Society. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/We study 51 jellyfish galaxy candidates in the Fornax, Antlia, and Hydra clusters. These candidates are identified using the JClass scheme based on the visual classification of wide-field, twelve-band optical images obtained from the Southern Photometric Local Universe Survey. A comprehensive astrophysical analysis of the jellyfish (JClass > 0), non-jellyfish (JClass = 0), and independently organized control samples is undertaken. We develop a semi-automated pipeline using self-supervised learning and similarity search to detect jellyfish galaxies. The proposed framework is designed to assist visual classifiers by providing more reliable JClasses for galaxies. We find that jellyfish candidates exhibit a lower Gini coefficient, higher entropy, and a lower 2D Sérsic index as the jellyfish features in these galaxies become more pronounced. Jellyfish candidates show elevated star formation rates (including contributions from the main body and tails) by 1.75 dex, suggesting a significant increase in the SFR caused by the ram-pressure stripping phenomenon. Galaxies in the Antlia and Fornax clusters preferentially fall towards the cluster's centre, whereas only a mild preference is observed for Hydra galaxies. Our self-supervised pipeline, applied in visually challenging cases, offers two main advantages: it reduces human visual biases and scales effectively for large data sets. This versatile framework promises substantial enhancements in morphology studies for future galaxy image surveys.Peer reviewe

Screening, production and biochemical characterization of a new fibrinolytic enzyme produced by Streptomyces sp. (Streptomycetaceae) isolated from Amazonian lichens

Thrombosis is a pathophysiological disorder caused by accumulation of fibrin in the blood. Fibrinolytic proteases with potent thrombolytic activity have been produced by diverse microbial sources. Considering the microbial biodiversity of the Amazon region, this study aimed at the screening, production and biochemical characterization of a fibrinolytic enzyme produced by Streptomyces sp. isolated from Amazonian lichens. The strain Streptomyces DPUA1576 showed the highest fibrinolytic activity, which was 283 mm2. Three variables at two levels were used to assess their effects on the fibrinolytic production. The parameters studied were agitation (0.28 - 1.12 g), temperature (28 - 36 ºC) and pH (6.0 - 8.0); all of them had significant effects on the fibrinolytic production. The maximum fibrinolytic activity (304 mm2) was observed at 1.12 g, 28 ºC, and pH of 8.0. The crude extract of the fermentation broth was used to assess the biochemical properties of the enzyme. Protease and fibrinolytic activities were stable during 6 h, at a pH ranging from 6.8 to 8.4 and 5.8 to 9.2, respectively. Optimum temperature for protease activity ranged between 35 and 55 °C, while the highest fibrinolytic activity was observed at 45 ºC. Proteolytic activity was inhibited by Cu2+ and Co2+ ions, phenylmethylsulfonyl fluoride (PMSF) and pepstatin A, which suggests that the enzyme is a serine protease. Enzymatic extract cleaved fibrinogen at the subunits A-chain, A-chain, and -chain. The results indicated that Streptomyces sp. DPUA 1576 produces enzymes with fibrinolytic and fibrinogenolytic activity, enzymes with an important application in the pharmaceutical industry.The authors grateful acknowledge the financial support of Fundação de Amparo a Pesquisa do Estado de Pernambuco (FACEPE, Pernambuco, Brazil, N. 0158-2.12/11), CNPq/ RENORBIO (National Counsel of Technological and Scientific Development, N.55146/2010-3) and National Council for the Improvement of Higher Education (CAPES, Brazil) for the scholarship. The author thanks editor and reviewers for their review and comments.info:eu-repo/semantics/publishedVersio