10 research outputs found
A physiological and biochemical approach to understanding cell dormancy mechanism of unicellular eukaryote <i>Colpoda</i><b> </b>
No full textTolerance of Colpoda cucullus Nag-1 wet resting cysts to extreme pH (pH 1 and 13): Implications of less permeability of the cyst membrane to H+ and OH
No full textExcystment-Dependent Alteration of Protein Expression in Terrestrial Ciliate Colpoda cucullus
No full textCulture Age, Intracellular Ca2+ Concentration, and Protein Phosphorylation in Encystment-Induced Colpoda cucullus
No full textLepidosomes acquire fluorescence after encystation: Including additional notes of morphological events during encystation and reconsideration of the morphological features in the ciliate Colpoda cucullus
No full textComparison of the morphology and viability of gamma irradiated vegetative cells, wet cysts, and dry cysts of the soil ciliate Colpoda cucullus
No full textFig. 7 in Analysis of Water-Soluble Proteins by Two-Dimensional Electrophoresis in the Encystment Process of Colpoda cucullus Nag-1 and Cytoskeletal Dynamics
No full textFig. 7. Effects of 10 µM taxol (A) and 10 µM cytochalasin B (B) on Ca2+/overpopulation-mediated globulation of C. cucullus Nag-1 (A-1, B-1) and ciliary resorption (A-2, B-2). A-1, B-1 − The rate of encysting (rounded) cells was expressed as a percentage of the total number of tested cells (100 randomly selected cells). Open squares (negative control). The cells were suspended in 1 mM Tris-HCl (pH 7.2) solu- tion without inhibitors at low cell density ( 30,000 cells/ml) (Ca2+/overpopulation stimulation). In this condition, the encystment was markedly induced. Open circles (experiment). The cells were suspended in an encystment-inducing medium containing taxol (Ta) or cytochalasin B (CB) at high cell density (> 30,000 cells/ml). Points and attached bars correspond to the means of 5 measurements (100 cells per measurement) obtained from different batches and standard errors, respectively. A-2, B-2 − Length of cilia at 2 h after onset of encystment induction in the presence or absence of taxol (Ta) or cytochalasin B (CB). In the negative control [Induced without 'Ta' (0 h) or Induced without 'CB' (0 h)], the cultured cells were collected, then suspended in encystment-inducing medium, and quickly fixed with 3.7% paraformaldehyde. In the positive control [Induced without 'Ta' (2 h) or Induced without 'CB' (2 h)], the cells were suspended for 2 h in an encystment-inducing medium without inhibitors at high cell density (> 30,000 cells/ml), and then fixed with 3.7% paraformaldehyde. In the experimental groups [Induced with 'Ta' (2 h) or Induced with 'CB' (2 h)], the cells were suspended for 2 h in an encystment-inducing medium containing inhibitors at high cell density (> 30,000 cells/ml), and then fixed with 3.7% paraformaldehyde. Columns and attached bars correspond to the means in 26 cells and standard errors, respectively.Published as part of Sogame, Yoichiro, Kojima, Katsuhiko, Takeshita, Toshikazu, Kikuchi, Shiho, Shimada, Yuto, Nakamura, Rikiya, Arikawa, Mikihiko, Miyata, Seiji, Kinoshita, Eiji, Suizu, Futoshi & Matsuoka, Tatsuomi, 2020, Analysis of Water-Soluble Proteins by Two-Dimensional Electrophoresis in the Encystment Process of Colpoda cucullus Nag-1 and Cytoskeletal Dynamics, pp. 107-120 in Acta Protozoologica 59 on page 117, DOI: 10.4467/16890027AP.20.009.13264, http://zenodo.org/record/835695
Analysis of Water-Soluble Proteins by Two-Dimensional Electrophoresis in the Encystment Process of Colpoda cucullus Nag-1 and Cytoskeletal Dynamics
Get PDFSogame, Yoichiro, Kojima, Katsuhiko, Takeshita, Toshikazu, Kikuchi, Shiho, Shimada, Yuto, Nakamura, Rikiya, Arikawa, Mikihiko, Miyata, Seiji, Kinoshita, Eiji, Suizu, Futoshi, Matsuoka, Tatsuomi (2020): Analysis of Water-Soluble Proteins by Two-Dimensional Electrophoresis in the Encystment Process of Colpoda cucullus Nag-1 and Cytoskeletal Dynamics. Acta Protozoologica 59: 107-120, DOI: 10.4467/16890027AP.20.009.13264, URL: http://dx.doi.org/10.4467/16890027ap.20.009.1326
