Fig. 7 in Analysis of Water-Soluble Proteins by Two-Dimensional Electrophoresis in the Encystment Process of Colpoda cucullus Nag-1 and Cytoskeletal Dynamics

Abstract

Fig. 7. Effects of 10 µM taxol (A) and 10 µM cytochalasin B (B) on Ca2+/overpopulation-mediated globulation of C. cucullus Nag-1 (A-1, B-1) and ciliary resorption (A-2, B-2). A-1, B-1 − The rate of encysting (rounded) cells was expressed as a percentage of the total number of tested cells (100 randomly selected cells). Open squares (negative control). The cells were suspended in 1 mM Tris-HCl (pH 7.2) solu- tion without inhibitors at low cell density ( 30,000 cells/ml) (Ca2+/overpopulation stimulation). In this condition, the encystment was markedly induced. Open circles (experiment). The cells were suspended in an encystment-inducing medium containing taxol (Ta) or cytochalasin B (CB) at high cell density (> 30,000 cells/ml). Points and attached bars correspond to the means of 5 measurements (100 cells per measurement) obtained from different batches and standard errors, respectively. A-2, B-2 − Length of cilia at 2 h after onset of encystment induction in the presence or absence of taxol (Ta) or cytochalasin B (CB). In the negative control [Induced without 'Ta' (0 h) or Induced without 'CB' (0 h)], the cultured cells were collected, then suspended in encystment-inducing medium, and quickly fixed with 3.7% paraformaldehyde. In the positive control [Induced without 'Ta' (2 h) or Induced without 'CB' (2 h)], the cells were suspended for 2 h in an encystment-inducing medium without inhibitors at high cell density (> 30,000 cells/ml), and then fixed with 3.7% paraformaldehyde. In the experimental groups [Induced with 'Ta' (2 h) or Induced with 'CB' (2 h)], the cells were suspended for 2 h in an encystment-inducing medium containing inhibitors at high cell density (> 30,000 cells/ml), and then fixed with 3.7% paraformaldehyde. Columns and attached bars correspond to the means in 26 cells and standard errors, respectively.Published as part of Sogame, Yoichiro, Kojima, Katsuhiko, Takeshita, Toshikazu, Kikuchi, Shiho, Shimada, Yuto, Nakamura, Rikiya, Arikawa, Mikihiko, Miyata, Seiji, Kinoshita, Eiji, Suizu, Futoshi & Matsuoka, Tatsuomi, 2020, Analysis of Water-Soluble Proteins by Two-Dimensional Electrophoresis in the Encystment Process of Colpoda cucullus Nag-1 and Cytoskeletal Dynamics, pp. 107-120 in Acta Protozoologica 59 on page 117, DOI: 10.4467/16890027AP.20.009.13264, http://zenodo.org/record/835695