Analysis Concentration of Toxoplasma gondii on Anti-Toxoplasma IgG-IgM Antibody Levels, and the Outcomes of Pregnancy in Mice Balb/c

Introduction: Toxoplasma gondii is an obligate intracellular protozoan para-site that can infect any warm blood vertebrae, and if first trimester pregnant woman infected, it may cause abortion. The objective is to prove the effect of the Toxoplasma gondii concentration in anti-toxoplasma IgG-IgM antibody levels, and the outcomes of Balb/c mice pregnancies. Materials and Methods: The study was conducted in Balb/c mice with inclusion criteria, and was con-ditioned pregnant. The pathogen strains of Toxoplasma gondii tachyzoite in-jected intraperitoneally. The blood samples were taken serially to be tested for anti-toxoplasma IgG-IgM antibody levels. After the mice were injected with tachyzoite, they are assessed every day to observe their body weight, vaginal bleeding, and labor. Anti-toxoplasma IgG-IgM antibody levels examined us-ing qualitative mouse IgG-IgM antibody ELISA KIT. Results: Anti-toxoplasma IgM antibody levels increased significantly after 24 hours of injection tachy-zoites in all dose groups, and remained high through day 21. Anti-toxoplasma antibody IgG levels increased significantly after 72 hours post injection and remained elevated until day 21. The incidence of abortion is 100% in mice which injected tachyzoite levels 1 × 103 and 1 × 104, and the incidence of abor-tion approximately 2 - 4 days post injection. 100% of mice that were injected with tachyzoites 1 × 101 and 1 × 102 have labor at term. Physical anomaly was found in baby mice from mice that were injected with tachyzoite 1 × 102. Conclusion: There is a significant correlation between the concentrations of Toxoplasma gondii tachyzoite with anti-toxoplasma IgG-IgM antibody levels

Ocimum Basilicum as Alternative Natural Cancer Care

Cancer is the most dreadful disease worldwide in terms of morbidity and mortality. The exact cause of cancer development and progression is not fully known. Ocimum Basilicum (OB) or basil contains citric acid, essential oils, 1-8 sineol, arigin, anetol, flavonoid, boron, stigmasterol, eugenol, beta-carotene, magnesium and tryptophan. Works as an anticancer through phenolic compounds (caffeine acid, p-kumarat acid) which is an antiproliferative agent, which will increase the number of non-apoptotic cells in the synthesis phase in the cell cycle and decrease the number of non-apoptotic cells in the G2/M phase. While caffeine acid may cause an increase in FasL, which is a receptor of death (Fas). The existence of a complex between FasL and Fas can induce signaling that induces cell death. Phenolics have also been shown to decrease the Bcl2 antiapoptotic protein, so apoptosis does not work. Increased Bcl2 expression will be followed by an increase in Bax, which is produced because cancer cells may mutate in the p53 protein, triggering a dimer between the two and triggering apoptosis through Fas. OB extract, containing flavonoids that have an effect as a chemopreventive agent by protecting lymphocytes from cancer induction. Another compound is the essential oil (terpenoid) that can inhibit tumor by improving the performance of Human Natural Killer in immune system (immunostimulan), so as to destroy tumor cells and increase macrophage cells that act as the phagocytes of damaged cells. In this review will be discussed about the role of OB in the natural treatment of cancer and the working mechanisms involved in it

Animal Modeling Try Strain Balb/c Mice With Gardnerella Vaginalis

Gardnerella vaginalis is the causative agent of sexually transmitted infections (STIs) of the bacterial class and is the main species of bacterial vaginosis (BV), classified as gram-negative bacteria, and is anaerobic facultative. The incidence of bacterial vaginosis increased rapidly in Indonesia with a prevalence of approximately 20-30% of women of childbearing age suffering from sexually transmitted infections diagnosed BV and an increase of 50-60%. BV is the leading cause of vaginal infection in women of childbearing age and is one of the most common infectious diseases in the gynecological obstetrics. Balb-C is a trying animal with a human-like cervical vagina. Objective: To identify the dose of Gardnerella vaginalis bacteria that has the potential to live on the vagina of experimental animals. Methods: Balb/c female strain, adult age 8-12 weeks, weight 20-25 g. Using the patient's urine specimen suspect Bacterial vaginosis was inoculated on intravaginal Balb-c each of 10 ?l doses of 3 x 106, 3 x 105, and 3 x 104. Prior to inoculation (H0), a vaginal swab was taken and continued at H

The Role of FSH, LH, Estradiol and Progesterone Hormone on Estrus Cycle of Female Rats

The estrous cycle is a recurrent process that describes changes in reproductive hormone levels caused by ovarian activity under the influence of pituitary hormones. Estrous cycles are characterized by morphological changes in ovaries, the uterus and the vaginal cells. The length of the estrous cycle and the frequency of the reproductive cycle in each organism are different. In mice, lasts for about 4-5 days. The estrus cycle consists of four phases, namely proestrus, estrus, metestrus and diestrus. During this period, the vaginal mucosa undergoes tremendous structural change

Postpartum Anxiety and Stress during the Covid-19 Pandemic

This study aims to obtain information about the anxiety and stress of postpartum mothers during the COVID-19 pandemic. This study used a descriptive survey method on a sample of pregnant women and women giving birth by purposive sampling, namely those who visited the Independent Practice Midwife (BPM) clinic in Bekasi City from April to July 2020, totaling 78 people. Data were collected using the DASS-21 questionnaire which was packaged in a google form and distributed online via WhatsApp social media. The data collected was then analyzed descriptively to be presented as a result of the research. The results showed that the psychological response to anxiety was normal 50 (64.10%), mild 14 (17.95%), moderate 11 (14.10%), severe 2 (2.56%), and very heavy 1 (1.28 %) of respondents. While the psychological response to normal stress was 59 (75.64%), mild 11 (14.10%), moderate 6 (7.69%), and severe 2 (2.56%) experienced psychologically normal but some experienced anxiety and stress. Most of the psychological responses to normal anxiety, but there are mild, moderate, severe, and very severe anxiety, as well as psychological responses to stress, although most normal, there is also mild, moderate, and severe stress. &nbsp