Identification of the growth hormone-releasing hormone analogue [Pro1, Val14]-hGHRH with an incomplete C-term amidation in a confiscated product

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Stability of epitaxial heterostructured materials

Heterostructured materials are a new family of artificial compounds where the electronic and ionic properties can be modulated by varying the characteristics of the different material layers. These properties arise from the formation of structural oxygen defects in the crystal lattice that result in the activation of charge electrical carriers. Oxygen-deficient perovskite oxides, such as La1-xSrxCoO3-δ (LSC), present mixed oxide/electronic conduction; however, the long-term instability due to superficial carbonation of LSC-based cathodes is a crucial drawback for their practical application. In this study, thin film-heterostructures of alternating layers of La0.6Sr0.4CoO3-δ and Ce0.8Gd0.2O2-δ (CGO) were deposited on (110) NdGaO3 (NGO) single crystal substrates by pulsed laser deposition (PLD). The number of interfaces and the thickness were varied to obtain epitaxial structures with highly coherence layers. Moreover, two different kinds of architectures, without and with a CGO termination layer, were prepared in order to study the stability of the samples under different thermal cycles in air. Structural characterization was made by using Rocking Curve and Reciprocal Space Mapping techniques. CGO layers are rotated 45º respect to the substrate and LSC ones due to the different sizes of cell parameters. The quality of the samples was examined by HR-TEM and all of them presented well defined interfaces (Figure 1). Electrical characterization confirms that the conductivity can be modulated by varying the number of interfaces and thickness. Samples without CGO termination are unstable in air atmosphere due to surface carbonation, which was confirmed by XPS and HR-TEM.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Poaceae vs. Abiotic stress: Focus on drought and salt stress, recent insights and perspectives

Poaceae represent the most important group of crops susceptible to abiotic stress. This large family of monocotyledonous plants, commonly known as grasses, counts several important cultivated species, namely wheat (Triticum aestivum), rice (Oryza sativa), maize (Zea mays), and barley (Hordeum vulgare). These crops, notably, show different behaviors under abiotic stress conditions: wheat and rice are considered sensitive, showing serious yield reduction upon water scarcity and soil salinity, while barley presents a natural drought and salt tolerance. During the green revolution (1940–1960), cereal breeding was very successful in developing high-yield crops varieties; however, these cultivars were maximized for highest yield under optimal conditions, and did not present suitable traits for tolerance under unfavorable conditions. The improvement of crop abiotic stress tolerance requires a deep knowledge of the phenomena underlying tolerance, to devise novel approaches and decipher the key components of agricultural production systems. Approaches to improve food production combining both enhanced water use efficiency (WUE) and acceptable yields are critical to create a sustainable agriculture in the future. This paper analyzes the latest results on abiotic stress tolerance in Poaceae. In particular, the focus will be directed toward various aspects of water deprivation and salinity response efficiency in Poaceae. Aspects related to cell wall metabolism will be covered, given the importance of the plant cell wall in sensing environmental constraints and in mediating a response; the role of silicon (Si), an important element for monocots' normal growth and development, will also be discussed, since it activates a broad-spectrum response to different exogenous stresses. Perspectives valorizing studies on landraces conclude the survey, as they help identify key traits for breeding purposes

Mechanism(s) of action of heavy metals to investigate the regulation of plastidic glucose-6-phosphate dehydrogenase

The regulation of recombinant plastidic glucose-6P dehydrogenase from Populus trichocarpa (PtP2-G6PDH - EC 1.1.1.49) was investigated by exposing wild type and mutagenized isoforms to heavy metals. Nickel and Cadmium caused a marked decrease in PtP2-G6PDH WT activity, suggesting their poisoning effect on plant enzymes; Lead (Pb++) was substantially ineffective. Copper (Cu++) and Zinc (Zn++) exposition resulted in strongest decrease in enzyme activity, thus suggesting a physiological competition with Magnesium, a well-known activator of G6PDH activity. Kinetic analyses confirmed a competitive inhibition by Copper, and a mixed inhibition by (Cd++). Mutagenized enzymes were differently affected by HMs: the reduction of disulfide (C175-C183) exposed the NADP+ binding sites to metals; C145 participates to NADP+ cofactor binding; C194 and C242 are proposed to play a role in the regulation of NADP+/NADPH binding. Copper (and possibly Zinc) is able to occupy competitively Magnesium (Mg++) sites and/or bind to NADP+, resulting in a reduced access of NADP+ sites on the enzyme. Hence, heavy metals could be used to describe specific roles of cysteine residues present in the primary protein sequence; these results are discussed to define the biochemical mechanism(s) of inhibition of plant plastidic G6PDH