Additional file 1: Figure S1. of Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin

Sequence of B. subtilis subsp. subtilis str. 168 genome sequence [Genbank:CP010052.1] corresponding to the region containing part of Contig00019 and Contig00022. There is a gap of 37 basepairs (uncoloured region in the figure) between Contig00019 (pink region in figure) and Contig00022 (yellow region in figure), when aligning the obtained contigs to B. subtilis subsp. subtilis str. 168 genome sequence [Genbank:CP010052.1]. PCR and sequence analysis were used to confirm that the genomic regions present on both contigs are indeed adjacent in the GM-Bacillus genome. Hereto, primers Scaf-19-F3-seq (positioned on Contig00019, indicated in green) and Scaf-22-R-seq (positioned on Contig00022, indicated in blue) were used to amplify the flanking regions of the junction between Contig00019 and Contig00022. Subsequently, the obtained PCR fragment was sequenced. (PDF 101 kb

Satory, 4/2/25, concours d'endurance de véhicules pour l'armée, chenille Citroën 10 HP : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 98415Appartient à l’ensemble documentaire : Pho20RolImage de press

Additional file 1: Table S1. of The genetic structure of the Belgian population

SNP variant allele frequencies of the Belgian population, BelPHG-21 study, Belgium 2016. Variant allele frequencies (VAF) for all SNPs were calculated with reference to the human genome build hg19 on all samples or after exclusion of samples with a foreign origin (indicated by tab names). Columns indicate SNP ids, chromosome, position, reference allele, variant allele, VAF, and frequencies of homozygous reference (AA), heterozygous (AB), and homozygous variant (BB) alleles respectively. (XLSX 28534 kb

Additional file 6: Figure S4. of The genetic structure of the Belgian population

Variant allele frequency correlations between Belgian and other populations. Variant allele frequencies (VAF) for all SNPs were calculated with reference to the human genome build hg19. Plots show the VAF of Belgian versus other continental (a) and European (b) populations. Pearson correlation coefficients are shown on top of each plot. For visualization purposes, only 1000 random points are shown. (PDF 5259 kb

Additional file 2: Table S2. of The genetic structure of the Belgian population

Population details of the samples analyzed in the BelPHG-21 study, Belgium 2016. Indication of the number of samples taken from NHIS-2013 participants in each region and province, total number of inhabitants in 2013, number of samples taken per million inhabitants, number of invited participants, and sampling percentages. (XLSX 10 kb