Differentially expressed transcripts of Tetracapsuloides bryosalmonae (Cnidaria) between carrier and dead‑end hosts involved in key biological processes: novel insights from a coupled approach of FACS and RNA sequencing

Tetracapsuloides bryosalmonae is a malacosporean endoparasite that infects a wide range of salmonids and causes proliferative kidney disease (PKD). Brown trout serves as a carrier host whereas rainbow trout represents a dead-end host. We thus asked if the parasite adapts to the different hosts by changing molecular mechanisms. We used fluorescent activated cell sorting (FACS) to isolate parasites from the kidney of brown trout and rainbow trout following experimental infection with T. bryosalmonae. The sorted parasite cells were then subjected to RNA sequencing. By this approach, we identified 1120 parasite transcripts that were expressed differentially in parasites derived from brown trout and rainbow trout. We found elevated levels of transcripts related to cytoskeleton organisation, cell polarity, peptidyl-serine phosphorylation in parasites sorted from brown trout. In contrast, transcripts related to translation, ribonucleoprotein complex biogenesis and subunit organisation, non-membrane bounded organelle assembly, regulation of protein catabolic process and protein refolding were upregulated in rainbow trout-derived parasites. These findings show distinct molecular adaptations of parasites, which may underlie their distinct outcomes in the two hosts. Moreover, the identification of these differentially expressed transcripts may enable the identification of novel drug targets that may be exploited as treatment against T. bryosalmonae. We here also describe for the first time how FACS based isolation of T. bryosalmonae cells from infected kidney of fish fosters research and allows to define differentially expressed parasite transcripts in carrier and dead-end fish hosts

Brief note on the infestation of pedunculate barnacles in crabs

An investigation was made to isolate and identify parasites from Charybdis lucifera landed at Cochin Fisheries Harbour in January, 2018. The carapace, branchial chambers and gills of each crab was observed for the presence of octolasmids. Octolasmis spp. are generally known as stalked barnacles or pedunculate barnacles. They are common in shallow waters and often found attached to the exoskeleton of the decapod crustacea, including crabs. This can affect the marketing of such infected commercially important crabs

Brief note on infestation of Diplectanum sp. in Asian seabass

Recently open sea cage farming has emerged as an alternative and additional income source for fishermen and fish farmers in India. Asian seabass, Lates calcarifer is widely used in open sea cage culture due to its high market demand. In cage farming, high stocking densities and poor water quality enhance the parasite loads of the cultured fishes. In this study, 47 specimens of Asian seabass collected from cages located in Naganathwada, Sunkeri, Ankola were analysed with the aim of identifying the parasites prevalent among this species. All external and internal organs of each fish were examined separately under microscope for parasites. The collected monogenean parasites were washed in a 0.85% saline solution and fixed in 70% ethanol and identified. Most of the infected fishes had dark coloration of the body and postmortem findings revealed gills with excessive mucus secretion and sticking of the gill tips with greyish coloration

Report on Amyloodinium spp. cysts infection in clownfish

A study was undertaken to record the occurrence of parasitic infections in ocellaris clownfish, Amphiprion ocellaris. Of a total eight A. ocellaris maintained in hatchery, three were found infected with different developmental stages of Amyloodinium spp. and were kept under observation

Development of Fish Parasite Vaccines in the OMICs Era: Progress and Opportunities

Globally, parasites are increasingly being recognized as catastrophic agents in both aquaculture sector and in the wild aquatic habitats leading to an estimated annual loss between 1.05 billion and 9.58 billion USD. The currently available therapeutic and control measures are accompanied by many limitations. Hence, vaccines are recommended as the “only green and effective solution” to address these concerns and protect fish from pathogens. However, vaccine development warrants a better understanding of host–parasite interaction and parasite biology. Currently, only one commercial parasite vaccine is available against the ectoparasite sea lice. Additionally, only a few trials have reported potential vaccine candidates against endoparasites. Transcriptome, genome, and proteomic data at present are available only for a limited number of aquatic parasites. Omics-based interventions can be significant in the identification of suitable vaccine candidates, finally leading to the development of multivalent vaccines for significant protection against parasitic infections in fish. The present review highlights the progress in the immunobiology of pathogenic parasites and the prospects of vaccine development. Finally, an approach for developing a multivalent vaccine for parasitic diseases is presented. Data sources to prepare this review included Pubmed, google scholar, official reports, and websites

Identification of in vivo induced antigens of the malacosporean parasite Tetracapsuloides bryosalmonae (Cnidaria) using in vivo induced antigen technology

Tetracapsuloides bryosalmonae is a malacosporean endoparasite that causes proliferative kidney disease (PKD) in wild and farmed salmonids in Europe and North America. The life cycle of T. bryosalmonae completes between invertebrate bryozoan and vertebrate fish hosts. Inside the fish, virulence factors of T. bryosalmonae are induced during infection or interactions with host cells. T. bryosalmonae genes expressed in vivo are likely to be important in fish pathogenesis. Herein, we identify in vivo induced antigens of T. bryosalmonae during infection in brown trout (Salmo trutta) using in vivo induced antigen technology (IVIAT). Brown trout were exposed to the spores of T. bryosalmonae and were sampled at different time points. The pooled sera were first pre-adsorbed with antigens to remove false positive results. Subsequently, adsorbed sera were used to screen a T. bryosalmonae cDNA phage expression library. Immunoscreening analysis revealed 136 immunogenic T. bryosalmonae proteins induced in brown trout during parasite development. They are involved in signal transduction, transport, metabolism, ion-protein binding, protein folding, and also include hypothetical proteins, of so far unknown functions. The identified in vivo induced antigens will be useful in the understanding of T. bryosalmonae pathogenesis during infection in susceptible hosts. Some of the antigens found may have significant implications for the discovery of candidate molecules for the development of potential therapies and preventive measures against T. bryosalmonae in salmonids.publishedVersio

Proteomic approaches and their applications in salmonid health research

Salmonid aquaculture is the fastest growing and important food production industry. However, salmonid aquaculture is often plagued with disease incidences that cause huge economic losses. Globally, many research groups are actively working to understand the diseases of salmonids and develop sustainable remedial measures. Proteomics is an important approach to decipher the molecular processes involved during host–pathogen interaction. For the past 2 decades, the advancement of high-throughput proteomic technologies has enhanced the understanding of protein dynamics in various organisms under different conditions. Similarly, the proteomic approaches could be efficiently used to understand the infectious diseases in salmonids. In this chapter, we have discussed various proteomic techniques and their applications. Moreover, in the latter part of this chapter, special focus is shed upon the proteomic approaches employed to understand enteric redmouth and myxozoan parasitic diseases of salmonids. We suggest that the proteomic approach can help to understand the disease dynamics and generate proteomic profiles of the pathogens of salmonids. Subsequently, this information can aid in the development of effective prophylactic measures against salmonid diseases