Additional file 7: of Isocitrate dehydrogenase 1–snail axis dysfunction significantly correlates with breast cancer prognosis and regulates cell invasion ability

Figure S3. IDH1 stable knockdown significantly promoted MDA-MB-231 cell motility. (a) The expression levels of IDH1 were examined in two IDH1 stable knockdown MDA-MB-231 cells (shIDH1#1 and shIDH1#2) through western blotting. (b) Invasion ability was assessed using the Transwell assay in MDA-MB-231 cells with IDH1 stable knockdown and a scrambled control. The cell images of the representative experiment are provided. (c) Values were quantified using Ascent software, as detailed. Data are reported as the number of invading cells relative to the control (means Âą standard deviation (SD)). (d) Expression levels of IDH1, snail, slug, twist, and actin were examined in shIDH1#1, shIDH1#2, and the scrambled control through western blotting. (TIFF 3572 kb

Additional file 9: of Isocitrate dehydrogenase 1–snail axis dysfunction significantly correlates with breast cancer prognosis and regulates cell invasion ability

Figure S5. IDH1 knockdown accelerated MCF7 cell proliferation and migration ability. (a) the expression levels of IDH1 were examined in MCF7 cells with siIDH1#1, siIDH1#2, and control transfection through western blotting. (b) A wound healing assay was employed to examine MCF7 cells transfected with siIDH1#1, siIDH1#2, and the scrambled control. (c) The expression levels of IDH1, snail, slug, twist and actin were examined in siIDH1#1, siIDH1#2, and the scrambled control through western blotting. (d) The proliferation assay was performed in MCF-7 cells transfected with the scrambled control, siIDH1#1 and siIDH1#2, respectively. (TIFF 4527 kb

Additional file 1: of Isocitrate dehydrogenase 1–snail axis dysfunction significantly correlates with breast cancer prognosis and regulates cell invasion ability

Table S1. Primer sequence list. (DOC 38 kb

Additional file 10: of Isocitrate dehydrogenase 1–snail axis dysfunction significantly correlates with breast cancer prognosis and regulates cell invasion ability

Figure S6. Gene set enrichment analysis for differential expression of genes in MDA-MB-231 cells transfected with si-IDH1 compared with those transfected with scramble control. (a) Differential expression of genes (upregulated or downregulated twofold change) was identified using a microarray approach. These gene sets were significantly enriched in metastasis-associated terms from the Kyoto Encyclopedia of Genes and Genomes. (b) Schematic putative signaling pathway illustrating IDH1-modulated cancer cell invasion. (TIFF 1728 kb

Additional file 8: of Isocitrate dehydrogenase 1–snail axis dysfunction significantly correlates with breast cancer prognosis and regulates cell invasion ability

Figure S4. IDH1 knockdown significantly accelerated HS578T and BT549 cell motility. (a), (d) Invasion ability was assessed using the Transwell assay in HS578T and BT549 cells with si-IDH1 and scramble control. The cell images of a representative experiment are shown. (b), (e) Values quantified using Ascent software are shown. Data are reported as the number of colonies relative to the control (means Âą standard deviation (SD)). (c), (f) Expression levels of EMT-related markers were examined in HS578T and BT549 cells transfected with si-IDH1 and scramble control by western blotting. (TIFF 4403 kb

Additional file 3: of Isocitrate dehydrogenase 1–snail axis dysfunction significantly correlates with breast cancer prognosis and regulates cell invasion ability

Table S3. Correlation of IDH1 expression with molecular markers in patients with breast cancer. (DOCX 16 kb