Additional file 8: of Isocitrate dehydrogenase 1âsnail axis dysfunction significantly correlates with breast cancer prognosis and regulates cell invasion ability
Figure S4. IDH1 knockdown significantly accelerated HS578T and BT549 cell motility. (a), (d) Invasion ability was assessed using the Transwell assay in HS578T and BT549 cells with si-IDH1 and scramble control. The cell images of a representative experiment are shown. (b), (e) Values quantified using Ascent software are shown. Data are reported as the number of colonies relative to the control (means Âą standard deviation (SD)). (c), (f) Expression levels of EMT-related markers were examined in HS578T and BT549 cells transfected with si-IDH1 and scramble control by western blotting. (TIFF 4403 kb
