2 research outputs found
Identification of a Cardiolipin-specific Phospholipase Encoded by the Gene CLD1 (YGR110W) in Yeast*
The mitochondrial dimeric phospholipid cardiolipin is characterized by a
high degree of unsaturation of its acyl chains, which is important for its
functional interaction with mitochondrial enzymes. The unusual fatty acid
composition of cardiolipin molecular species emerges from a de novo
synthesized “premature” species by extensive acyl chain remodeling
that involves as yet only partially identified acyltransferases and
phospholipases. Recently, the yeast protein Taz1p was shown to function as a
transacylase, which catalyzes the reacylation of monolysocardiolipin to mature
cardiolipin. A defect in the orthologous human TAZ gene is associated
with Barth syndrome, a severe genetic disorder, which may lead to cardiac
failure and death in childhood. We now identified the protein encoded by
reading frame YGR110W as a mitochondrial phospholipase, which
deacylates de novo synthesized cardiolipin. Ygr110wp has a strong
substrate preference for palmitic acid residues and functions upstream of
Taz1p, to generate monolysocardiolipin for Taz1p-dependent reacylation with
unsaturated fatty acids. We therefore rename the Ygr110wp as Cld1p
(cardiolipin-specific deacylase 1)