UJI EFEK ANTIINFLAMASI EKSTRAK ETANOL BIJI LAMTORO (Leucaena leucocephala L.) TERHADAP UDEM TELAPAK KAKI MENCIT YANG DIINDUKSI KARAGENIN

Telah dilakukan penelitian mengenai uji aktivitas antiinflamasi ekstrak etanol biji lamtoro (Leucaena leucocephala L.) pada mencit (Mus musculus). Lamtoro telah dikenal oleh masyarakat Indonesia secara turun-temurun dapat mengobati bengkak. Penelitian ini dilakukan untuk mengetahui apakah ekstrak etanol biji lamtoro dapat berkhasiat sebagai antiinflamasi serta mengetahui dosis optimum ekstrak biji lamtoro yang dapat berkhasiat sebagai antiinflamasi. Uji aktivitas antiinflamasi dilakukan dengan metode pembentukan radang buatan pada telapak kaki mencit putih jantan. Dilakukan penelitian dengan menggunakan 15 ekor mencit yang dibagi menjadi 5 kelompok perlakuan dengan kriteria berumur 2-3 bulan dengan berat 20-30 g. Pengujian dilakukan menggunakan 3 dosis yang berbeda dari ekstrak biji lamtoro yaitu 200 mg/20 g BB, 400 mg/20 g BB dan 600 mg/20 g BB. Berdasarkan penelitian di dapatkan persen radang terbesar berada pada dosis 200 mg/20 g BB dan persen radang terkecil pada dosis 600 mg/20 g BB. Data hasil pengukuran besar radang diuji statistik dengan metode LSD dengan hasil kelompok dosis 200 mg/20 g BB tidak memiliki potensi khasiat sebagai antiinflamasi dan kelompok dosis 400 mg/20 g BB serta dosis 600 mg/20 g BB mempunyai potensi khasiat sebagai antiinflamasi

HUBUNGAN PELAYANAN INFORMASI OBAT TERHADAP KEPATUHAN MINUM OBAT PASIEN HIPERTENSI DI RSUD PENAJAM PASER UTARA

The Drug Information Service or Pelayanan Informasi Obat (PIO) is indispensable for improving patient compliance with their treatment. With the PIO can change the knowledge and compliance of patients, especially hypertensive patients. This study was conducted to determine whether PIO can influence adherence to medication hypertension patients in RSUD Penajam Paser Utara. Sampling was done by purposive sampling and analyzed using Spearman Rank Correlation Test where the compliance aspect was assessed using validity and reliability tested questionnaire method, given before PIO and after PIO, and Pill Count method (calculating the remaining pills for 4 weeks) . Samples were obtained by 50 people, the results showed no improvement in adherence before and after PIO administration, and there was a relationship of compliance measurement results with questionnaire method after counseling and Pill Count method. The percentage of patient adherence after PIO administration was based on a high-compliance 70% (35 person) questionnaire method and based on the Pill Count method with a high adherence rate of 60% (30 persons), the compliance data obtained from both methods indicated that the provision of PIO can not improve compliance to take medication of hypertension patient of RSUD Penajam Paser Utar

BIMBINGAN TEKNIS PEMBUATAN HAND SANITIZER DARI BAHAN SINTETIS DAN ALAMI DI KELURAHAN TELUK LERONG ILIR DAN SIDODADI KECAMATAN SAMARINDA ULU

Melonjaknya penyebaran virus SARS-CoV-2 mengakibatkan harga hand sanitizer di Kalimantan Timur melonjak tinggi bahkan terjadi kekosongan stok hand sanitizer di pasaran baik pertokoan maupun fasilitas kesehatan. Tujuan Bimbingan Teknis (BimTek) pembuatan hand sanitizer dari bahan sintetis dan alami adalah peningkatan masyarakat mandiri yang inovatif dan kreatif di tengah masa pandemi Covid-19 hingga menuju kehidupan New Normal dalam menyikapi harga yang melonjak tinggi dan kekosongan stok hand sanitizer. Metode pelaksanaan bimbingan teknis ini dilakukan dengan penyampaian materi dan diskusi kepada peserta tentang definisi hand sanitizer, alat yang digunakan, komposisi bahan sintetis dan alami, cara pembuatan hand sanitizer berbahan sintetis dan alami, pengemasan dan  estimasi biaya yang dibutuhkan dalam pembuatannya. Hasil dari kegiatan BimTek ini menunjukkan bahwa peserta perwakilan dari Kelurahan Teluk Lerong dan Sidodadi Kecamatan Samarinda Ulu dalam Kegiatan BimTek dapat membuat hand sanitizer dari bahan sintetis dan alami dengan baik dan benar sesuai standar WHO dan penelitian sebelumnya yang sudah terpublikasi dalam jurnal ilmiah.

UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) PADA MENCIT PUTIH JANTAN (Mus musculus) DENGAN INDUKSI NYERI ASAM ASETAT

Kersen leaf (Muntingia calabura L.) contains tannins, flavonoids and polyphenol compounds allegedly have analgesic effect. The objective was to determine the analgesic effect of ethanol extract of kersen leaves and to determine the most effective analgesic dose. This study was an experimental research. Leaves were extracted with ethanol 70% and the analgesic effect test was divided into 5 groups: negative control treatment (distilled water), positive control (mefenamic acid 2.6mg/kg), kersen leaf ethanol extract first dose (100mg/kg), second dose (200mg/kg) and tthird dose (400mg/kg). Giving treatments by oral, after 30 minutes, the mices were given a pain inductor with 0.5% acetic acid by intra peritonial administration. Analgesic power was calculated by counting the number of writhing in mice for 1 hour. The results showed that the ethanol extract of cherry leaf has analgesic effect. From the calculation of the first dose analgesic power (42.9%), second dose (59.4%) and the third dose 69.9%. Statistical test results kruskal wallis value of p=0.011 (p<0.05) showed a significant difference between all analgesic treatment groups. The conclusion of this study is all of the ethanol extract had analgesic effects on male white mice, whereas a dose of 400mg/kg is the most effective analgesic dose

UJI AKTIVITAS EKSTRAK ETANOL DAUN ALPUKAT (Persea americana Mill.) TERHADAP PENYEMBUHAN LUKA BAKAR PADA PUNGGUNG MENCIT PUTIH JANTAN (Mus musculus)

Activity Assay of avocado leaf (Persea americana Mill.) ethanol extract on burn healing at back skin of white male mice (Mus musculus) aims to determine the best concentration of avocado leaf ethanol extract to healing burn on back skin of mice. Extract of avocado leaf obtained by maceration method using ethanol 70%. Burn made by soldering iron modified with square plate (size 1 x 1 cm2), during 2 seconds contact at the skin and make the skin formed blister. Observation of the burn area was doing daily for 14 days, and also the treatment with the 3 concentrations extract variation (20%,35%, and 50%), negative control and positive control. Result of research show that all avocado leaf ethanol extract in concentrations 20%, 35% and 50% have good activity in burn healing, from first day until 14th day they showed percentage of burn recovering successively equal to 86%, 88% and 90%. Negative control showed that process healing of burn was very tardy, only has percentage of burn recovering equal to 32,67% on 14th day, while positive control showed 100% burn healing on 11st day. The best concentration of  avocado leaf ethanol extract on healing burn is 50% with 90% burn healin

DEVELOPMENT OF PAPER-BASED COLOR TEST-STRIP FOR PARACETAMOL DETECTION IN JAMU

Chemical medicine which are synthetic chemicals or isolation products must not be added to herbal medicine, that was standared by Indonesian Head of the Drug and Food Control Agency in statement regarding Criteria and Procedure for Registration of Traditional Medicines, Standardized Herbal Medicines, and Phytofarmaka. This study aims to develope prototype of paper-based color test-strip to identify paracetamol trace in several types of Indonesian herbal medicine, better known as Jamu. Research conducted with experimental research wich make paper test-strip prototype by immobilizing reagents into Whatman’s filter paper, then it used to identify paracetamol in extracted Jamu samples. The paracetamol qualitative test uses 10 Jamu samples that distributed in Samarinda area. were also carried out on. Comparison methode with qualitative color reaction tests using specific reagents in the laboratory were also carried out beside the paper-based color test-strip methode. In addition with positive control tests using 5000 ppm paracetamol solution to make sure both methode used are valid. The results of this study showed that two prototype paper-based color test-strips containing 10% iron (III) chloride reagent and folin ciocalteu were able to detect the presence of paracetamol chemicals in Jamu samples with color reaction

UJI AKTIVITAS ANALGETIK EKSTRAK ETANOL DAUN SEREH WANGI (Cymbopogon nardus(L) Rendle) PADA MENCIT PUTIH (Mus musculus L) JANTAN DENGAN METODE INDUKSI NYERI CARA KIMIA

Pain is a sensory and emotional experience related to tissue damage. Fragrant lemongrass (Cymbopogon nardus) is widely used in Indonesia as a traditional medicine, especially used as a pain reliever. Therefor the use of these plants must be scientifically tested. The purpose of this test was to determine the ethanol extract of fragrant lemongrass’s leaves which had analgesic potential in male white mice (Mus musculus) and determine the optimal dose potentially provide analgetic activity. This test was an experimental study and conducted on 25 male white mice (Mus musculus) which were grouped randomly into 5 groups, including negative controls, positive control (Ibuprofen), ethanol extract of fragrant lemongrass’s leaves doses I (200 mg / kg), dose II (400 mg / kg), and dose III (800 mg / kg). Pain induction was performed chemically using perintoneal acetic acid after 30 minutes of treatment given. Analgesic power is calculated using the sum number of stretching mice for 1 hour. The results showed that ethanol extract of fragrant lemongrass’s leaves had analgesic activity at dose I (38.70%), dose II (51.84%) and dose III (59.51%). The optimal dose of ethanol extract of fragrant lemongrass’s leaves is at dose II (400 mg / kg) that had 51.84% analgesic effect. Keywords :    Pain, Cymbopogon nardus, analgesic activity test, male white mice, chemical way induction pai

Studi Perbandingan Hasil Sintesis Antara N-Amil Asetat dan Isoamil Asetat

Sintesis ester n-amil asetat dan isoamil asetat pada penelitian ini dilakukan melalui reaksi esterifikasi. n-Amil asetat disintesis dari n-amil alkohol dan asam asetat; isoamil asetat disintesis dari isoamil alkohol dan asam asetat. Keduanya menggunakan katalis asam sulfat pekat dan pemanasan selama 1,5 jam. Identifikasi hasil sintesis n-amil asetat dan isoamil asetat diperiksa dengan penetapan titik didih, penetapan indeks bias dan penetapan bobot jenis. Selanjutnya diperiksa dengan spektroskopi infra merah, spektroskopi resonansi magnetik inti proton dan spektroskopi resonansi magnetik inti karbon-13. Persentase hasil n-amil asetat yang diperoleh adalah 31,25 % dan persentase hasil isoamil asetat adalah 34,79 %

UJI AKTIVITAS EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) TERHADAP PENYEMBUHAN LUKA BAKAR PADA KULIT MENCIT PUTIH JANTAN (Mus musculus)

Kersen leaves (Muntingia calabura L.) is one kind of plants that have many utilities from bark, fruits to leaves. Kersen leaves contain flavonoids and tannins that have potential as a treatment for skin burn. Kersen leaves is used for a treatment such us for analgesic and anti-inflammatory. This study was an experimental research. The goal of this study was to know the potential of ethanol estracts of Kersen leaves against the healing of burn wounds on the back skin of white male mice. Twenty white male mice and were used in this experiment and were divided into 5 groups: control positive (ointment branded), control negative (vaseline flavum), ethanol extract of kersen leaves in vaseline flavum dose 2.6 mg , 5.2 mg and 10.4 mg . The backs of mice was induced using hot solder modified with stainless steel plate size 1 x 1 cm 2 for 2 seconds and the diameter of the healing skin burns was mensured. The results showed that the percentage of control positive was 100%, control negative (65%), ethanol extract of leaves of kersen dose 2.6 mg (84.3%), dose 5.2 mg (85.3%) and dose 10.4 mg (93.3%). The results of data were analyzed using statistical analysis. One - Sample Kolmogorov - Smirnov Test indicated that the data were normally distributed (0.768 > 0.05), the homogenity test (Test homogeneity of Variances) indicated that the data were not homogeneous (0.016 < 0.05). Kruskal Wallis test showed that five (5) treatment groups were significantly different and have the effect of healing of burn wound on the skin of male white mice (0.012 < 0.05), the Mann- Whitney test showed that the control positive and 10,4mg was significantly different (0.043 < 0.05)

UJI AKTIVITAS ANALGETIK EKSTRAK ETANOL HERBA SEMBUKAN (Paederia foetida L.) PADA MENCIT PUTIH JANTAN (Mus musculus) YANG DIINDUKSI DENGAN ASAM ASETAT

Tumbuhan sembukan (Paederia foetida L.) merupakan tumbuhan liar merambat yang biasanya digunakan oleh masyarakat berpotensi sebagai tumbuhan obat nyeri. Tujuan dari penelitian ini adalah mengetahui ekstrak etanol herba sembukan memiliki aktivitas analgetik pada mencit putih jantan (Mus musculus) dan mengetahui dosis optimal ekstrak etanol herba sembukan (Paederia foetida L.) yang berpotensi sebagai analgetik. Simplisia herba sembukan diekstraksi dengan pelarut etanol 70% dan dilakukan uji aktivitas analgetik yang dibagi dalam 5 kelompok perlakuan yaitu kelompok kontrol positif (kalium diklofenak), kontrol negatif, dosis I (80 mg/kg BB), dosis II (160 mg/kg BB) dan dosis III (320 mg/kg BB) dengan cara pemberian peroral. Tiga puluh menit setelah pemberian, mencit diberi indikator nyeri yaitu asam asetat 0,5%. Daya analgetik dihitung dengan menghitung jumlah geliat mencit selama 1jam. Dari hasil penelitian, ekstrak etanol herba sembukan memiliki aktivitas analgetik  pada  mencit putih jantan dengan persen daya analgetik dosis I 35,18%, dosis II 53,58% dan dosis III 68,98%. Dosis optimal yang berpotensi sebagai analgetik pada mencit putih jantan yaitu dosis III dengan persen daya analgetik sebesar 68,98%