Clustering of Bootstrap for Web Service Discovery

Web services are accessed using URLs in a distributed environment. WS WSDL document URLs are manually tabulated and clustered which increases the cost and timing for the developer. This paper introduces a new Clustering of URLs (CU) framework for clustering of bootstrap for web service discovery and clustering them in various domains using transfer, filter, spell check and domain set methods. These methods set them under the specific domain or general category. The CU framework is implemented with a sample URLs. The result shows the efficiency of the clustering of WSDL URLs

Methodology for Integrating Computational Tree Logic Model Checking in Unified Modelling Language Artefacts A Case Study of an Embedded Controller

A unified modelling language (UML) based formal verification methodology that can be easily integrated into an embedded system software development life cycle is suggested. The approach augments UML diagrams with formal models through an interfacing domain and adds semantics to these diagrams. The suggested methodology; commences from functional specification and use case modelling, selects the most critical behaviour where formal verification can add value to the development cycle, analyses the selected behaviour using UML state transition diagram, derives a state chart matrix from the same, and a high level language software translates the state chart matrix to a labelled transition system. Safety properties are derived from system specifications and are expressed as computation tree logic (CTL) formulae. CTL model-checking algorithm from the literature is used for model- checking. The applicability of the suggested approach is established using a safety critical embedded controller used for deployment and recovery of sensor structures from an airborne platform

Search Tracker: Human-derived object tracking in-the-wild through large-scale search and retrieval

Humans use context and scene knowledge to easily localize moving objects in conditions of complex illumination changes, scene clutter and occlusions. In this paper, we present a method to leverage human knowledge in the form of annotated video libraries in a novel search and retrieval based setting to track objects in unseen video sequences. For every video sequence, a document that represents motion information is generated. Documents of the unseen video are queried against the library at multiple scales to find videos with similar motion characteristics. This provides us with coarse localization of objects in the unseen video. We further adapt these retrieved object locations to the new video using an efficient warping scheme. The proposed method is validated on in-the-wild video surveillance datasets where we outperform state-of-the-art appearance-based trackers. We also introduce a new challenging dataset with complex object appearance changes.Comment: Under review with the IEEE Transactions on Circuits and Systems for Video Technolog

Anti-chaperone [eszett]A3/A1102-117 peptide interacting sites in human aB-crystallin

Purpose: Our previous work identified 23 low molecular weight (<3.5 kDa) crystallin peptides in the urea-soluble fractions of normal young, normal aged, and aged cataract human lenses. We found that one of these crystallin fragments, [beta]A3/ A1102-117 peptide (SDAYHIERLMSFRPIC), that are present in aged and cataract lens, increased the scattering of light by [beta]- and [gamma]-crystallins and alcohol dehydrogenase (ADH) and also reduced the chaperone-like activity of [alpha][beta]-crystallin. The present study was performed to identify the interacting sites of the [beta]A3/A1102-117 peptide in [alpha]B-crystallin. Methods: [beta]A3/A1102-117 peptide was first derivatized with sulfo-succinimidyl-2-[6-(biotinamido)-2-{pazidobenzamido}- hexanoamido] ethyl-1-3 dithio propionate (sulfo-SBED), a photoactivable, heterotrifunctional biotincontaining cross-linker. The biotin-derivatized peptide was then incubated with [alpha]B-crystallin at 37 [degrees]C for 2 h to allow complex formation followed by photolysis to facilitate the transfer of the biotin label from the peptide to [alpha]B-crystallin. Label transfer was confirmed by western blot, and the labeled [alpha]B-crystallin was digested with trypsin. Tryptic peptides from [alpha]B-crystallin carrying the biotin label were purified by avidin affinity chromatography, and [beta]A3/A1102-117 peptide interacting sites in [alpha]B-crystallin were identified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and nanospray quadrupole time-of-flight mass spectrometry (QqTOF MS/MS). Results: We found that the [beta]A3/A1102-117 peptide interacted with [alpha]B-crystallin regions 70LEKDR74, 83HFSPEELKVK92, 91VKVLGDVIEVHGK103, 93VLGDVIEVHGKHEER107, and 121KYR123, which are part of the [alpha]-crystallin domain, and were previously shown to be part of the functional chaperone site in [alpha]B-crystallin. The [beta]A3/A1102-117 peptide also interacted with regions at the COOH-terminal extension of [alpha]B-crystallin, 150KQVSGPER157, 164EEKPAVTAAPK174, and 164EEKPAVTAAPKK175. When two of the hydrophobic residues of [beta]A3/A1102-117 peptide were replaced with hydrophilic residues, the resulting substituted peptide, SDADHGERLMSFRPIC, did not show the anti-chaperone property. Conclusions: This study confirmed the interactions between a low molecular weight peptide derived from [beta]A3/A1- crystallin found in aged and cataract lenses and [alpha]B-crystallin. The binding of [beta]A3/A1102-117 peptide to the chaperone site and the COOH-terminal extension of [alpha]B-crystallin may explain its anti-chaperone property

Induction of apoptosis in human breast cancer cell line MCF-7 by phytochemicals from Gmelina asiatica

Currently, breast cancer is the leading cause of cancer-related death in women. Therefore, there is an urgent need to develop alternative therapeutic measures against this deadly disease. Many componentsfrom dietary or medicinal plants have been identified that possess substantial chemopreventive properties. India has unique plant varieties yet to be studied for anticancer components. Therefore, cytotoxicity activity and the mechanism of cell death exhibited by the extracts prepared from Gmelina asiatica, in human breast cancer MCF-7cells was investigated. The MCF-7 cells were seeded in 96-well culture plates in the presence and absence of different concentrations of extracts of G. asiatica to determinetheir anticancer effects using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Mechanism of cell death was evaluated by chromatin condensation using Hoest staining and morphological changes with the use of a contrast microscope. Plant extracts of G. asiatica were observed to induce apoptosis of MCF-7 cells as evidenced by MTT-cell proliferation assay, cell-morphological changes and chromatin condensation. The cytotoxicity of the chloroform extract was greater than other extracts of G. asiatica. The results of the present investigation is the first report on the potential anticancer activity of G. asiatica extracts and its possible mechanism of action on cancer cell proliferation in breast cancer MCF-7 cell lines

Synthesis of zinc oxide nanoparticles using plant leaf extract against urinary tract infection pathogen

In modern science, Nanotechnology is an ablaze field for the researchers. Zinc oxide nanoparticles (ZnO NPs) are known to be one of the most multifunctional inorganic nanoparticles with its application in treatment of urinary tract infection. Nanoparticles were synthesized using Passiflora caerulea fresh leaf extract and were characterized by UV-visible spectroscopy (UV-vis), X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), Energy dispersive analysis of x-ray (EDAX), Atomic force microscopy (AFM). Therefore, the study reveals an efficient, eco-friendly and simple method for the green synthesis of multifunctional ZnO NPs using P. caerulea. Urinary tract infection causing microbes were isolated from the disease affected patient urine sample. The synthesized nanoparticles have been tested against the pathogenic culture showed a very good zone of inhibition compared with plant extract. It indicates the biomedical capability of ZnO NPs

ACUTE TOXICITY STUDY AND THERAPEUTIC ACTIVITY OF MODIFIED ARJUNARISHTA ON ISOPROTERENOL-INDUCED MYOCARDIAL INFARCTION IN RATS

Objective: Ayurvedic formulation derived phytomedicine could bring a specific remedy against myocardial infarction (MI) without any side effects. Arjunarishta is a cardio tonic that nourishes and strengthens the myocardial muscle and promotes cardiac function. The preparation of Arjunarishta is modified and it does not involve fermentation. So it is alcohol-free and safe to all age groups. The study of acute toxicity and therapeutic activity of Modified Arjunarishta (MA) in isoproterenol (IPN) induced MI in rats was conducted to bring scientific evidence. Methods: Acute toxicity study: Mice are divided into three groups. Group I-control group; Group II and group III were test groups and they received an oral dose of 1000 mg/kg and 2000 mg/kg of MA, respectively. The experimental mice were observed for behaviour changes and clinical signs. Their body weight was also recorded. At the end of the experiment, blood sample was collected and glucose, liver function test (LFT), renal function test (RFT) and haematology parameters were analysed. Then they also subjected to gross pathological examination of all the major internal organs. Therapeutic study: Rats were divided into six groups. Group 1-normal control; Group 2 (induced)-IPN 85 mg/kg for the first two days; Group 3 (MA low dose)-received IPN as per group 2 followed by MA 200 mg/kg from the 3rd day to the end of the experiment; Group 4 (MA medium dose)-400 mg/kg; Group 5 (MA high dose)-600 mg/kg; Group 6 (Standard)-IPN as per group 2 followed by Arjunarishta 2 ml/kg body weight from the 3rd day to the end of the experiment. The collected serum sample was used for the estimation of myocardium-expressed proinflammatory cytokines. Heart tissue was homogenized for the estimation of calcium and lipid profile. Results: Acute toxicity: There were no signs of toxicity and no significant change in body weight. The value of glucose, RFT, LFT and haematological parameters are remained normal. Histopathological report showed normal architecture. Therapeutic activity: In the heart samples, significantly (p&lt;0.001) increased cholesterol, Triglyceride (TGL), Free Fatty acids (FFA) and calcium in IPN induced groups was noted. They are all significantly (p&lt;0.001) decreased in MA administrated groups of three different groups. In serum sample, a significantly (p&lt;0.001) increased cytokines of Tumor necrosis factor α (TNFα), Interlukins (IL-6, IL-1α and IL-1β) in IPN induced rats was recorded were as they get significantly (p&lt;0.001) decreased in MA administrated groups of three different doses. Conclusion: The results obtained from the acute toxicity experiment concluded that MA was found to be safe for oral administration. The therapeutic experiment results clearly emphasize the beneficial action of MA against IPN induced MI in rats