Parasexual analysis in Trichoderma reesei using protoplast fusion

Protoplast fusion techniques were used to induce parasexuality in Trichoderma reesei and the products of fusion and segregation analyzed in order to get genetic data regarding the three cellulase markers, exoglucanase (exo), endoglucanase (endo) and beta-glucosidase ( beta- glu) and their location with respect to other auxotrophic markers. Five mutants derived from T. reesei QM 9414, a hypercellulase producer, were used in this study

Common Representation of Information Flows for Dynamic Coalitions

We propose a formal foundation for reasoning about access control policies within a Dynamic Coalition, defining an abstraction over existing access control models and providing mechanisms for translation of those models into information-flow domain. The abstracted information-flow domain model, called a Common Representation, can then be used for defining a way to control the evolution of Dynamic Coalitions with respect to information flow

Energy Dependence of Parameters Characterizing Multiply Backscattering of Gamma Photons

The present studies aimed to investigate the effects of energy dependence of parameters characterizing multiply backscattering of gamma photons. The numbers of multiply backscattered events are found to be increasing with thickness of copper target, and saturate for a particular thickness known as saturation thickness. The saturation thickness is found to be decreasing with increase in incident gamma photon energy, and also is not altered by the variation in collimator opening. The number, energy and dose albedos, characterizing the reflection probability of a material, are also evaluated. For each of the incident gamma photon energy, the number and energy albedos show an increase with increasing target thickness, and finally saturate. Monte Carlo calculations support the results of present experimental work

Magnetically Targeted Endothelial Cell Localization in Stented Vessels

ObjectivesA novel method to magnetically localize endothelial cells at the site of a stented vessel wall was developed. The application of this strategy in a large animal model is described.BackgroundLocal delivery of blood-derived endothelial cells has been shown to facilitate vascular healing in animal models. Therapeutic utilization has been limited by an inability to retain cells in the presence of blood flow. We hypothesized that a magnetized stent would facilitate local retention of superparamagnetically labeled cells.MethodsCultured porcine endothelial cells were labeled with endocytosed superparamagnetic iron oxide microspheres. A 500:1 microsphere-to-cell ratio was selected for in vivo experiments based on bromo-deoxyuridine incorporation and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling assays. Stents were magnetized and implanted in porcine coronary and femoral arteries using standard interventional equipment. Labeled endothelial cells were delivered locally during transient occlusion of blood flow.ResultsThe delivered cells were found attached to the stent struts and were also distributed within the adjacent denuded vessel wall at 24 h.ConclusionsMagnetic forces can be used to rapidly place endothelial cells at the site of a magnetized intravascular stent. The delivered cells are retained in the presence of blood flow and also spread to the adjacent injured vessel wall. Potential applications include delivering a cell-based therapeutic effect to the local vessel wall as well as downstream tissue

Whitehead's varnish nasal pack

Whitehead's varnish is a little known but excellent nasal packing agent. We review available literature on the historical aspects and clinical use of Whitehead's varnish. Our personal experience with Whitehead's varnish is described, and we strongly recommend its use

Innovative Approaches To The Management of Acute Arterial Hypertension - Clevidipine Butyrate

Acute arterial hypertension is one of the major concerns in many clinical settings including but not limited to operating room, intensive care and emergency care units. Perioperative hypertension is one of the major reasons for cancellation of elective surgeries, and also increases the perioperative morbidity. We would like to discuss pathophysiology, evaluation of the patients with acute hypertension, management of these patients and future considerations of the current intravenous antihypertensive medications

Anomalous origin of the left coronary artery from the pulmonary artery: case report and review

Anomalous origin of the left coronary artery from the pulmonary artery is a rare congenital condition that proves to be fatal in most individuals during childhood due to significant left ventricular ischaemia. However, there are case reports of individuals surviving into adulthood that have varying presenting symptoms. We report a case of a young male, who presented to our cardiology clinic with typical ischaemic cardiac pain, with no established risk factors, and was found to have anomalous origin of the left coronary artery from the pulmonary artery that was subsequently surgically corrected

Parallax of PSR J1744-1134 and the Local Interstellar Medium

We present the annual trigonometric parallax of PSR J1744-1134 derived from an analysis of pulse times of arrival. The measured parallax, pi = 2.8+/-0.3 mas ranks among the most precisely determined distances to any pulsar. The parallax distance of 357+/-39 pc is over twice that derived from the dispersion measure using the Taylor & Cordes model for the Galactic electron distribution. The mean electron density in the path to the pulsar, n_e = (0.0088 +/- 0.0009) cm^{-3}, is the lowest for any disk pulsar. We have compared the n_e for PSR J1744-1134 with those for another 11 nearby pulsars with independent distance estimates. We conclude that there is a striking asymmetry in the distribution of electrons in the local interstellar medium. The electron column densities for pulsars in the third Galactic quadrant are found to be systematically higher than for those in the first. The former correlate with the position of the well known local HI cavity in quadrant three. The excess electrons within the cavity may be in the form of HII clouds marking a region of interaction between the local hot bubble and a nearby superbubble.Comment: revised version accepted for publication in ApJ Letters; reanalysis of uncertainty in parallax measure and changes to fig