3,826 research outputs found
Characterization of the casein gene complex in West Africa goats and description of a new αs1-casein polymorphism
The analysis of casein polymorphisms was carried
out in West Africa goat populations: Red Sokoto (n =
57), West African Dwarf Nigeria (n = 27), West African
Dwarf Cameroon (n = 39), and Borno (n = 37). The 4
casein genes alphas1 (CSN1S1), beta (CSN2), alphas2 (CSN1S2), and kappa (CSN3) were typed at the DNA level. No null alleles were found in any of the genes analyzed. A PCR single-strand conformation polymorphism method was
implemented for the identification of CSN1S1*F allele
simultaneously with A/01, B/E, N and the new allele.
The allele differed from CSN1S1*B by a synonymous
transversion TCG->TCT in the codon corresponding to
Ser66 of the mature protein. The new allele, named
CSN1S1*B', occurred at a high frequency in all the
populations, ranging from 0.295 (West African Dwarf
Cameroon) to 0.405 (Borno). A greater frequency was
found for alleles associated with high alphas1-casein quantity,
as has already been observed in the goat populations
from the Mediterranean area. The intermediate
E allele occurred only in the Red Sokoto and at a low
frequency. The faint F allele occurred in 3 populations
at frequencies lower than 0.03. Linkage disequilibrium
occurred in all the populations, with highly significant
differences in Borno, Red Sokoto, and West Africa
Dwarf Nigeria, and significant differences in West Africa
Dwarf Cameroon. Only 10 haplotypes showed frequencies
>= 0.05 in at least 1 of the 4 populations considered,
and the overall frequency was >0.1 only for 4
haplotypes: BAAB, B'ACA, ACAB, and BACA (in the
order CSN1S1-CSN2-CSN1S2-CSN3). Haplotype
BAAB, postulated as an ancestral haplotype in previous
studies, was the most common haplotype in all breeds
except Borno, where B'ACA was predominant. The results
obtained are of considerable significance given that very little information exists on the subject for African goats. The high frequency of strong alleles in the calcium-sensitive caseins as well as the high linkage disequilibrium found among the casein genes in the African breeds analyzed may suggest that specific casein haplotypes have already been selected due to their advantages for nutrition. Haplotypes providing greater
protein and casein content would increase the energy
content of milk, thus resulting in more favorable growth
and survival of young goats and humans consuming
the milk
Building a high-quality sense inventory for improved abbreviation disambiguation
Motivation: The ultimate goal of abbreviation management is to disambiguate every occurrence of an abbreviation into its expanded form (concept or sense). To collect expanded forms for abbreviations, previous studies have recognized abbreviations and their expanded forms in parenthetical expressions of bio-medical texts. However, expanded forms extracted by abbreviation recognition are mixtures of concepts/senses and their term variations. Consequently, a list of expanded forms should be structured into a sense inventory, which provides possible concepts or senses for abbreviation disambiguation
Elastic scattering of low energy pions by nuclei and the in-medium isovector pi N amplitude
Measurements of elastic scattering of 21.5 MeV pi+ and pi- by Si, Ca, Ni and
Zr were made using a single arm magnetic spectrometer. Absolute calibration was
made by parallel measurements of Coulomb scattering of muons. Parameters of a
pion-nucleus optical potential were obtained from fits to all eight angular
distributions put together. The `anomalous' s-wave repulsion known from pionic
atoms is clearly observed and could be removed by introducing a
chiral-motivated density dependence of the isovector scattering amplitude,
which also greatly improved the fits to the data. The empirical energy
dependence of the isoscalar amplitude also improves the fits to the data but,
contrary to what is found with pionic atoms, on its own is incapable of
removing the anomaly.Comment: 20 pages, 5 figures, 5 tables. V2 added details on
uncertainties,extended discussion. To appear in PR
Flexible Bioelectronic Devices Based on Micropatterned Monolithic Carbon Fiber Mats
Polymer-derived carbon can serve as an electrode material in multimodal neural stimulation, recording, and neurotransmitter sensing platforms. The primary challenge in its applicability in implantable, flexible neural devices is its characteristic mechanical hardness that renders it difficult to fabricate the entire device using only carbon. A microfabrication technique is introduced for patterning flexible, cloth-like, polymer-derived carbon fiber (CF) mats embedded in polyimide (PI), via selective reactive ion etching. This scalable, monolithic manufacturing method eliminates any joints or metal interconnects and creates electrocorticography electrode arrays based on a single CF mat. The batch-fabricated CF/PI composite structures, with critical dimension of 12.5 µm, are tested for their mechanical, electrical, and electrochemical stability, as well as to chemicals that mimic acute postsurgery inflammatory reactions. Their recording performance is validated in rat models. Reported CF patterning process can benefit various carbon microdevices that are expected to feature flexibility, material stability, and biocompatibility
Equatorial Pacific productivity changes near the Eocene-Oligocene boundary
There is general agreement that productivity in high latitudes increased in the late Eocene and remained high in the early Oligocene. Evidence for both increased and decreased productivity across the Eocene-Oligocene transition (EOT) in the tropics has been presented, usually based on only one paleoproductivity proxy and often in sites with incomplete recovery of the EOT itself. A complete record of the Eocene-Oligocene transition was obtained at three drill sites in the eastern equatorial Pacific Ocean (ODP Site 1218 and IODP Sites U1333 and U1334). Four paleoproductivity proxies that have been examined at these sites, together with carbon and oxygen isotope measurements on early Oligocene planktonic foraminifera, give evidence of ecologic and oceanographic change across this climatically important boundary. Export productivity dropped sharply in the basal Oligocene (~33.7�Ma) and only recovered several hundred thousand years later; however, overall paleoproductivity in the early Oligocene never reached the average levels found in the late Eocene and in more modern times. Changes in the isotopic gradients between deep- and shallow-living planktonic foraminifera suggest a gradual shoaling of the thermocline through the early Oligocene that, on average, affected accumulation rates of barite, benthic foraminifera, and opal, as well as diatom abundance near 33.5�Ma. An interval with abundant large diatoms beginning at 33.3�Ma suggests an intermediate thermocline depth, which was followed by further shoaling, a dominance of smaller diatoms, and an increase in average primary productivity as estimated from accumulation rates of benthic foraminifera
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