192 research outputs found
Laurent series expansion of a class of massive scalar one-loop integrals up to {\cal O}(\ep^2) in terms of multiple polylogarithms
In a recent paper we have presented results for a set of massive scalar
one-loop master integrals needed in the NNLO parton model description of the
hadroproduction of heavy flavors. The one--loop integrals were evaluated in
n=4-2\ep dimension and the results were presented in terms of a Laurent
series expansion up to {\cal O}(\ep^2). We found that some of the \ep^2
coefficients contain a new class of functions which we termed the
functions. The functions are defined in terms of one--dimensional integrals
involving products of logarithm and dilogarithm functions. In this paper we
derive a complete set of algebraic relations that allow one to convert the
functions of our previous approach to a sum of classical and multiple
polylogarithms. Using these results we are now able to present the \ep^2
coefficients of the one-loop master integrals in terms of classical and
multiple polylogarithms.Comment: 32 pages, Latex, references added, matches published versio
Density-functional theory investigation of oxygen adsorption at Pd(11N)(N=3,5,7) vicinal surfaces
We present a density-functional theory study addressing the on-surface
adsorption of oxygen at the Pd(11N) (N =3,5,7) vicinal surfaces, which exhibit
(111) steps and (100) terraces of increasing width. We find the binding to be
predominantly governed by the local coordination at the adsorption site. This
leads to very similar bonding properties at the threefold step sites of all
three vicinal surfaces, while the binding at the central fourfold hollow site
in the four atomic row terrace of Pd(117) is already very little disturbed by
the presence of the neighboring steps.Comment: 9 pages including 4 figures; related publications can be found at
http://www.fhi-berlin.mpg.de/th/th.htm
Short Term Performance of Bituminous Geomembranes with Respect to Temperature Variations
There is a significant lack of independentunbiased, peer-reviewed research on the performance of bituminous geomembranes in barrier system applications when compared to the standard barrier polymers used in industry today. The purpose of the research presented in this thesis is to help form a foundation of objective data on BGMbituminous geomembranes (BGMs)s performance in the field, as well as to compare it to polymer barriers like high density polyethylene (HDPE)HDPE geomembranes, LLDPElinear low density (LLDPE) geomembranes, and other well known material types. In addition, in Saskatchewan, the temperature can vary by more than 60Β°C so a variety of climate conditions need to be considered when installing geomembrane covers. Due to temperature variability, the influence on temperature on the performance of bituminous geomembranes is one of the main variables considered in this research.
A variety of different testing methods were evaluated during this research including multiple ASTM standards for geomembrane puncture and tearing, and short-term performance testing; all with variations in temperature included in the testing procedures. The ASTM style testing methods used the ASTM designated apparatus, while the short-term performance testing was performed using equipment designed and manufactured in the College of Engineering Shops.
When evaluating the puncture resistances of both BGMs and HDPE geomembranes, it was found that as temperatures increase, the displacement required to cause puncture also increasesthere was a positive correlation between the ambient temperature and the amount of displacement that occurs prior to puncture. In contrast to this, the puncture resistance of the samples themselves decrease as temperatures increase, while there was a negative correlation between the puncture resistance of the samples and the ambient temperature. During the sub-zero experiments, it was observed that the stiffness of the geomembranes increased. Additionally, while HDPE geomembranes showed higher overall loads at the time of puncture, the BGMs deformed in much more elastic manners allowing them to reform into their original shape due to the viscosityductility of the bitumen binding, which could be highly beneficial in application.
When looking at the short-term performance of BGMs, in applications with the presence of aggressive over liners with the potential to cause puncture, it was found that both the ES2 and ES4BGM samplesproducts were able to withstand applied stresses of up to 400 kPa without any holes being caused in the barrier. Additionally, at lower temperatures there was little to no surface deformation present on the samples, with very minimal damage from the aggressive over liner.
Based on the findings of this research, it was observed that HDPE retains a higher resistance to puncture than BGMs, however the highly viscous nature of BGMs shows that it could have many advantages in short-term performance depending on the conditions in which it is used
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Feasibility study for thixoforming nanostructured bainitic steels
Super Bainite Steel (0.74% C, 1.64% Si, 1.82% Mn, 1% Ni, 0.36% Mo, 0. 21% Cr, 0.047% Al bal. Fe) after cold plastic deformation was used as the starting material for the bainitic treatment preceded by controlled cooling from the thixoforming temperature range. The Differential Scanning Calorimetry was used to determine the amount of the liquid phase fraction vs temperature in the solidus-liquidus range. The steel was heated up to 1430 Β°C, which corresponded to about 30% of the liquid fraction. Then it was rapidly cooled in oil at three different temperatures: 240 Β°C, 270 Β°C and 300 Β°C. The samples were then held at those temperatures for 5 h. The microstructure of the samples after treatment at 240 Β°C showed globular grains (average size of 115 Β΅m), containing carbide-free bainite as a mixture of ferrite plates with average thickness of 63 nm, and retained austenite plates of thickness 40 nm. Plates of martensite and blocks of austenite were visible in some grain. X-ray studies confirmed the presence of 20.1 % of austenite and 79.9% of ferrite with martensite. The sample revealed the highest compression strength of 6651 MPa, at yield strength of 1780 MPa and compression strain Ξ΅=38%. With the increase of isothermal treatment temperature up to 270 ΛC and 300 ΛC, yield strength decreased to 1370 MPa, 1375 MPa and compression strength was 5243 MPa, 4138 MPa, respectively, while plastic strain reached 38.5% and 25 %, respectively. Higher temperature of bainitic treatment led to coarsening of super bainite plates and, in consequence to a decrease in mechanical properties. Initiation of crack propagation was observed at grains. They spread through the globules and eutectic, unlike in thixoformed tool steels, in which the crack path runs along the eutectic mixture between the globular grains. Preliminary studies of the corrosion resistance of Super Bainite steels containing carbide-free bainite structure were carried out. Results indicate slightly higher resistance in chlorides environment of the sample isothermally treated at 240 Β°C/5 h preceded by controlled cooling from thixoforming temperature range than the sample treated in the conventional way (1000 ΛC/15 min/240 ΛC/20 h).The research was supported by Polish science financial resources Applied Research Programme of the National Centre for Research and Development in Poland; βDeveloping a technology of producing complex elements by steel thixoformingβPBS1/B5/22/2013.This is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/j.msea.2015.10.08
Π ΠΠΠΠΠΠ§ΠΠ‘ΠΠΠ Π ΠΠΠΠ’Π ΠΠΠ Π‘Π ΠΠ. Π.Π. Π‘ΠΠΠΠ€ΠΠ‘ΠΠΠ‘ΠΠΠΠ
About clinical work in Sklifosovsky Research Institute for Emergency Medicine.ΠΡ ΠΏΠΎΠΈΠ½ΡΠ΅ΡΠ΅ΡΠΎΠ²Π°Π»ΠΈΡΡ ΠΌΠ½Π΅Π½ΠΈΠ΅ΠΌ Π·Π°ΠΌΠ΅ΡΡΠΈΡΠ΅Π»Ρ Π΄ΠΈΡΠ΅ΠΊΡΠΎΡΠ° ΠΠΠ Π‘Π ΠΈΠΌ. Π.Π. Π‘ΠΊΠ»ΠΈΡΠΎΡΠΎΠ²ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎ Π»Π΅ΡΠ΅Π±Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΠ΅ β Π³Π»Π°Π²Π½ΡΠΌ Π²ΡΠ°ΡΠΎΠΌ ΠΈΠ½ΡΡΠΈΡΡΡΠ° ΠΏΡΠΎΡΠ΅ΡΡΠΎΡΠΎΠΌ Π.Π. Π ΠΎΠ³Π°Π»Π΅ΠΌ ΠΎ Π½Π°ΡΡΠΎΡΡΠ΅ΠΌ ΡΠΎΡΡΠΎΡΠ½ΠΈΠΈ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΈΠ½ΡΡΠΈΡΡΡΠ°
Π ΠΠΠ§ΠΠΠΠΠ Π ΠΠΠΠ’Π ΠΠΠ Π‘ΠΠΠ ΠΠ ΠΠΠΠΠ©Π ΠΠ. Π.Π. Π‘ΠΠΠΠ€ΠΠ‘ΠΠΠ‘ΠΠΠΠ Π 2013 ΠΠΠΠ£
About clinical work in Sklifosovsky research Institute for Emergency Medicine in 2013.ΠΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅ΠΌ ΠΠ°ΡΠ΅ΠΌΡ Π²Π½ΠΈΠΌΠ°Π½ΠΈΡ ΠΌΠ½Π΅Π½ΠΈΠ΅ Π·Π°ΠΌΠ΅ΡΡΠΈΡΠ΅Π»Ρ Π΄ΠΈΡΠ΅ΠΊΡΠΎΡΠ° ΠΏΠΎ Π»Π΅ΡΠ΅Π±Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΠ΅ ΠΠΠ Π‘Π ΠΈΠΌ. Π.Π. Π‘ΠΊΠ»ΠΈΡΠΎΡΠΎΠ²ΡΠΊΠΎΠ³ΠΎ β Π³Π»Π°Π²Π½ΠΎΠ³ΠΎ Π²ΡΠ°ΡΠ° ΠΈΠ½ΡΡΠΈΡΡΡΠ° ΠΏΡΠΎΡΠ΅ΡΡΠΎΡΠ° ΠΠΈΡ
Π°ΠΈΠ»Π° ΠΠ΅ΠΎΠ½ΠΈΠ΄ΠΎΠ²ΠΈΡΠ° Π ΠΎΠ³Π°Π»Ρ ΠΎ Π»Π΅ΡΠ΅Π±Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΠ΅ ΠΠΠ ΡΠΊΠΎΡΠΎΠΉ ΠΏΠΎΠΌΠΎΡΠΈ ΠΈΠΌ. Π.Π. Π‘ΠΊΠ»ΠΈΡΠΎΡΠΎΠ²ΡΠΊΠΎΠ³ΠΎ Π² 2013 Π³ΠΎΠ΄Ρ
Laurent series expansion of a class of massive scalar one-loop integrals to ${\cal O}(\ep^2)
We use dimensional regularization to calculate the {\cal O}(\ep^2)
expansion of all scalar one-loop one-, two-, three- and four-point integrals
that are needed in the calculation of hadronic heavy quark production. The
Laurent series up to {\cal O}(\ep^2) is needed as input to that part of the
NNLO corrections to heavy flavor production at hadron colliders where the
one-loop integrals appear in the loop-by-loop contributions. The four-point
integrals are the most complicated. The {\cal O}(\ep^2) expansion of the
three- and four-point integrals contains in general polylogarithms up to and functions related to multiple polylogarithms of maximal weight and
depth four.Comment: 48 pages, 4 figures in the text, slight change in the title, one
reference added, matches published versio
ΠΠ»ΠΈΡΠ½ΠΈΠ΅ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠΎΠ² Π³Π΅Π½ΠΎΠ² CYP2D6 ΠΈ CYP2C9 Π½Π° ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΡΠ°ΠΌΠ°Π΄ΠΎΠ»Π° ΠΈ ΠΊΠ΅ΡΠΎΡΠΎΠ»Π°ΠΊΠ° ΠΏΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΡΠΎΡΠΎΠΊΠΎΠ»Π° ΡΡΠΊΠΎΡΠ΅Π½Π½ΠΎΠ³ΠΎ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π½Π΅ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½Π½ΡΠΌ ΠΎΡΡΡΡΠΌ ΠΊΠ°Π»ΡΠΊΡΠ»Π΅Π·Π½ΡΠΌ Ρ ΠΎΠ»Π΅ΡΠΈΡΡΠΈΡΠΎΠΌ, ΠΏΠ΅ΡΠ΅Π½Π΅ΡΡΠΈΡ Ρ ΠΎΠ»Π΅ΡΠΈΡΡΡΠΊΡΠΎΠΌΠΈΡ
Relevance. One of the key components of the accelerated recovery protocols (ARP), in addition to minimizing the surgical approach, is an adequate postoperative analgesia. Despite this, applied postoperative analgesia combinations are not devoid of drawbacks, such as lack of effective postoperative analgesia and the presence of side effect. The use of a pharmacogenetic approach to analgesic therapy for the purpose of its personalization may increase the effectiveness and safety of the use of analgesics. In particular, the presence of an inactive CYP2D6*4 allele , in which the conversion of tramadol to its active metabolite is reduced, contributes to the insufficient efficacy of the drug. As for non-steroidal anti-inflammatory drugs, the presence of CYP2C9*2/*3 polymorphisms leads to a decrease in drug metabolism and a longer half-life, resulting in the increase of the clinical effect and the risk of adverse reactions. Thus, genotyping of patients with the determination of the presence of specific genetic factors can rationalize the postoperative analgesia.Aim of study. Evaluation of the possible association of polymorphisms of the CYP2D6 and CYP2C9 genes with the clinical efficacy of tramadol and ketorolac in relation to postoperative pain.Material and methods. This observational clinical study involved 107 patients with uncomplicated acute calculous cholecystitis who underwent videolaparoscopic cholecystectomy and perioperative treatment according to ARP. All patients underwent whole blood sampling followed by real-time polymerase chain reaction genotyping. Analgesic efficacy was assessed using a visual analog scale (VAS) and McGill Pain Questionnaire.Results. In CYP2D64* carriers pain was higher than that of wild-type carriers, according to VAS and McGill Pain Questionnaire in all investigated periods. In carriers of CYP2C9*2, the pain syndrome was lower than in carriers of the wild type at all intervals studied. In carriers of CYP2C9*3 pain was lower only after 2 and 6 hours, also according to McGill Pain Questionnaire.Conclusion. 1. The presence of the polymorphic marker CYP2D6*4 may reduce the efficacy of postoperative tramadol analgesia compared with wild type. 2. The presence of the polymorphic marker CYP2C9*2 and CYP2C9*3 may increase the efficacy of ketorolac pain relief compared to wild type.ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ. ΠΠ΄Π½ΠΈΠΌ ΠΈΠ· ΠΊΠ»ΡΡΠ΅Π²ΡΡ
ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ² Π²ΡΠ΅Ρ
ΠΏΡΠΎΡΠΎΠΊΠΎΠ»ΠΎΠ² ΡΡΠΊΠΎΡΠ΅Π½Π½ΠΎΠ³ΠΎ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ (ΠΠ£Π), ΠΏΠΎΠΌΠΈΠΌΠΎ ΠΌΠΈΠ½ΠΈΠΌΠΈΠ·Π°ΡΠΈΠΈ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ Π΄ΠΎΡΡΡΠΏΠ°, ΡΠ²Π»ΡΠ΅ΡΡΡ Π°Π΄Π΅ΠΊΠ²Π°ΡΠ½Π°Ρ ΠΏΠΎΡΠ»Π΅ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½Π°Ρ Π°Π½Π°Π»Π³Π΅Π·ΠΈΡ. ΠΠ΅ΡΠΌΠΎΡΡΡ Π½Π° ΡΡΠΎ ΠΏΡΠΈΠΌΠ΅Π½ΡΠ΅ΠΌΡΠ΅ ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ ΠΏΠΎΡΠ»Π΅ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΎΠ±Π΅Π·Π±ΠΎΠ»ΠΈΠ²Π°Π½ΠΈΡ Π½Π΅ Π»ΠΈΡΠ΅Π½Ρ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΊΠΎΠ², ΡΠ°ΠΊΠΈΡ
ΠΊΠ°ΠΊ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½Π°Ρ ΠΏΠΎΡΠ»Π΅ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½Π°Ρ Π°Π½Π°Π»Π³Π΅Π·ΠΈΡ ΠΈ Π½Π°Π»ΠΈΡΠΈΠ΅ ΠΏΠΎΠ±ΠΎΡΠ½ΡΡ
ΡΡΡΠ΅ΠΊΡΠΎΠ² ΠΎΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΡΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ². ΠΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Π° ΠΊ ΠΎΠ±Π΅Π·Π±ΠΎΠ»ΠΈΠ²Π°ΡΡΠ΅ΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Ρ ΡΠ΅Π»ΡΡ Π΅Π΅ ΠΏΠ΅ΡΡΠΎΠ½Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΌΠΎΠΆΠ΅Ρ ΠΏΠΎΠ²ΡΡΠΈΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈ Π±Π΅Π·ΠΎΠΏΠ°ΡΠ½ΠΎΡΡΡ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ Π°Π½Π°Π»ΡΠ³Π΅ΡΠΈΠΊΠΎΠ². Π ΡΠ°ΡΡΠ½ΠΎΡΡΠΈ, Π½Π°Π»ΠΈΡΠΈΠ΅ Π½Π΅Π°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π°Π»Π»Π΅Π»Ρ CYP2D6*4, ΠΏΡΠΈ ΠΊΠΎΡΠΎΡΠΎΠΌ ΡΠ½ΠΈΠΆΠ΅Π½ΠΎ ΠΏΡΠ΅ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΡΠ°ΠΌΠ°Π΄ΠΎΠ»Π° Π² Π΅Π³ΠΎ Π°ΠΊΡΠΈΠ²Π½ΡΠΉ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡ, ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΠΉ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ°. Π§ΡΠΎ ΠΊΠ°ΡΠ°Π΅ΡΡΡ Π½Π΅ΡΡΠ΅ΡΠΎΠΈΠ΄Π½ΡΡ
ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ², Π½Π°Π»ΠΈΡΠΈΠ΅ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠΎΠ² CYP2C9*2/*3 ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° Π»Π΅ΠΊΠ°ΡΡΡΠ² ΠΈ Π±ΠΎΠ»Π΅Π΅ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌΡ ΠΏΠ΅ΡΠΈΠΎΠ΄Ρ ΠΏΠΎΠ»ΡΠ²ΡΠ²Π΅Π΄Π΅Π½ΠΈΡ, Π²ΡΠ»Π΅Π΄ΡΡΠ²ΠΈΠ΅ ΡΠ΅Π³ΠΎ ΠΌΠΎΠΆΠ΅Ρ ΡΡΠΈΠ»ΠΈΡΡΡΡ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΡΡΠ΅ΠΊΡ ΠΈ ΠΏΠΎΠ²ΡΡΠΈΡΡΡΡ ΡΠΈΡΠΊ Π½Π΅ΠΆΠ΅Π»Π°ΡΠ΅Π»ΡΠ½ΡΡ
ΡΠ΅Π°ΠΊΡΠΈΠΉ. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ Π³Π΅Π½ΠΎΡΠΈΠΏΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ΠΌ Π½Π°Π»ΠΈΡΠΈΡ ΠΊΠΎΠ½ΠΊΡΠ΅ΡΠ½ΡΡ
Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠΎΠ² ΠΌΠΎΠΆΠ΅Ρ ΡΠ°ΡΠΈΠΎΠ½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°ΡΡ ΠΏΠΎΡΠ»Π΅ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΡΡ Π°Π½Π°Π»Π³Π΅Π·ΠΈΡ.Π¦Π΅Π»Ρ. ΠΡΠ΅Π½ΠΊΠ° Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠΉ Π°ΡΡΠΎΡΠΈΠ°ΡΠΈΠΈ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠΎΠ² Π³Π΅Π½ΠΎΠ² CYP2D6 ΠΈ CYP2C9 Ρ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ ΡΡΠ°ΠΌΠ°Π΄ΠΎΠ»Π° ΠΈ ΠΊΠ΅ΡΠΎΡΠΎΠ»Π°ΠΊΠ° Π² ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ ΠΏΠΎΡΠ»Π΅ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π±ΠΎΠ»ΠΈ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π ΡΡΠΎΠΌ ΠΎΠ±ΡΠ΅ΡΠ²Π°ΡΠΈΠΎΠ½Π½ΠΎΠΌ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΡΠΈΠ½ΠΈΠΌΠ°Π»ΠΈ ΡΡΠ°ΡΡΠΈΠ΅ 107 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π½Π΅ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½Π½ΡΠΌ ΠΎΡΡΡΡΠΌ ΠΊΠ°Π»ΡΠΊΡΠ»Π΅Π·Π½ΡΠΌ Ρ
ΠΎΠ»Π΅ΡΠΈΡΡΠΈΡΠΎΠΌ, ΠΊΠΎΡΠΎΡΡΠΌ Π²ΡΠΏΠΎΠ»Π½ΡΠ»ΠΈ Π²ΠΈΠ΄Π΅ΠΎΠ»Π°ΠΏΠ°ΡΠΎΡΠΊΠΎΠΏΠΈΡΠ΅ΡΠΊΡΡ Ρ
ΠΎΠ»Π΅ΡΠΈΡΡΡΠΊΡΠΎΠΌΠΈΡ ΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΏΠ΅ΡΠΈΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ΅ Π»Π΅ΡΠ΅Π½ΠΈΠ΅ ΡΠΎΠ³Π»Π°ΡΠ½ΠΎ ΠΎΠΏΡΠΈΠΌΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΌΡ ΠΠ£Π. ΠΡΠ΅ΠΌ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ°ΠΌ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»ΡΡ Π·Π°Π±ΠΎΡ ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ Ρ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠΈΠΌ Π³Π΅Π½ΠΎΡΠΈΠΏΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½ΠΎΠΉ ΡΠ΅ΠΏΠ½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ Π² ΡΠ΅ΠΆΠΈΠΌΠ΅ ΡΠ΅Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ. ΠΠ±Π΅Π·Π±ΠΎΠ»ΠΈΠ²Π°ΡΡΠ°Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»Π°ΡΡ ΠΏΡΠΈ ΠΏΠΎΠΌΠΎΡΠΈ Π²ΠΈΠ·ΡΠ°Π»ΡΠ½ΠΎΠΉ Π°Π½Π°Π»ΠΎΠ³ΠΎΠ²ΠΎΠΉ ΡΠΊΠ°Π»Ρ (ΠΠΠ¨) ΠΈ ΠΎΠΏΡΠΎΡΠ½ΠΈΠΊΠ° Π±ΠΎΠ»ΠΈ ΠΠ°ΠΊ-ΠΠΈΠ»Π»Π°.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π£ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ CYP2D6*4 Π±ΠΎΠ»Π΅Π²ΠΎΠΉ ΡΠΈΠ½Π΄ΡΠΎΠΌ Π±ΡΠ» Π²ΡΡΠ΅, ΡΠ΅ΠΌ Ρ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ Π΄ΠΈΠΊΠΎΠ³ΠΎ ΡΠΈΠΏΠ°, ΠΏΠΎ Π΄Π°Π½Π½ΡΠΌ ΠΠΠ¨ ΠΈ Π±ΠΎΠ»Π΅Π²ΠΎΠ³ΠΎ ΠΎΠΏΡΠΎΡΠ½ΠΈΠΊΠ° ΠΠ°ΠΊ-ΠΠΈΠ»Π»Π° Π²ΠΎ Π²ΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΠ΅ ΠΏΡΠΎΠΌΠ΅ΠΆΡΡΠΊΠΈ. Π£ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ CYP2C9*2 Π±ΠΎΠ»Π΅Π²ΠΎΠΉ ΡΠΈΠ½Π΄ΡΠΎΠΌ Π±ΡΠ» Π½ΠΈΠΆΠ΅, ΡΠ΅ΠΌ Ρ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ Π΄ΠΈΠΊΠΎΠ³ΠΎ ΡΠΈΠΏΠ° Π²ΠΎ Π²ΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΠ΅ ΠΏΡΠΎΠΌΠ΅ΠΆΡΡΠΊΠΈ. Π£ Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ CYP2C9*3 Π±ΠΎΠ»Π΅Π²ΠΎΠΉ ΡΠΈΠ½Π΄ΡΠΎΠΌ Π±ΡΠ» Π½ΠΈΠΆΠ΅ ΡΠΎΠ»ΡΠΊΠΎ ΡΠ΅ΡΠ΅Π· 2 ΠΈ 6 ΡΠ°ΡΠΎΠ², Π° ΡΠ°ΠΊΠΆΠ΅ ΠΏΠΎ Π΄Π°Π½Π½ΡΠΌ ΠΎΡΠ΅Π½ΠΎΡΠ½ΠΎΠΉ ΡΠΊΠ°Π»Ρ Π±ΠΎΠ»ΠΈ ΠΠ°ΠΊ-ΠΠΈΠ»Π»Π°.ΠΡΠ²ΠΎΠ΄Ρ. 1. ΠΠ°Π»ΠΈΡΠΈΠ΅ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠΊΠ΅ΡΠ° CYP2D6*4 ΠΌΠΎΠΆΠ΅Ρ ΡΠ½ΠΈΠ·ΠΈΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΏΠΎΡΠ»Π΅ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΎΠ±Π΅Π·Π±ΠΎΠ»ΠΈΠ²Π°Π½ΠΈΡ ΡΡΠ°ΠΌΠ°Π΄ΠΎΠ»ΠΎΠΌ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π΄ΠΈΠΊΠΈΠΌ ΡΠΈΠΏΠΎΠΌ. 2. ΠΠ°Π»ΠΈΡΠΈΠ΅ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠΊΠ΅ΡΠ° CYP2C9*2 ΠΈ CYP2C9*3 ΠΌΠΎΠΆΠ΅Ρ ΠΏΠΎΠ²ΡΡΠΈΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΎΠ±Π΅Π·Π±ΠΎΠ»ΠΈΠ²Π°Π½ΠΈΡ ΠΊΠ΅ΡΠΎΡΠΎΠ»Π°ΠΊΠΎΠΌ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π΄ΠΈΠΊΠΈΠΌ ΡΠΈΠΏΠΎΠΌ
High resolution mapping and positional cloning of ENU-induced mutations in the Rw region of mouse chromosome 5
<p>Abstract</p> <p>Background</p> <p>Forward genetic screens in mice provide an unbiased means to identify genes and other functional genetic elements in the genome. Previously, a large scale ENU mutagenesis screen was conducted to query the functional content of a ~50 Mb region of the mouse genome on proximal Chr 5. The majority of phenotypic mutants recovered were embryonic lethals.</p> <p>Results</p> <p>We report the high resolution genetic mapping, complementation analyses, and positional cloning of mutations in the target region. The collection of identified alleles include several with known or presumed functions for which no mutant models have been reported (<it>Tbc1d14</it>, <it>Nol14</it>, <it>Tyms</it>, <it>Cad</it>, <it>Fbxl5</it>, <it>Haus3</it>), and mutations in genes we or others previously reported (<it>Tapt1</it>, <it>Rest</it>, <it>Ugdh</it>, <it>Paxip1</it>, <it>Hmx1, Otoe, Nsun7</it>). We also confirmed the causative nature of a homeotic mutation with a targeted allele, mapped a lethal mutation to a large gene desert, and localized a spermiogenesis mutation to a region in which no annotated genes have coding mutations. The mutation in <it>Tbc1d14 </it>provides the first implication of a critical developmental role for RAB-GAP-mediated protein transport in early embryogenesis.</p> <p>Conclusion</p> <p>This collection of alleles contributes to the goal of assigning biological functions to all known genes, as well as identifying novel functional elements that would be missed by reverse genetic approaches.</p
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