2 research outputs found
Propuesta de un sistema Haccp para el control de la calidad en el proceso de acondicionamiento de pota fresca en la empresa Jehov谩 mi buen pastor, 2019
El prop贸sito de la presente investigaci贸n es elaborar una Propuesta de sistema
HACCP para el control de calidad en el proceso de acondicionamiento de Pota
Fresca en la empresa Jehov谩 mi Buen Pastor. 脡sta investigaci贸n es descriptiva,
transversal de enfoque cualitativa, con dise帽o no experimental, la unidad de an谩lisis
fue el proceso de acondicionamiento de pota Fresca y como t茅cnica de recolecci贸n
de datos se us贸 la observaci贸n y la entrevista. En el desarrollo se sistematiz贸 y
orden贸 de acuerdo a lo normado por la Organizaci贸n de la Salud, siguiendo los
pasos del sistema HACCP. Se encontr贸 que se dan tres procesos principales, la
recepci贸n del producto, el proceso de eviscerado, limpieza-separaci贸n de cabezaaletas
y el despacho, describiendo y elaborando los diagramas de flujo de cada
proceso. Luego se determinaron los Puntos cr铆ticos de control y sus respectivos
l铆mites cr铆ticos para cada PCC, adem谩s los procedimientos para monitorearlos,
acciones correctivas a aplicar si es necesario, y el procedimiento de verificaci贸n
para asegurar la calidad del producto. Finalmente se propuso un sistema HACCP
conteniendo un manual del sistema HACCP y una Gu铆a de aplicaci贸n de buenas
pr谩cticas de manufactura (BPM
Direct association between pharyngeal viral secretion and host cytokine response in severe pandemic influenza
<p>Abstract</p> <p>Background</p> <p>Severe disease caused by 2009 pandemic influenza A/H1N1virus is characterized by the presence of hypercytokinemia. The origin of the exacerbated cytokine response is unclear. As observed previously, uncontrolled influenza virus replication could strongly influence cytokine production. The objective of the present study was to evaluate the relationship between host cytokine responses and viral levels in pandemic influenza critically ill patients.</p> <p>Methods</p> <p>Twenty three patients admitted to the ICU with primary viral pneumonia were included in this study. A quantitative PCR based method targeting the M1 influenza gene was developed to quantify pharyngeal viral load. In addition, by using a multiplex based assay, we systematically evaluated host cytokine responses to the viral infection at admission to the ICU. Correlation studies between cytokine levels and viral load were done by calculating the Spearman correlation coefficient.</p> <p>Results</p> <p>Fifteen patients needed of intubation and ventilation, while eight did not need of mechanical ventilation during ICU hospitalization. Viral load in pharyngeal swabs was 300 fold higher in the group of patients with the worst respiratory condition at admission to the ICU. Pharyngeal viral load directly correlated with plasma levels of the pro-inflammatory cytokines IL-6, IL-12p70, IFN-纬, the chemotactic factors MIP-1尾, GM-CSF, the angiogenic mediator VEGF and also of the immuno-modulatory cytokine IL-1ra (p < 0.05). Correlation studies demonstrated also the existence of a significant positive association between the levels of these mediators, evidencing that they are simultaneously regulated in response to the virus.</p> <p>Conclusions</p> <p>Severe respiratory disease caused by the 2009 pandemic influenza virus is characterized by the existence of a direct association between viral replication and host cytokine response, revealing a potential pathogenic link with the severe disease caused by other influenza subtypes such as H5N1.</p