Surface Electrostatics of Lipid Bilayers by EPR of a pH-Sensitive Spin-Labeled Lipid

AbstractMany biophysical processes such as insertion of proteins into membranes and membrane fusion are governed by bilayer electrostatic potential. At the time of this writing, the arsenal of biophysical methods for such measurements is limited to a few techniques. Here we describe a, to our knowledge, new spin-probe electron paramagnetic resonance (EPR) approach for assessing the electrostatic surface potential of lipid bilayers that is based on a recently synthesized EPR probe (IMTSL-PTE) containing a reversibly ionizable nitroxide tag attached to the lipids’ polar headgroup. EPR spectra of the probe directly report on its ionization state and, therefore, on electrostatic potential through changes in nitroxide magnetic parameters and the degree of rotational averaging. Further, the lipid nature of the probe provides its full integration into lipid bilayers. Tethering the nitroxide moiety directly to the lipid polar headgroup defines the location of the measured potential with respect to the lipid bilayer interface. Electrostatic surface potentials measured by EPR of IMTSL-PTE show a remarkable (within ±2%) agreement with the Gouy-Chapman theory for anionic DMPG bilayers in fluid (48°C) phase at low electrolyte concentration (50 mM) and in gel (17°C) phase at 150-mM electrolyte concentration. This agreement begins to diminish for DMPG vesicles in gel phase (17°C) upon varying electrolyte concentration and fluid phase bilayers formed from DMPG/DMPC and POPG/POPC mixtures. Possible reasons for such deviations, as well as the proper choice of an electrostatically neutral reference interface, have been discussed. Described EPR method is expected to be fully applicable to more-complex models of cellular membranes

Habitability: CAMELOT 4

During 1988 to 1989 the NASA/USRA Advanced Design Program sponsored research and design efforts aimed at developing habitability criteria and at defining a habitability concept as a useful tool in understanding and evaluating dwellings for prolonged stays in extraterrestrial space. The Circulating Auto sufficient Mars-Earth Luxurious Orbital Transport (CAMELOT) was studied as a case in which the students would try to enhance the quality of life of the inhabitants by applying architectural design methodology. The study proposed 14 habitability criteria considered necessary to fulfill the defined habitability concept, which is that state of equilibrium that results from the interaction between components of the Individual Architecture Mission Complex, which allows a person to sustain physiological homeostatis, adequate performance, and acceptable social relationships. Architecture, design development, refinements and revisions to improve the quality of life, new insights on artificial gravity, form and constitution problems, and the final design concept are covered

Toward a Meaning of Work

Contemporary observers of the occupation and social fabric contend that individuals as members of families need to assert their rights over their lives and their destinies. That the family and the workplace can be analyzed independently of each other\u27s existence or the dichotomy between the intrinsic value of work and the importance of non-work time for individuals and firms are both notions that are being considered contemporarily and complementary. This paper reviews the history and societal factors that affect the notion of-work and its utility as a focus for social policy students

Excitations in the quantum paramagnetic phase of the quasi-one-dimensional Ising magnet CoNb2_2O6_6 in a transverse field: Geometric frustration and quantum renormalization effects

The quasi-one-dimensional (1D) Ising ferromagnet CoNb$_2$O$_6$ has recently been driven via applied transverse magnetic fields through a continuous quantum phase transition from spontaneous magnetic order to a quantum paramagnet, and dramatic changes were observed in the spin dynamics, characteristic of weakly perturbed 1D Ising quantum criticality. We report here extensive single-crystal inelastic neutron scattering measurements of the magnetic excitations throughout the three-dimensional (3D) Brillouin zone in the quantum paramagnetic phase just above the critical field to characterize the effects of the finite interchain couplings. In this phase, we observe that excitations have a sharp, resolution-limited line shape at low energies and over most of the dispersion bandwidth, as expected for spin-flip quasiparticles. We map the full bandwidth along the strongly dispersive chain direction and resolve clear modulations of the dispersions in the plane normal to the chains, characteristic of frustrated interchain couplings in an antiferromagnetic isosceles triangular lattice. The dispersions can be well parametrized using a linear spin-wave model that includes interchain couplings and further neighbor exchanges. The observed dispersion bandwidth along the chain direction is smaller than that predicted by a linear spin-wave model using exchange values determined at zero field, and this effect is attributed to quantum renormalization of the dispersion beyond the spin-wave approximation in fields slightly above the critical field, where quantum fluctuations are still significant.Comment: 11 pages, 6 figures. Updated references. Minor changes to text and figure

Impact of noise on a dynamical system: prediction and uncertainties from a swarm-optimized neural network

In this study, an artificial neural network (ANN) based on particle swarm optimization (PSO) was developed for the time series prediction. The hybrid ANN+PSO algorithm was applied on Mackey--Glass chaotic time series in the short-term $x(t+6)$. The performance prediction was evaluated and compared with another studies available in the literature. Also, we presented properties of the dynamical system via the study of chaotic behaviour obtained from the predicted time series. Next, the hybrid ANN+PSO algorithm was complemented with a Gaussian stochastic procedure (called {\it stochastic} hybrid ANN+PSO) in order to obtain a new estimator of the predictions, which also allowed us to compute uncertainties of predictions for noisy Mackey--Glass chaotic time series. Thus, we studied the impact of noise for several cases with a white noise level ($\sigma_{N}$) from 0.01 to 0.1.Comment: 11 pages, 8 figure

Contextualizing the Relationship between Culture and Puerto Rican Health: Towards a Place-Based Framework of Minority Health Disparities

In both the culture of poverty literature and the acculturation literature, Puerto Ricans are portrayed in negative terms. The culture of poverty framework attributes Puerto Rican poverty to the mental, behavioral, and moral pathology of Puerto Rican individuals and to Puerto Rican culture. Similarly, outdated acculturation frameworks also trace the poor health of immigrants and racialized minorities, such as Puerto Ricans, to equivalent perceived deficiencies. In this paper, we argue that both the culture of poverty and acculturation frameworks are two pillars of the White Racial Frame (Feagin 2009) that sustains racial inequality in the United States. To build our case, we provide an overview of Puerto Rican physical health disparities and highlight key findings. Then, we analyze this literature using natural language processing (NLP) tools to examine the lexicon of words that scholars use to understand such disparities. Our literature review shows that Puerto Ricans are generally doing worse than other groups across a range of health indicators. Results from the NLP analyses reveal that the lexicon of the culture of poverty and outdated notions of acculturation are rhetorical tools that scholars still use to make sense of these conditions. We conclude by arguing that moving away from a White Racial Frame of Puerto Rican health requires a theoretical model that puts race, place, and culture within a multilevel framework that we call the Racialized Place Inequality Framework