Pre-implantation mouse embryos cultured In vitro under different oxygen concentrations show altered ultrastructures

Abstract Assisted Reproductive Technologies routinely utilize different culture media and oxygen (O2) concentrations to culture human embryos. Overall, embryos cultured under physiological O2 tension (5%) have improved development compared to embryos cultured under atmospheric O2 conditions (20%). The mechanisms responsible for this remain unclear. This study aimed to evaluate the effect of physiologic (5%) or atmospheric O2 (20%) tension on the microscopic ultrastructure of pre-implantation mouse embryos using Transmission Electron Microscopy (TEM). Embryos flushed out of the uterus after natural mating were used as the control. For use as the control, 2-cells, 4-cells, morulae, and blastocysts were flushed out of the uterus after natural fertilization. In vitro fertilization (IVF) was performed using potassium simplex optimized medium (KSOM) under different O2 tensions (5% and 20%) until the blastocyst stage. After collection, embryos were subjected to the standard preparative for light microscopy (LM) and TEM. We found that culture in vitro under 5% and 20% O2 results in an increase of vacuolated shaped mitochondria, cytoplasmic vacuolization and presence of multi-vesicular bodies at every embryonic stage. In addition, blastocysts generated by IVF under 5% and 20% O2 showed a lower content of heterochromatin, an interruption of the trophectodermal and inner cell mass cell membranes, an increased density of residual bodies, and high levels of glycogen granules in the cytoplasm. In conclusion, this study suggests that in vitro culture, particularly under atmospheric O2 tension, causes stage-specific changes in preimplantation embryo ultrastructure. In addition, atmospheric (20%) O2 is associated with increased alterations in embryonic ultrastructure; these changes may explain the reduced embryonic development of embryos cultured with 20% O2

STUDIO NUMERICO DELL'EMODINAMICA IN PAZIENTI CON DISSECAZIONE AORTICA DI TIPO B

The development of type B aortic dissection (AoD) is caused by altered haemodynamic forces exerted on false and true lumina of dissected aorta. This work aims to study haemodynamic and morphological proprieties, which influence the progression or stability of type B AoD. Computational fluid-dynamic analyses were performed on three patients with type B AoD, whose two presented an aneurysm evolution and one presented no further complication connected with dissection. Blood flow features showed that the true lumen flow was laminar and uniform while false lumen flow lost the laminar characteristic with consequent vorticity formation. The entry point zone proximal to the aortic arch showed both high blood velocity and pressure connected with peaks of maximum WSS value. A negative pressure gradient between the false lumen entry and re-entry points was observed. This promotes the blood to flow towards the abdominal aorta reducing the risk of retrograde flow during systole. We concluded that fluid dynamics study of patients with type B AoD is a valid tool to identify the risk of aortic dissection progression as well as the formation of aortic dissection connected pathology

A NEW HANDHELD SCANNER FOR 3D SURVEY OF SMALL ARTIFACTS: THE STONEX F6

Movable heritage preserved in our museums are an invaluable evidence of our past. In order to properly respond to the need of 3D documentation of these significant assets, in the last few years both range-based and image-based solutions have been developed by researchers operating in the framework of Geomatics with a special focus on reaching a high level of detail and on texture radiometric quality, taking into consideration the intrinsic fragility of these kinds of objects which during the survey require a contactless approach. During the presented research a collection of architectural models representing ancient Nubian temples from “Museo Egizio di Torino” had been digitalized using different techniques; in particular, the wooden maquette of the temple of El-Hilla has been acquired using a new structured light handheld laser scanner, the Stonex F6 SR, and applying a close-range photogrammetric approach. In this paper a comparison between the two approaches is proposed as regards acquisition workflow, final results and suitability as regards digitisation of objects belonging to movable heritage and museum collections

Metric documentation of cultural heritage: Research directions from the Italian gamher project

GAMHer is a collaborative project that aims at exploiting and validating Geomatics algorithms, methodologies and procedures in the framework of new European regulations, which require a more extensive and productive use of digital information, as requested by the Digital Agenda for Europe as one of the seven pillars of the Europe 2020 Strategy. To this aim, GAMHer focuses on the need of a certified accuracy for surveying and monitoring projects with photogrammetry and laser scanning technologies, especially when used in a multiscale approach for landscape and built heritage documentation, conservation, and management. The approach used follows a multi-LoD (level of detail) transition that exploits GIS systems at the landscape scale, BIM technology and "point cloud based" 3d modelling for the scale of the building, and an innovative BIM/GIS integrated approach to foster innovation, promote users' collaboration and encourage communication between users. The outcomes of GAMHer are not intended to be used only by a community of Geomatics specialists, but also by a heterogeneous user community that exploit images and laser scans in their professional activities

METRIC DOCUMENTATION OF CULTURAL HERITAGE: RESEARCH DIRECTIONS FROM THE ITALIAN GAMHER PROJECT

GAMHer is a collaborative project that aims at exploiting and validating Geomatics algorithms, methodologies and procedures in the framework of new European regulations, which require a more extensive and productive use of digital information, as requested by the Digital Agenda for Europe as one of the seven pillars of the Europe 2020 Strategy. To this aim, GAMHer focuses on the need of a certified accuracy for surveying and monitoring projects with photogrammetry and laser scanning technologies, especially when used in a multiscale approach for landscape and built heritage documentation, conservation, and management. The approach used follows a multi-LoD (level of detail) transition that exploits GIS systems at the landscape scale, BIM technology and "point cloud based" 3d modelling for the scale of the building, and an innovative BIM/GIS integrated approach to foster innovation, promote users' collaboration and encourage communication between users. The outcomes of GAMHer are not intended to be used only by a community of Geomatics specialists, but also by a heterogeneous user community that exploit images and laser scans in their professional activities

MULTI-SOURCE APPROACHES FOR COMPLEX ARCHITECTURE DOCUMENTATION: THE “PALAZZO DUCALE” IN GUBBIO (PERUGIA, ITALY)

Nowadays, the employment of rapid mapping solutions for architectural survey is more and more considered, not only for the strong reduction of the primary data acquisition times, but also thanks to their adaptability to various contexts, especially in the framework of Cultural Heritage documentation where tailored solutions are required. The combined use of Mobile Mapping Systems (MMS), Unmanned Aerial Vehicles (UAV) for close range aerial image acquisition, and Terrestrial Laser Scanning (TLS) seems to be an effective solution for the architectonic scale compliant to the level of detail and accuracy of 1 : 200, and 1 : 100 scales. The present research tries to evaluate the use of a handheld MMS, the ZEB-Revo RT by Geoslam, an UAV, the DJI Mavic Pro, and a LiDAR system, the Faro Focus3D S 120 by CAM2. The complex case of the documentation metric survey of Palazzo Ducale in Gubbio, the Montefeltro’s Palace, now hosting the museum of itself, allows the comparison of the effectiveness of the used technologies

Addressing the Smart Systems Design Challenge: The SMAC Platform

This article presents the concepts, the organization, and the preliminary application results of SMAC, a smart systems co-design platform. The SMAC platform, which has been developed as Integrated Project (IP) of the 7th ICT Call under the Objective 3.2 \u201cSmart components and Smart Systems integration\u201d addresses the challenges of the integration of heterogeneous and conflicting domains that emerge in the design of smart systems. SMAC includes methodologies and EDA tools enabling multi-disciplinary and multi-scale modelling and design, simulation of multidomain systems, subsystems and components at different levels of abstraction, system integration and exploration for optimization of functional and non-functional metrics. The article presents the preliminary results obtained by adopting the SMAC platform for the design of a limb tracking smart system

An Experimental Evaluation of Resistive Defects and Different Testing Solutions in Low-Power Back-Biased SRAM Cells

This paper compares different types of resistive defects that may occur inside low-power SRAM cells, focusing on their impact on device operation. Notwithstanding the continuous evolution of SRAM device integration, manufacturing processes continue to be very sensitive to production faults, giving rise to defects that can be modeled as resistances, especially for devices designed to work in low-power modes. This work analyzes this type of resistive defect that may impair the device functionalities in subtle ways, depending on the defect characteristics and values that may not be directly or easily detectable by traditional test methods. We analyze each defect in terms of the possible effects inside the SRAM cell, its impact on power consumption, and provide guidelines for selecting the best test methods