AN ANALYSIS OF IODINE DEFICIENCY DISORDER AND ERADICATION STRATEGIES IN THE HIGH ATLAS MOUNTAINS OF MOROCCO

Food Consumption/Nutrition/Food Safety, Health Economics and Policy,

Biochemical Variation Among Normal Equine Carpal and Tarsocrural Joint Fluids are Detected by Infrared Spectral Characteristics and A Modified Approach to Linear Discriminant Analysis

Research into osteoarthritis diagnostics has evolved from traditional methods that are only useful in more advanced clinical disease, towards the discovery of biomarkers that are predictive or reflective of preclinical joint disease. The potential of Fourier transform infrared spectroscopy (FTIR) coupled with chemometrics has been demonstrated as useful for the assessment of biomolecular responses to disease. Joint fluid was collected from 105 clinically normal antebrachiocarpal (AC), midcarpal (MC) and tarsocrural (TCRL) joints. Thin films were prepared and FTIR absorbance spectra in the mid-infrared region recorded. Interferograms were signal averaged and Fourier transformed to generate spectra with a nominal resolution of 4 cm-1. Comparisons among joints were made using a novel modified method similar to linear discriminant analysis, which maximized the difference of between-group variance minus within-group variance, followed by permutation testing. Differences within animal between contralateral pairs of joints were minimal. Significant differences among AC, MC and TCRL joint fluid spectra were found. The range of biomolecular differences among these normal joints as characterized by FTIR indicates that interarticular variation within the horse needs to be considered for ongoing research, especially when utilizing within-horse joints as controls

Infrared Thermography for the Ante Mortem Detection of Bruising in Horses Following Transport to a Slaughter Plant

Undetected injury of horses sustained during road transport to slaughter is a welfare concern. This study evaluated digital infrared thermography (DT) for the detection of ante-mortem bruising in horses following transport to a slaughter plant. The sensitivity and specificity of DT for the detection of bruises following transport was evaluated. DT images were obtained from 93 horses (2–3 horses per load; 40 loads) at a Canadian federally approved slaughter plant. From an elevated platform 5 m from the horses, left and right lateral DT images, and one caudal pelvic area image were obtained from each horse. After slaughter the carcasses were examined for bruising (a visually discolored area on the carcass caused by damage to the blood vessels) and findings documented. Sensitivity, specificity, and predictive values were calculated for DT assessment of bruising. The prevalence of bruising on post mortem inspection was 54%. The DT approach to bruise detection at the region of interest level of 93 horses (n = 186 sides) resulted in a sensitivity of 42% and specificity of 79%. As the sensitivity was low, a more sensitive DT camera and allowing for a longer equilibration time for horses after transport may improve this approach to post transport assessment of subclinical injury

Equine Transport-Related Problem Behaviors and Injuries: A Survey of Italian Horse Industry Members

An online survey was conducted to determine associations between equine transport management and transport-related injuries and problem behaviors in Italy. The survey was composed of four sections: respondents\u2019 demographic information and background, transport management practices, journey details and vehicle design, and transport injuries experienced by the horse in the previous two-year period. Univariable and multivariable logistic regression with a binary outcome variable was performed to explore associations between variables (respondents\u2019 and journeys\u2019 details and transport practices) and equine transport-related problem behaviors (TRPBs) and injuries. TRPBs were also considered an explanatory variable for injuries. The survey generated 201 responses; only 148 were complete and analyzed. TRPBs were reported by 14.45% of the respondents and the odds of TRPBs was linked to the respondent gender (p = 0.034), the use of tranquilizers prior to transport (p = 0.002), the use of a whip for loading (p = 0.049), the lack of protection equipment (p = 0.050), and shavings (p = 0.025) on the vehicle floor. Horse injuries (11.49%) were reported by more respondents who did not check the brakes of their transport vehicle before traveling (p = 0.043), had vehicles with padding on the chest bar (p = 0.038), and for horses reported to display TRPBs (p = 0.001). Finally, 10 respondents reported they were injured during horse transport (10/140; 7.14%), 50% simultaneously with their horses. The study findings should be interpreted with caution due to small sample size bias and participants\u2019 recall bias. Nevertheless, the results are in concordance with the literature, confirming that horse transport is a risk for the horse\u2019s and handler\u2019s health and well-being. Further studies are needed to identify best management practices to educate equine industry members on how to minimize transport-related problems

Reliability and validity of the Spanish version of the Child Health and Illness Profile (CHIP) Child-Edition, Parent Report Form (CHIP-CE/PRF)

<p>Abstract</p> <p>Background</p> <p>The objectives of the study were to assess the reliability, and the content, construct, and convergent validity of the Spanish version of the CHIP-CE/PRF, to analyze parent-child agreement, and compare the results with those of the original U.S. version.</p> <p>Methods</p> <p>Parents from a representative sample of children aged 6-12 years were selected from 9 primary schools in Barcelona. Test-retest reliability was assessed in a convenience subsample of parents from 2 schools. Parents completed the Spanish version of the CHIP-CE/PRF. The Achenbach Child Behavioural Checklist (CBCL) was administered to a convenience subsample.</p> <p>Results</p> <p>The overall response rate was 67% (n = 871). There was no floor effect. A ceiling effect was found in 4 subdomains. Reliability was acceptable at the domain level (internal consistency = 0.68-0.86; test-retest intraclass correlation coefficients = 0.69-0.85). Younger girls had better scores on Satisfaction and Achievement than older girls. Comfort domain score was lower (worse) in children with a probable mental health problem, with high effect size (ES = 1.45). The level of parent-child agreement was low (0.22-0.37).</p> <p>Conclusions</p> <p>The results of this study suggest that the parent version of the Spanish CHIP-CE has acceptable psychometric properties although further research is needed to check reliability at sub-domain level. The CHIP-CE parent report form provides a comprehensive, psychometrically sound measure of health for Spanish children 6 to 12 years old. It can be a complementary perspective to the self-reported measure or an alternative when the child is unable to complete the questionnaire. In general, the results are similar to the original U.S. version.</p

Large and seasonally varying biospheric CO₂ fluxes in the Los Angeles megacity revealed by atmospheric radiocarbon

Measurements of Δ¹⁴C and CO₂ can cleanly separate biogenic and fossil contributions to CO₂ enhancements above background. Our measurements of these tracers in air around Los Angeles in 2015 reveal high values of fossil CO₂ and a significant and seasonally varying contribution of CO₂ from the urban biosphere. The biogenic CO₂ is composed of sources such as biofuel combustion and human metabolism and an urban biospheric component likely originating from urban vegetation, including turf and trees. The urban biospheric component is a source in winter and a sink in summer, with an estimated amplitude of 4.3 parts per million (ppm), equivalent to 33% of the observed annual mean fossil fuel contribution of 13 ppm. While the timing of the net carbon sink is out of phase with wintertime rainfall and the sink seasonality of Southern California Mediterranean ecosystems (which show maximum uptake in spring), it is in phase with the seasonal cycle of urban water usage, suggesting that irrigated urban vegetation drives the biospheric signal we observe. Although 2015 was very dry, the biospheric seasonality we observe is similar to the 2006–2015 mean derived from an independent Δ¹⁴C record in the Los Angeles area, indicating that 2015 biospheric exchange was not highly anomalous. The presence of a large and seasonally varying biospheric signal even in the relatively dry climate of Los Angeles implies that atmospheric estimates of fossil fuel–CO₂ emissions in other, potentially wetter, urban areas will be biased in the absence of reliable methods to separate fossil and biogenic CO₂

Large and seasonally varying biospheric CO₂ fluxes in the Los Angeles megacity revealed by atmospheric radiocarbon

Measurements of Δ¹⁴C and CO₂ can cleanly separate biogenic and fossil contributions to CO₂ enhancements above background. Our measurements of these tracers in air around Los Angeles in 2015 reveal high values of fossil CO₂ and a significant and seasonally varying contribution of CO₂ from the urban biosphere. The biogenic CO₂ is composed of sources such as biofuel combustion and human metabolism and an urban biospheric component likely originating from urban vegetation, including turf and trees. The urban biospheric component is a source in winter and a sink in summer, with an estimated amplitude of 4.3 parts per million (ppm), equivalent to 33% of the observed annual mean fossil fuel contribution of 13 ppm. While the timing of the net carbon sink is out of phase with wintertime rainfall and the sink seasonality of Southern California Mediterranean ecosystems (which show maximum uptake in spring), it is in phase with the seasonal cycle of urban water usage, suggesting that irrigated urban vegetation drives the biospheric signal we observe. Although 2015 was very dry, the biospheric seasonality we observe is similar to the 2006–2015 mean derived from an independent Δ¹⁴C record in the Los Angeles area, indicating that 2015 biospheric exchange was not highly anomalous. The presence of a large and seasonally varying biospheric signal even in the relatively dry climate of Los Angeles implies that atmospheric estimates of fossil fuel–CO₂ emissions in other, potentially wetter, urban areas will be biased in the absence of reliable methods to separate fossil and biogenic CO₂