THE PROMISED CONSTITUTION OF THE PROMISED LAND:THE ISRAELI CONSTITUTIONAL EXPERIENCE

THE PROMISED CONSTITUTION OF THE PROMISED LAND:THE ISRAELI CONSTITUTIONAL EXPERIENC

Hyperglycemia-induced abnormalities in rat and human corneas: the potential of second harmonic generation microscopy.

BACKGROUND: Second Harmonic Generation (SHG) microscopy recently appeared as an efficient optical imaging technique to probe unstained collagen-rich tissues like cornea. Moreover, corneal remodeling occurs in many diseases and precise characterization requires overcoming the limitations of conventional techniques. In this work, we focus on diabetes, which affects hundreds of million people worldwide and most often leads to diabetic retinopathy, with no early diagnostic tool. This study then aims to establish the potential of SHG microscopy for in situ detection and characterization of hyperglycemia-induced abnormalities in the Descemet's membrane, in the posterior cornea. METHODOLOGY/PRINCIPAL FINDINGS: We studied corneas from age-matched control and Goto-Kakizaki rats, a spontaneous model of type 2 diabetes, and corneas from human donors with type 2 diabetes and without any diabetes. SHG imaging was compared to confocal microscopy, to histology characterization using conventional staining and transmitted light microscopy and to transmission electron microscopy. SHG imaging revealed collagen deposits in the Descemet's membrane of unstained corneas in a unique way compared to these gold standard techniques in ophthalmology. It provided background-free images of the three-dimensional interwoven distribution of the collagen deposits, with improved contrast compared to confocal microscopy. It also provided structural capability in intact corneas because of its high specificity to fibrillar collagen, with substantially larger field of view than transmission electron microscopy. Moreover, in vivo SHG imaging was demonstrated in Goto-Kakizaki rats. CONCLUSIONS/SIGNIFICANCE: Our study shows unambiguously the high potential of SHG microscopy for three-dimensional characterization of structural abnormalities in unstained corneas. Furthermore, our demonstration of in vivo SHG imaging opens the way to long-term dynamical studies. This method should be easily generalized to other structural remodeling of the cornea and SHG microscopy should prove to be invaluable for in vivo corneal pathological studies

Socialization of inpatriate managers to the headquarters of global organizations: An application of reference point theory

Understanding the intricacies of managing the life cycle of international personnel is a conundrum that continues to perplex HR managers in the global business arena. Although the notion of adjusting to a socioeconomically and culturally distant environment has been explored extensively from the expatriate perspective, the critical issue to discern is the attention inpatriates need to facilitate successful, long-term integration into a novel setting. With regard to the relatively new staffing option of "inpatriation," the current literature seeks to acquire an understanding of the contextual implications vital for an adjustment process that allows for the successful and lasting incorporation of such individuals in the headquarters of global organizations. As a result of this gap, this article examines the relationship between institutional and individualized socialization tactics and sociocultural and psychological adjustments in conjunction with a proposed moderator effect of cultural distance that inpatriates may undergo. Overall, the article argues that the successful socializing of such individuals will in part depend on their set of psychological reference points during the acculturation stages. © 2010 SAGE Publications