Using CFD to improve the design of a circulating water channel

Computational Fluid Dynamics (CFD) has been used as a design tool to investigate means of improving flow uniformity in the working section of a circulating water channel. The CFD model was based on a 1/10th scale wind-tunnel model of the circulating water channel at the Australian Maritime Hydrodynamics Research Centre (AMHRC). The CFD analysis was compared with experimental results obtained from the wind-tunnel model to validate the use of the CFD model. Three changes to the design were investigated; alteration of turning vane angle, increased resistance coefficient of a honeycomb screen and addition of trailing edge extensions to the turning vanes. The turning vane angle changes resulted in little improvement in flow uniformity. Increasing the resistance coefficient of the honeycomb screen resulted in improved uniformity, but at the expense of increased pressure loss. The addition of trailing edge extensions to the turning vanes resulted in the most significant improvements in flow uniformity. These results will be useful in selecting improvements to the circulating water channel

Natural and Induced Mitochondrial Phosphate Carrier Loss: DIFFERENTIAL DEPENDENCE OF MITOCHONDRIAL METABOLISM AND DYNAMICS AND CELL SURVIVAL ON THE EXTENT OF DEPLETION.

The relevance of mitochondrial phosphate carrier (PiC), encoded by SLC25A3, in bioenergetics is well accepted. However, little is known about the mechanisms mediating the cellular impairments induced by pathological SLC25A3 variants. To this end, we investigated the pathogenicity of a novel compound heterozygous mutation in SLC25A3 First, each variant was modeled in yeast, revealing that substituting GSSAS for QIP within the fifth matrix loop is incompatible with survival on non-fermentable substrate, whereas the L200W variant is functionally neutral. Next, using skin fibroblasts from an individual expressing these variants and HeLa cells with varying degrees of PiC depletion, PiC loss of ∼60% was still compatible with uncompromised maximal oxidative phosphorylation (oxphos), whereas lower maximal oxphos was evident at ∼85% PiC depletion. Furthermore, intact mutant fibroblasts displayed suppressed mitochondrial bioenergetics consistent with a lower substrate availability rather than phosphate limitation. This was accompanied by slowed proliferation in glucose-replete medium; however, proliferation ceased when only mitochondrial substrate was provided. Both mutant fibroblasts and HeLa cells with 60% PiC loss showed a less interconnected mitochondrial network and a mitochondrial fusion defect that is not explained by altered abundance of OPA1 or MFN1/2 or relative amount of different OPA1 forms. Altogether these results indicate that PiC depletion may need to be profound (\u3e85%) to substantially affect maximal oxphos and that pathogenesis associated with PiC depletion or loss of function may be independent of phosphate limitation when ATP requirements are not high

New Young Star Candidates in BRC 27 and BRC 34

We used archival Spitzer Space Telescope mid-infrared data to search for young stellar objects (YSOs) in the immediate vicinity of two bright-rimmed clouds, BRC 27 (part of CMa R1) and BRC 34 (part of the IC 1396 complex). These regions both appear to be actively forming young stars, perhaps triggered by the proximate OB stars. In BRC 27, we find clear infrared excesses around 22 of the 26 YSOs or YSO candidates identified in the literature, and identify 16 new YSO candidates that appear to have IR excesses. In BRC 34, the one literature-identified YSO has an IR excess, and we suggest 13 new YSO candidates in this region, including a new Class I object. Considering the entire ensemble, both BRCs are likely of comparable ages, within the uncertainties of small number statistics and without spectroscopy to confirm or refute the YSO candidates. Similarly, no clear conclusions can yet be drawn about any possible age gradients that may be present across the BRCs.Comment: 54 pages, 19 figures, accepted by A

Hepatoma in Intact C3Hf Male and Virgin Female Mice and after Gonadectomy alone or subsequent Treatment with Oestrogen

AN excess of spontaneous hepatoma in male over breeding CBA female mice

Patterns of Water: Resource Pack

This is a resource pack that we have put together from the cluster analysis results of a survey, and qualitative interviews, conducted across the south and south east of England exploring practices using water in the home and garden. We are happy for you to use this resource pack in policy, business, and teaching however please reference as: Browne, A.L., Pullinger, M., Medd, W., & Ander-son, B. (2013). Patterns of Water: Resource pack. Lancaster University, Lancaster, UK. More infor-mation can be found in our final report (Pullinger et al 2013) or email Dr Alison Browne [email protected]

Patterns of practice: A reflection on the development of quantitative/mixed methodologies capturing everday life related to water consumption in the UK.

There is a growing body of research arguing the relevance of practice approaches to understand resource consumption, and to highlight alternative pathways to sustainability. These practice approaches offer an alternative conceptualisation of demand and have been demonstrated largely by qualitative research, particularly in the work on water and energy consumption in the home. However, these historical narratives and qualitative research have not, to date, lead to the development of quantitative or mixed methodologies that could potentially reflect the diversity of performances of practice across populations in a more systematic way. This paper reflects, critically, on one such attempt to scale a practice-based perspective into a quantitative survey on water consumption and practice in homes in the south and South-East of England. The use of quantitative and mixed methodology has substantial potential – from translating practice-based research to policy; developing indictors to track patterns of practices as they change over time; and the exploration of methodologies that reflect the bundling and coordination of practices associated with water use inside and outside the home. The benefits and utility of such a methodological approach are highlighted as cautions and future research directions

Domestic heating behaviour and room temperatures: Empirical evidence from Scottish homes

In this paper, we describe patterns of residential heating based on data from 255 homes in and around Edinburgh, Scotland, UK, spanning August 2016 to June 2018. We describe: (i) the room temperatures achieved, (ii) the diurnal durations of heating use, and (iii) common diurnal patterns of heating behaviour. We investigate how these factors vary between weekdays and weekends, over the course of the year, by external temperature, and by room type. We compare these empirical findings with the simplifying assumptions about heating patterns found in the UK’s Standard Assessment Procedure (SAP), a widely-used building energy performance model. There are areas of concurrence and others of substantial difference with these model assumptions. Indoor achieved temperatures are substantially lower than SAP assumptions. The duration and timings of heating use vary substantially between homes and along lines of season and outdoor temperature, whereas the SAP model assumes no such variation. Little variation is found along the lines of weekday vs. weekend, whereas the SAP model assumes differences, or between living space and other rooms, consistent with the SAP. The results are relevant for those interested in how SAP assumptions regarding household heating behaviours and achieved indoor temperatures concur with empirical data

Exome sequencing in suspected monogenic dyslipidemias

Exome sequencing is a promising tool for gene mapping in Mendelian disorders. We used this technique in an attempt to identify novel genes underlying monogenic dyslipidemias