Facilitation of Male Sexual Behavior in Syrian Hamsters by the Combined Action of Dihydrotestosterone and Testosterone

Testosterone (T) controls male Syrian hamster sexual behavior, however, neither of T's primary metabolites, dihydrotestosterone (DHT) and estradiol (E(2)), even in highly supraphysiological doses, fully restores sexual behavior in castrated hamsters. DHT and T apparently interact with androgen receptors differentially to control male sexual behavior (MSB), but whether these two hormones act synergistically or antagonistically to control MSB has received scant experimental attention and is addressed in the present study.Sexually experienced male Syrian hamsters were gonadectomized and monitored 5 weeks later to confirm elimination of the ejaculatory reflex (week 0), at which time they received subcutaneous DHT-filled or empty capsules that remained in situ for the duration of the experiment. Daily injections of a physiological dose of 25 µg T or vehicle commenced two weeks after capsule implantation. MSB was tested 2, 4 and 5 weeks after T treatment began. DHT capsules were no more effective than control treatment for long-term restoration of ejaculation. Combined DHT + T treatment, however, restored the ejaculatory reflex more effectively than T alone, as evidenced by more rapid recovery of ejaculatory behavior, shorter ejaculation latencies, and a greater number of ejaculations in 30 minute tests.DHT and T administered together restored sexual behavior to pre-castration levels more rapidly than did T alone, whereas DHT and vehicle were largely ineffective. The additive actions of DHT and T on MSB are discussed in relation to different effects of these androgens on androgen receptors in the male hamster brain mating circuit

The naked truth:a comprehensive clarification and classification of current 'myths' in naked mole-rat biology

The naked mole-rat (Heterocephalus glaber) has fascinated zoologists for at least half a century. It has also generated considerable biomedical interest not only because of its extraordinary longevity, but also because of unusual protective features (e.g. its tolerance of variable oxygen availability), which may be pertinent to several human disease states, including ischemia/reperfusion injury and neurodegeneration. A recent article entitled 'Surprisingly long survival of premature conclusions about naked mole-rat biology' described 28 'myths' which, those authors claimed, are a 'perpetuation of beautiful, but falsified, hypotheses' and impede our understanding of this enigmatic mammal. Here, we re-examine each of these 'myths' based on evidence published in the scientific literature. Following Braude et al., we argue that these 'myths' fall into four main categories: (i) 'myths' that would be better described as oversimplifications, some of which persist solely in the popular press; (ii) 'myths' that are based on incomplete understanding, where more evidence is clearly needed; (iii) 'myths' where the accumulation of evidence over the years has led to a revision in interpretation, but where there is no significant disagreement among scientists currently working in the field; (iv) 'myths' where there is a genuine difference in opinion among active researchers, based on alternative interpretations of the available evidence. The term 'myth' is particularly inappropriate when applied to competing, evidence-based hypotheses, which form part of the normal evolution of scientific knowledge. Here, we provide a comprehensive critical review of naked mole-rat biology and attempt to clarify some of these misconceptions

Short Photoperiod Initiated During Adulthood Sustains Reproductive Function in Older Female Siberian Hamsters More Effectively Than Short Photoperiod Initiated Before Puberty1

Reproductive aging in female mammals is characterized by a progressive decline in fertility and fecundity. Many women delay their first full-term pregnancy until an age at which their reproductive potential has already declined. No treatment is presently available to delay the aging process. In a limited number of rodent species, caloric restriction sustained reproductive function in older females, and in most investigations, sexual maturation was delayed because caloric restriction was initiated at weaning. We have previously reported similar outcomes in female Siberian hamsters that were reared in short photoperiod (SP), which profoundly inhibits reproductive physiology. When compared to hamsters held in long photoperiod (LP), females reared in SP matured much later and had greater reproductive success at 9 mo of age. Herein, we determined if delayed onset of sexual maturation was necessary for SP to decelerate reproductive aging. We initiated a 6-mo period of SP before or after sexual maturation and measured the reproductive success of females at 12 mo of age. Maintenance of hamsters in SP beginning after puberty was associated with significantly greater litter success (77%) compared to imposition of SP before puberty (35%); the difference in weaning success was even greater (73% and 12%, respectively). Regardless of which SP regime was used, litter success of females exposed to SP was substantially greater than that of 12-mo-old females held continuously in LP (6%). The efficacy of SP in decelerating female reproductive aging is manifest at several life stages and is greater when treatment is initiated after rather than before puberty

T and DHT + T elevate blood T concentrations; DHT + T increases E₂ concentrations.

<p>Higher DHT concentrations are associated with increased ejaculatory behavior. (A) Testosterone, (B) estradiol, and (C) dihydrotestosterone concentrations. Bars designated with different letters differ significantly from one another N = 4–5 for each of the groups in panel A and 5–6 in panel B. C. N = 4 and 6 for hamsters that ejaculated or were non-ejaculators, respectively.</p

T and DHT + T treatments do not affect number of mounts but reduce mount latencies.

<p>Number of mounts (A) and mount latencies (B) preceding the first ejaculation. “a” significantly different from control group “b” significantly different from DHT + T group. Mount numbers did not differ significantly between groups over time.</p

DHT + T treatment reduces the number of intromissions preceding ejaculation.

<p>Number of intromissions (A) and intromission latencies (B), preceding the first ejaculation. * Significantly different from all other groups “a” significantly different from control group). “b” significantly different from DHT + T group.</p

DHT and T synergize to accelerate restoration of ejaculatory behavior.

<p>Percent of males displaying the ejaculatory reflex (A), number of ejaculations per 30 min test (B) and ejaculation latencies on the first ejaculatory series (C), during post-castration tests 2–5 wks after the onset of hormone or vehicle injections. * Significantly different from all other groups. “a” significantly different from the control group (p<0.05).</p

Socioecological Variables Predict Telomere Length in Wild Spotted Hyenas

Telomeres are regarded as important biomarkers of ageing and serve as useful tools in revealing how stress acts at the cellular level. However, the effects of social and ecological factors on telomere length remain poorly understood, particularly in free-ranging mammals. Here, we investigated the influences of within-group dominance rank and group membership on telomere length in wild adult spotted hyenas (Crocuta crocuta). We found large effects of both factors; high-ranking hyenas exhibited significantly greater mean telomere length than did subordinate animals, and group membership significantly predicted mean telomere length within high-ranking females. We further inquired whether prey availability mediates the observed effect of group membership on telomere length, but this hypothesis was not supported. Interestingly, adult telomere length was not predicted by age. Our work shows for the first time, to the best of our knowledge, the effects of social rank on telomere length in a wild mammal and enhances our understanding of how social and ecological variables may contribute to organismal senescence