53 research outputs found

    Application of 3D printing to prototype and develop novel plant tissue culture systems

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    Additional file 6. STL (StereoLithography) file was designed for an accessory lid without fan slot using SketchUp or Fusion 360 (Autodesk) software and the STL file was processed using MatterControl 3D printing software and exported as gcode files

    Immune dyscrasia in adult growth hormone deficiency: Evaluation of hemolytic complement activity (CH50) and IgG subclasses

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    CH50 is a screening assay for the activation of the classical complement pathway, the immunoglobulins-mediated one, activated in several inflammatory diseases. Adult growth hormone deficiency (aGHD) is recognized as a chronic inflammatory condition, although poorly evaluated under the profile of inflammatory biomarkers. The aim of this case-control observational study is to analyze CH50 and immunoglobulins G (IgG) subclasses production in aGHD, comparing this condition to healthy controls. 38 subjects were included and divided as follows: aGHD (n = 18, 6 females and 12 males); healthy controls (n = 20, 10 females and 10 males). GHD was diagnosed with dynamic test using Growth Hormone-Releasing Hormone (GHRH 50 \u3bcg i.v. + arginine 0,5 g/Kg), with a peak GH response < 9 \u3bcg/L when BMI was <30 kg/m2 or < 4 \u3bcg/L when BMI was >30 kg/m2. The two groups were evaluated for hormonal and metabolic parameters, CH50 and IgG subtypes. IgG1 and IgG2 were significantly higher in controls than in aGHD, while IgG3 and IgG4 showed a trend to higher levels in controls, although not significant. Furthermore, CH50 levels were significantly higher in aGHD. These data substantiate the hypothesis of a dyscrasia in IgG subclasses production in aGHD. As IgG levels decrease, CH50 levels do not

    Biofunctionalized Zinc Oxide Field Effect Transistors for Selective Sensing of Riboflavin with Current Modulation

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    Zinc oxide field effect transistors (ZnO-FET), covalently functionalized with single stranded DNA aptamers, provide a highly selective platform for label-free small molecule sensing. The nanostructured surface morphology of ZnO provides high sensitivity and room temperature deposition allows for a wide array of substrate types. Herein we demonstrate the selective detection of riboflavin down to the pM level in aqueous solution using the negative electrical current response of the ZnO-FET by covalently attaching a riboflavin binding aptamer to the surface. The response of the biofunctionalized ZnO-FET was tuned by attaching a redox tag (ferrocene) to the 3′ terminus of the aptamer, resulting in positive current modulation upon exposure to riboflavin down to pM levels

    USE OF ELISA WITH TT VIRUS(TTV) TO DETECT ANTI-TTV ANTIBODIES IN HUMAN SERA

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    Regeneration of shoots from immature and mature inflorescences of Cannabis sativa

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    Cannabis sativais usually clonally propagated from plants in the vegetative phase. However, phenotypic traits such as yield and chemical composition can only be assessed in unfertilized plants reaching the end of their life cycle and there are no peer-reviewed methods to propagate flowering plants. In this study, immature (three cultivars) and mature (one cultivar) floral explants were cultured on thidiazuron and shoot development was observed in both the immature and mature explants. This provides the first report of micropropagation from floral tissues in C. sativaand will enable plants to be clonally propagated up to the date of harvestThe accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
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