Agroforestry systems and innovation in extra-virgin olive oil chain in central Italy

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Risk of cancer after assisted reproduction: a review of the available evidences and guidance to fertility counselors

Infertile women requiring ovarian stimulation and assisted reproduction techniques (ART) are faced with difficult issues. The fear that using hormones could increase their risk of cancer is the most significant. One of the main challenges for assessing cancer risk after ART is the difficulty to separate it from the underlying condition of infertility per se. The delay or the inability to achieve a pregnancy is an important risk factor for breast, endometrial and ovarian cancer. We analyzed the current literature on the topic

Open and / or laparoscopic surgical treatment of liver hydatic cysts

Hydatid disease is a severe parasitic disease with a widely ranging distribution. In the human being the liver is the most frequent organ affected. 1 The treatment should be individualized to the morphology, size, number and location of the cysts, that is why a variety of surgical operations have been advocated from complete resection like total pericystectomy or partial hepatectomy to laparoscopy to a minimally invasive procedures like percutaneous aspiration of cysts to conservative drug therapy. 3-4 This study compares laparoscopic versus open management of the hydatid cyst of liver the surgical approach to liver echinococcosis is still a controversial issue and shows our results of surgical treatment of liver hydatid cysts during a 3-years period

Social and economical evaluation of innovative alley coppice systems mixing timber trees with bioenergy wood crops in agroforestry systems

PosterAlley coppice (AC) is an innovative agroforestry system where high value timber trees in lines are intercropped with bioenergy short rotation coppice (SRC). AC potentially provides several production and environmental benefits, although so far these are poorly investigated. Thus, research on farmer interest in AC systems, and preliminary economic evaluations, have been made on the AgroCop European project (www.agrocop.com). A questionnaire was conducted with farmers in Italy to identify and assess their interests and concerns regarding AC systems. Farmers appeared to be familiar with managing forest plantations as species mixtures and the value of fuelwood. However, the value of the timber and biomass produced during the rotation appeared to be farmers’ main source of uncertainty, with no clear idea of what demand there would be for both wood products, and they assigned a higher rank to the importance of environmental benefits (biodiversity, landscape, soil fertility improvement). This indicated that there was a need to study the economic performance of the AC system developing two tools: a simplified economic calculator for stakeholders, that can estimate and compare net present value (NPV) of different AC systems, and a more complex model, adapting FarmSAFE. Biophysical data was generated using LERSAFE. Wood chips from the SRC component were found to have a low market value, and AC profitability depends mostly on the timber trees, with the essential need to optimize revenue from the valuable timber, especially by maximizing its quality. Therefore, the SRC component has an important role to play in increasing the quality of the valuable timber by improving timber tree form, reducing the costs of management operations and for giving a revenue during the growth of standard trees. AC could be an innovative option for timber and bioenergy production, integrating some of the advantages that are characteristic of agroforestry systems into mixed tree plantations

Host-Specific NS5 Ubiquitination Determines Yellow Fever Virus Tropism

The recent yellow fever virus (YFV) epidemic in Brazil in 2017 and Zika virus (ZIKV) epidemic in 2015 serve to remind us of the importance of flaviviruses as emerging human pathogens. With the current global flavivirus threat, there is an urgent need for antivirals and vaccines to curb the spread of these viruses. However, the lack of suitable animal models limits the research questions that can be answered. A common trait of all flaviviruses studied thus far is their ability to antagonize interferon (IFN) signaling so as to enhance viral replication and dissemination. Previously, we reported that YFV NS5 requires the presence of type I IFN (IFN-α/β) for its engagement with human signal transducer and activator of transcription 2 (hSTAT2). In this manuscript, we report that like the NS5 proteins of ZIKV and dengue virus (DENV), YFV NS5 protein is able to bind hSTAT2 but not murine STAT2 (mSTAT2). Contrary to what has been demonstrated with ZIKV NS5 and DENV NS5, replacing mSTAT2 with hSTAT2 cannot rescue the YFV NS5-STAT2 interaction, as YFV NS5 is also unable to interact with hSTAT2 in murine cells. We show that the IFN-α/β-dependent ubiquitination of YFV NS5 that is required for STAT2 binding in human cells is absent in murine cells. In addition, we demonstrate that mSTAT2 restricts YFV replication in vivo These data serve as further impetus for the development of an immunocompetent mouse model that can serve as a disease model for multiple flaviviruses.IMPORTANCE Flaviviruses such as yellow fever virus (YFV), Zika virus (ZIKV), and dengue virus (DENV) are important human pathogens. A common flavivirus trait is the antagonism of interferon (IFN) signaling to enhance viral replication and spread. We report that like ZIKV NS5 and DENV NS5, YFV NS5 binds human STAT2 (hSTAT2) but not mouse STAT2 (mSTAT2), a type I IFN (IFN-α/β) pathway component. Additionally, we show that contrary to what has been demonstrated with ZIKV NS5 and DENV NS5, YFV NS5 is unable to interact with hSTAT2 in murine cells. We demonstrate that mSTAT2 restricts YFV replication in mice and that this correlates with a lack of IFN-α/β-induced YFV NS5 ubiquitination in murine cells. The lack of suitable animal models limits flavivirus pathogenesis, vaccine, and drug research. These data serve as further impetus for the development of an immunocompetent mouse model that can serve as a disease model for multiple flaviviruses

The role of very low calorie ketogenic diet in sympathetic activation through cortisol secretion in male obese population

Adipose tissue is considered an endocrine organ, and its excess compromises the immune response and metabolism of hormones and nutrients. Furthermore, the accumulation of visceral fat helps to increase the synthesis of cortisol. The hypothalamus-pituitary-adrenal (HPA) axis is a neuroendocrine system involved in maintaining homeostasis in humans under physiological conditions and stress, and cortisol is the main hormone of the HPA axis. It is known that a stress-induced diet and cortisol reactivity to acute stress factors may be related to dietary behavior. In obesity, to reduce visceral adipose tissue, caloric restriction is a valid strategy. In light of this fact, the aim of this study was to assess the effects of a commercial dietary ketosis program for weight loss on the sympathetic nervous system and HPA axis, through evaluation of salivary cortisol and GSR levels. Thirty obese subjects were recruited and assessed before and after 8 weeks of Very Low Calorie Ketogenic Diet (VLCKD) intervention to evaluate body composition and biochemical parameters. Salivary cortisol levels and GSR significantly decreased after dietary treatment; in addition, body composition and biochemical features were ameliorated. The VLCKD had a short-term positive effect on the SNS and HPA axes regulating salivary cortisol levels. Finally, the effects of the VLCKD on the SNS and HPA axis may lead to more individualized treatment strategies that integrate obesity and stress and support the usefulness of such therapeutic interventions in promoting the reduction of the individual disease burden

The role of rural development policy in supporting agroforestry systems in EU

PresentationA financial support (Measure 222) was introduced in the EU Rural Development Programmes (RDPs) 2007-2013 aimed at promoting the establishment of agroforestry systems on arable lands. The objectives of the paper are to: i) assess the implementation rate of the Measure 222 in EU27 during the period 2007-2013; ii) identify the main reasons and constraints that affected the farmers’ interest in the Measure 222, iii) highlight the perspectives in the next RDPs 2014-2020. The data on RDPs monitoring were obtained from the European Network for Rural Development (http://enrd.ec.europa.eu) and analyzed. The study compared the financial resources allocated to implement the Measure 222 with: i) the resources allocated to implement other forestry Measures; ii) the effective expenditures invested in establishing new agroforestry systems. The output indicators (number of beneficiaries and hectares under new agroforestry systems) were also analyzed in relation to their expected target. The Measure 222 was poorly applied across EU27: only few EU Regions have allocated resources to the Measure 222 and only 3.4% of these resources has been effectively invested to create new agroforestry systems on arable lands. Moreover, only 2.3% of the expected beneficiaries has been targeted and 2.1% of the expected hectares has been realized. The main constraints that have hampered the success of the Measure 222 in EU27 are reported. The new Regulation (EU Reg. 1305/2013) in support of rural development 2014-2020 states that: i) agroforestry systems comprise the combination between forestry plantations and agriculture on the same land; ii) grants should cover the establishment costs (up to 80% of the expenses) and the maintenance costs with an annual premium for 5 years; iii) beneficiaries should be not limited to farmers but may include also Municipalities and Associations. This new grant scheme should raise farmers and land owners interest in agroforestry systems

First-in-human pharmacokinetics of tamoxifen and its metabolites in the milk of a lactating mother. A case study

Background Breast cancer represents the most frequent neoplasm diagnosed in women of childbearing age. When the tumour is oestrogen receptor-positive, tamoxifen is among the recommended endocrine treatments. Lactating women are advised not to breastfeed while receiving tamoxifen. However, information about tamoxifen transfer into breast milk is lacking. Methods We measured the concentration of tamoxifen and its metabolites by liquid chromatography-tandem mass spectrometry in the milk of a nursing mother that was treated for pregnancy-associated breast cancer diagnosed a few months after delivery. She was advised not to breastfeed her child and she collected milk samples for 23 days while the baby was fed with formula. Results Tamoxifen concentrations in milk increased reaching a maximum of 214 nM. The two active metabolitesZ-4-hydroxy-tamoxifen and Z-endoxifen, could not be quantified in milk the first days after tamoxifen intake, but increased over time and reached clinically significant levels after day 18. Conclusion This study demonstrates for the first time in human that tamoxifen and its metabolites transfer into milk. Since tamoxifen has a complete oral bioavailability, a long half-life (>7 days) and may interfere with the normal development of the infant, mothers should not breastfeed during tamoxifen treatment

Farmers’ reasoning behind the uptake of agroforestry practices: evidence from multiple case-studies across Europe

Potential benefits and costs of agroforestry practices have been analysed by experts, but few studies have captured farmers’ perspectives on why agroforestry might be adopted on a European scale. This study provides answers to this question, through an analysis of 183 farmer interviews in 14 case study systems in eight European countries. The study systems included high natural and cultural value agroforestry systems, silvoarable systems, high value tree systems, and silvopasture systems, as well as systems where no agroforestry practices were occurring. A mixed method approach combining quantitative and qualitative approaches was taken throughout the interviews. Narrative thematic data analysis was performed. Data collection proceeded until no new themes emerged. Within a given case study, i.e. the different systems in different European regions, this sampling was performed both for farmers who practice agroforestry and farmers who did not. Results point to a great diversity of agroforestry practices, although many of the farmers are not aware of the term or concept of agroforestry, despite implementing the practice in their own farms. While only a few farmers mentioned eligibility for direct payments in the CAP as the main reason to remove trees from their land, to avoid the reduction of the funded area, the tradition in the family or the region, learning from others, and increasing the diversification of products play the most important role in adopting or not agroforestry systems