Debates: Does Information Theory Provide a New Paradigm for Earth Science? Emerging Concepts and Pathways of Information Physics

Entropy and Information are key concepts not only in Information Theory but also in Physics: historically in the fields of Thermodynamics, Statistical and Analytical Mechanics, and, more recently, in the field of Information Physics. In this paper we argue that Information Physics reconciles and generalizes statistical, geometric, and mechanistic views on information. We start by demonstrating how the use and interpretation of Entropy and Information coincide in Information Theory, Statistical Thermodynamics, and Analytical Mechanics, and how this can be taken advantage of when addressing Earth Science problems in general and hydrological problems in particular. In the second part we discuss how Information Physics provides ways to quantify Information and Entropy from fundamental physical principles. This extends their use to cases where the preconditions to calculate Entropy in the classical manner as an aggregate statistical measure are not met. Indeed, these preconditions are rarely met in the Earth Sciences due either to limited observations or the far-from-equilibrium nature of evolving systems. Information Physics therefore offers new opportunities for improving the treatment of Earth Science problems.info:eu-repo/semantics/publishedVersio

Design and analysis of a database to evaluate children’s reading aloud performance

To evaluate the reading performance of children, human assessment is usually involved, where a teacher or tutor has to take time to individually estimate the performance in terms of fluency (speed, accuracy and expression). Automatic estimation of reading ability can be an important alternative or complement to the usual methods, and can improve other applications such as e-learning. Techniques must be developed to analyse audio recordings of read utterances by children and detect the deviations from the intended correct reading i.e. disfluencies. For that goal, a database of 284 European Portuguese children from 6 to 10 years old (1st–4th grades) reading aloud amounting to 20 h was collected in private and public Portuguese schools. This paper describes the design of the reading tasks as well as the data collection procedure. The presence of different types of disfluencies is analysed as well as reading performance compared to known curricular goals.info:eu-repo/semantics/acceptedVersio

Monosomal karyotype (MK) in myeloid malignancies

Review on Monosomal karyotype (MK) in myeloid malignancies, with data on clinics, and the genes involved

Impairment of the erythrocyte membrane fluidity in survivors of acute myocardial infarction : a prospective study

© 1999 – IOS Press. All rights reservedErythrocytes have to constantly adapt themselves to the varying circulatory system shear stress forces and capillaries diameter. Membrane lipid and protein content have an important role in determining the erythrocyte shape and are main determinants of the membrane solid and fluid behavior which enables the erythrocyte to respond to the outer environment modifications. Membrane fluidity is an inverse index of membrane microviscosity. The aim of the present work is to evaluate prospectively in three periods of time (discharge, after 6 months and one year later) in survivors of an acute myocardial infarction (AMI) the erythrocyte membrane fluidity (outer and inner bilayer) and establish a relation with the cardiovascular events or need of coronary revascularization during a two year clinical follow up. Sixty survivors of acute myocardial infarction were recruited during 1994–96 and were prospectively studied in three periods (discharge, 6 months and after one year), and were compared with a control group (n = 36). Membrane lipid fluidity was determined by means of fluorescence polarization with two probes: 1,6-diphenyl-1,2,5-hexatriene (DPH) and 1,4-trimethylamine 6-phenyl hexa-1,3,5-triene (TMA-DPH), for the characterisation of the hydrophobic and external polar region, respectively. The hydrophobic region was more rigidified (p < 0:01) in the erythrocytes from AMI patients, in relation to the control group. During the time of the study there was a progressive erythrocyte membrane rigidification (DPH p < 0:001; TMA-DPH p < 0:001). We found no relation between erythrocyte membrane fluidity and the coronary risk factors, cardiovascular events or the need of coronary revascularization during the clinical follow-up. In conclusion, after the myocardial infarction erythrocyte membrane of AMI survivors becomes more rigid with time, which could contribute to the decreased erythrocyte deformability and the increased blood viscosity previously described in this group of patients

Study of the magnetic disaccommodation in La doped YIG

The relaxation of the initial magnetic permeability of La doped yttrium iron garnet (YIG) samples with nominal composition Y3-xLaxFe5O12 (0< x <0.6) is analysed in this work. The results show a very different behaviour depending on the sintering atmosphere used for the fabrication of the samples. Concretely, for the samples sintered in air, it just have been detected the usual relaxation peak found in YIG at 130 K. However, a new relaxation peak appears around room temperature for the samples sintered in CO2 atmosphere when the La content is at least of 0.3. These results have been interpreted in terms of the formation of a secondary perovskite phase when the La solubility limit is reached. (© 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

A Phylogenomic Approach for the Analysis of Colistin Resistance-Associated Genes in Klebsiella Pneumoniae, its Mutational Diversity and Implications for Phenotypic Resistance

The emergence of carbapenemase-producing Klebsiella pneumoniae strains has triggered the use of old antibiotics such as colistin. This is driving the emergence of colistin resistance in multidrug-resistant strains that underlie life-threatening infections. This study analyses the mutational diversity of 22 genes associated with colistin resistance in 140 K. pneumoniae clinical isolates integrated in a high-resolution phylogenetic scenario. Colistin susceptibility was accessed by broth microdilution. A total of 98 isolates were susceptible and 16 were resistant, 10 of which were carbapenemase producers. Across the 22 genes examined, 171 non-synonymous mutations and 9 mutations associated with promoter regions were found. Eighty-five isolates had a truncation and/or deletion in at least one of the 22 genes. However, only seven mutations, the complete deletion of mgrB or insertion sequence (IS)-mediated disruption, were exclusively observed in resistant isolates. Four of these (mgrBIle13fs, pmrBGly207Asp, phoQHis339Asp and ramAIle28Met) comprised novel mutations that are potentially involved in colistin resistance. One strain bore a ISEcp1-blaCTX-M-15::mgrB disruption, underlying co-resistance to third-generation cephalosporins and colistin. Moreover, the high-resolution phylogenetic context shows that most of the mutational diversity spans multiple phylogenetic clades, and most of the mutations previously associated with colistin resistance are clade-associated and present in susceptible isolates, showing no correlation with colistin resistance. In conclusion, the present study provides relevant data on the genetic background of genes involved with colistin resistance deeply rooted across monophyletic groups and provides a better understanding of the genes and mutations involved in colistin resistance.info:eu-repo/semantics/publishedVersio

An update on wastewater multi-resistant bacteria : identification of clinical pathogens such as Escherichia coli O25b:H4-B2-ST131-producing CTX-M-15 ESBL and KPC-3 Carbapenemase-Producing Klebsiella oxytoca

© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Wastewater treatment plants (WWTPs) are significant reservoirs of bacterial resistance. This work aims to identify the determinants of resistance produced by Gram-negative bacteria in the influent and effluent of two WWTPs in Portugal. A total of 96 wastewater samples were obtained between 2016 and 2019. The numbers of total aerobic and fecal contamination bacteria were evaluated, and genomic features were searched by polymerase chain reaction (PCR) and Next-Generation Sequencing (NGS). Enterobacteriaceae corresponded to 78.6% (n = 161) of the 205 isolates identified by 16sRNA. The most frequent isolates were Escherichia spp. (57.1%, n = 117), followed by Aeromonas spp. (16.1%, n = 33) and Klebsiella spp. (12.7%, n = 26). The remaining 29 isolates (14.1%) were distributed across 10 different genera. Among the 183 resistant genes detected, 54 isolates produced extended spectrum β-lactamases (ESBL), of which blaCTX-M-15 was predominant (37 isolates; 68.5%). A KPC-3 carbapenemase-producing K. oxytoca was identified (n = 1), with blaKPC-3 included in a transposon Tn4401 isoform b. A higher number of virulence genes (VG) (19 genes) was found in the E. coli 5301 (O25b-ST131-B2) isolate compared with a commensal E. coli 5281 (O25b-ST410-A) (six genes). Both shared five VG [Enterobactin; Aerobactin, CFA/1 (clade α); Type1 (clade γ1); Type IV]. In conclusion, this work highlights the role of relevant clinical bacteria in WWTPs, such as KPC-3-producing K. oxytoca, and, for the first time, a CTX-M-15-producing Ochromobactrum intermedium, a human opportunistic pathogen, and a SED-1-producing Citrobacter farmeri, an uncommon CTX-M-type extended-spectrum beta-lactamase.This work was supported by the European Union LIFE Programme under Grant Agreement LIFE14 ENV/PT/000739—LIFE Impetus (https://life-impetus.eu/), accessed on 1 February 2021.info:eu-repo/semantics/publishedVersio

Quality and Safety Indicators in Anesthesia

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CRISPR-Cas9 correction of OPA1 c.1334G>A: p.R445H restores mitochondrial homeostasis in dominant optic atrophy patient-derived iPSCs.

Autosomal dominant optic atrophy (DOA) is the most common inherited optic neuropathy in the United Kingdom. DOA has an insidious onset in early childhood, typically presenting with bilateral, central visual loss caused by the preferential loss of retinal ganglion cells. 60%-70% of genetically confirmed DOA cases are associated with variants in OPA1, a ubiquitously expressed GTPase that regulates mitochondrial homeostasis through coordination of inner membrane fusion, maintenance of cristae structure, and regulation of bioenergetic output. Whether genetic correction of OPA1 pathogenic variants can alleviate disease-associated phenotypes remains unknown. Here, we demonstrate generation of patient-derived OPA1 c.1334G>A: p.R445H mutant induced pluripotent stem cells (iPSCs), followed by correction of OPA1 through CRISPR-Cas9-guided homology-directed repair (HDR) and evaluate the effect of OPA1 correction on mitochondrial homeostasis. CRISPR-Cas9 gene editing demonstrated an efficient method of OPA1 correction, with successful gene correction in 57% of isolated iPSCs. Correction of OPA1 restored mitochondrial homeostasis, re-establishing the mitochondrial network and basal respiration and ATP production levels. In addition, correction of OPA1 re-established the levels of wild-type (WT) mitochondrial DNA (mtDNA) and reduced susceptibility to apoptotic stimuli. These data demonstrate that nuclear gene correction can restore mitochondrial homeostasis and improve mtDNA integrity in DOA patient-derived cells carrying an OPA1 variant