Nesting Behavior of Palila, as Assessed from Video Recordings

We quantified nesting behavior of Palila (Loxioides bailleui), an endangered Hawaiian honeycreeper, by recording at nests during three breeding seasons using a black-and-white video camera connected to a videocassette recorder. A total of seven nests was observed. We measured the following factors for daylight hours: percentage of time the female was on the nest (attendance), length of attendance bouts by the female, length of nest recesses, and adult provisioning rates. Comparisons were made between three stages of the 40-day nesting cycle: incubation (day 1–day 16), early nestling stage (day 17–day 30 [i.e., nestlings ≤ 14 days old]), and late nestling stage (day 31–day 40 [i.e., nestlings \u3e 14 days old]). Of seven nests observed, four fledged at least one nestling and three failed. One of these failed nests was filmed being depredated by a feral cat (Felis catus). Female nest attendance was near 82% during the incubation stage and decreased to 21% as nestlings aged. We did not detect a difference in attendance bout length between stages of the nesting cycle. Mean length of nest recesses increased from 4.5 min during the incubation stage to over 45 min during the late nestling stage. Mean number of nest recesses per hour ranged from 1.6 to 2.0. Food was delivered to nestlings by adults an average of 1.8 times per hour for the early nestling stage and 1.5 times per hour during the late nestling stage and did not change over time. Characterization of parental behavior by video had similarities to but also key differences from findings taken from blind observations. Results from this study will facilitate greater understanding of Palila reproductive strategies

Recurrence of Cervical Cancer in Mice after Selective Estrogen Receptor Modulator Therapy

Estrogen and its nuclear receptor, estrogen receptor ?, are necessary cofactors in the initiation and multistage progression of carcinogenesis in the K14E6/E7 transgenic mouse model of human papillomavirus–associated cervical cancer. Recently, our laboratory reported that raloxifene, a selective estrogen receptor modulator, promoted regression of high-grade dysplasia and cancer that arose in the cervix of K14E6/E7 transgenic mice treated long-term with estrogen. Herein, we evaluated the recurrence of cervical cancer after raloxifene therapy in our preclinical model of human papillomavirus–associated cervical carcinogenesis. We observed recurrence of cervical cancer in mice re-exposed to estrogen after raloxifene treatment, despite evidence suggesting the antagonistic effects of raloxifene persisted in the reproductive tract after treatment had ceased. We also observed recurrence of neoplastic disease in mice that were not retreated with exogenous estrogen, although the severity of disease was less. Recurrent neoplastic disease and cancers retained functional estrogen receptor ? and responded to retreatment with raloxifene. Moreover, continuous treatment of mice with raloxifene prevented the emergence of recurrent disease seen in mice in which raloxifene was discontinued. These data suggest that cervical cancer cells are not completely eradicated by raloxifene and rapidly expand if raloxifene treatment is ceased. These findings indicate that a prolonged treatment period with raloxifene might be required to prevent recurrence of neoplastic disease and lower reproductive tract cancers

The multikinase inhibitor RXDX-105 is effective against neuroblastoma in vitro and in vivo.

Neuroblastoma is the most common extracranial solid tumor of childhood and accounts for 15% of all pediatric cancer-related deaths. New therapies are needed to improve outcomes for children with high-risk and relapsed tumors. Inhibitors of the RET kinase and the RAS-MAPK pathway have previously been shown to be effective against neuroblastoma, suggesting that combined inhibition may have increased efficacy. RXDX-105 is a small molecule inhibitor of multiple kinases, including the RET and BRAF kinases. We found that treatment of neuroblastoma cells with RXDX-105 resulted in a significant decrease in cell viability and proliferation in vitro and in tumor growth and tumor vascularity in vivo. Treatment with RXDX-105 inhibited RET phosphorylation and phosphorylation of the MEK and ERK kinases in neuroblastoma cells and xenograft tumors, and RXDX-105 treatment induced both apoptosis and cell cycle arrest. RXDX-105 also showed enhanced efficacy in combination with 13-cis-retinoic acid, which is currently a component of maintenance therapy for children with high-risk neuroblastoma. Our results demonstrate that RXDX-105 shows promise as a novel therapeutic agent for children with high-risk and relapsed neuroblastoma

Managing Performance [in Child Welfare Supervision]

One of the primary roles of a supervisor is to manage worker performance. Performance management is the continuous process of identifying, measuring, and developing the performance of individuals and teams and aligning performance with the strategic goals of the organization (Aguinis, 2007, p. 2). Supervisors must regularly assess current performance levels and take steps to improve performance in a way that is congruent with agency goals. The ultimate goal is to achieve agency objectives through individual and team performance. To effectively manage performance, supervisors must know what the performance expectations are for workers and clearly communicate these expectations to workers. They must regularly monitor and document performance while taking steps to facilitate and improve performance. In the event of unsatisfactory performance patterns, supervisors must analyze and address performance problems, sometimes using disciplinary measures. Finally, formalized performance management systems may include standardized performance evaluations, which supervisors will be expected to complete. In carrying out these multiple responsibilities, supervisors must be aware of and operate within legal guidelines for performance management. Child welfare supervisors with case management experience will find themselves well prepared to manage worker performance. The responsibilities are not unlike those required to help families ensure the safety and well-being of their children. Case management skills related to assessment, collaborative planning, goal setting, problem solving, progress evaluation, and documentation are valuable assets for evaluating and facilitating worker performance. Child welfare supervisors should capitalize on these strengths as they seek to help workers succeed

Managing Performance [in Child Welfare Supervision]

One of the primary roles of a supervisor is to manage worker performance. Performance management is the continuous process of identifying, measuring, and developing the performance of individuals and teams and aligning performance with the strategic goals of the organization (Aguinis, 2007, p. 2). Supervisors must regularly assess current performance levels and take steps to improve performance in a way that is congruent with agency goals. The ultimate goal is to achieve agency objectives through individual and team performance. To effectively manage performance, supervisors must know what the performance expectations are for workers and clearly communicate these expectations to workers. They must regularly monitor and document performance while taking steps to facilitate and improve performance. In the event of unsatisfactory performance patterns, supervisors must analyze and address performance problems, sometimes using disciplinary measures. Finally, formalized performance management systems may include standardized performance evaluations, which supervisors will be expected to complete. In carrying out these multiple responsibilities, supervisors must be aware of and operate within legal guidelines for performance management. Child welfare supervisors with case management experience will find themselves well prepared to manage worker performance. The responsibilities are not unlike those required to help families ensure the safety and well-being of their children. Case management skills related to assessment, collaborative planning, goal setting, problem solving, progress evaluation, and documentation are valuable assets for evaluating and facilitating worker performance. Child welfare supervisors should capitalize on these strengths as they seek to help workers succeed

Precision cancer monitoring using a novel, fully integrated, microfluidic array partitioning digital PCR platform.

A novel digital PCR (dPCR) platform combining off-the-shelf reagents, a micro-molded plastic microfluidic consumable with a fully integrated single dPCR instrument was developed to address the needs for routine clinical diagnostics. This new platform offers a simplified workflow that enables: rapid time-to-answer; low potential for cross contamination; minimal sample waste; all within a single integrated instrument. Here we showcase the capability of this fully integrated platform to detect and quantify non-small cell lung carcinoma (NSCLC) rare genetic mutants (EGFR T790M) with precision cell-free DNA (cfDNA) standards. Next, we validated the platform with an established chronic myeloid leukemia (CML) fusion gene (BCR-ABL1) assay down to 0.01% mutant allele frequency to highlight the platform's utility for precision cancer monitoring. Thirdly, using a juvenile myelomonocytic leukemia (JMML) patient-specific assay we demonstrate the ability to precisely track an individual cancer patient's response to therapy and show the patient's achievement of complete molecular remission. These three applications highlight the flexibility and utility of this novel fully integrated dPCR platform that has the potential to transform personalized medicine for cancer recurrence monitoring