Patterns of cadmium accumulation in selected tissues of the catfish Clarias batrachus (Linn.) exposed to sublethal concentration of cadmium chloride

The pattern of accumulation of cadmium and its affinity to selected tissues - gills, kidneys, liver, skin and muscle - of Clarias batrachus exposed to sublethal concentrations (7 ppm) of cadmium chloride was investigated. These tissues showed significant variations in the patterns of accumulation of cadmium. The rate of accumulation varies from tissue to tissue as well as at various exposure periods. The mean rate of accumulation after 60 days of exposure was in the order gills > kidneys > liver > skin > muscle. The difference in the rate of accumulation may be attributed to the proximity of the tissues to the toxicant medium, physiological state of the tissues and presence of ligands in the tissues having an affinity to cadmium

MULTIPLE QUANTUM WALKERS ON THE LINE USING HYBRID COINS: A POSSIBLE TOOL FOR QUANTUM SEARCH

In this paper discrete quantum walks with different coins used for odd and even time steps are studied. These coins are called hybrid. The calculation results are compared with the most frequently used coin, the Hadamard transform. Furthermore, quantum walks on the line which involve two or more quantum walkers with hybrid coins are studied. Quantum walks with entangled walkers and hybrid coins are also studied. The results of these calculations show that the proposed types of quantum walks can be used for quantum search, because the walker can be directed towards preferred directions and can also be confined in certain segments of the line

Gene dose influences cellular and calcium channel dysregulation in heterozygous and homozygous T4826I-RYR1 malignant hyperthermia-susceptible muscle

Malignant hyperthermia susceptibility (MHS) is primarily conferred by mutations within ryanodine receptor type 1 (RYR1). Here we address how the MHS mutation T4826I within the S4-S5 linker influences excitation-contraction coupling and resting myoplasmi

'Special K' and a loss of cell-to-cell adhesion in proximal tubule-derived epithelial cells: modulation of the adherens junction complex by ketamine

Ketamine, a mild hallucinogenic class C drug, is the fastest growing ‘party drug’ used by 16–24 year olds in the UK. As the recreational use of Ketamine increases we are beginning to see the signs of major renal and bladder complications. To date however, we know nothing of a role for Ketamine in modulating both structure and function of the human renal proximal tubule. In the current study we have used an established model cell line for human epithelial cells of the proximal tubule (HK2) to demonstrate that Ketamine evokes early changes in expression of proteins central to the adherens junction complex. Furthermore we use AFM single-cell force spectroscopy to assess if these changes functionally uncouple cells of the proximal tubule ahead of any overt loss in epithelial cell function. Our data suggests that Ketamine (24–48 hrs) produces gross changes in cell morphology and cytoskeletal architecture towards a fibrotic phenotype. These physical changes matched the concentration-dependent (0.1–1 mg/mL) cytotoxic effect of Ketamine and reflect a loss in expression of the key adherens junction proteins epithelial (E)- and neural (N)-cadherin and β-catenin. Down-regulation of protein expression does not involve the pro-fibrotic cytokine TGFβ, nor is it regulated by the usual increase in expression of Slug or Snail, the transcriptional regulators for E-cadherin. However, the loss in E-cadherin can be partially rescued pharmacologically by blocking p38 MAPK using SB203580. These data provide compelling evidence that Ketamine alters epithelial cell-to-cell adhesion and cell-coupling in the proximal kidney via a non-classical pro-fibrotic mechanism and the data provides the first indication that this illicit substance can have major implications on renal function. Understanding Ketamine-induced renal pathology may identify targets for future therapeutic intervention

Role of surface roughness in hard x-ray emission from femtosecond laser produced copper plasmas

The hard x-ray emission in the energy range of 30-300 keV from copper plasmas produced by 100 fs, 806 nm laser pulses at intensities in the range of 10$^{15}-10^{16}$ W cm$^{-2}$ is investigated. We demonstrate that surface roughness of the targets overrides the role of polarization state in the coupling of light to the plasma. We further show that surface roughness has a significant role in enhancing the x-ray emission in the above mentioned energy range.Comment: 5 pages, 4 figures, to appear in Phys. Rev.

ATOMS : ALMA three-millimeter observations of massive star-forming regions - XII: Fragmentation and multiscale gas kinematics in protoclusters G12.42+0.50 and G19.88-0.53

We present new continuum and molecular line data from the ALMA Three-millimeter Observations of Massive Star-forming regions (ATOMS) survey for the two protoclusters, G12.42+0.50 and G19.88-0.53. The 3 mm continuum maps reveal seven cores in each of the two globally contracting protoclusters. These cores satisfy the radius-mass relation and the surface mass density criteria for high-mass star formation. Similar to their natal clumps, the virial analysis of the cores suggests that they are undergoing gravitational collapse (alpha(vir) < < 2). The clump to core scale fragmentation is investigated and the derived core masses and separations are found to be consistent with thermal Jeans fragmentation. We detect large-scale filamentary structures with velocity gradients and multiple outflows in both regions. Dendrogram analysis of the (HCO+)-C-13 map identities several branch and leaf structures with sizes similar to 0.1 and 0.03 pc, respectively. The supersonic gas motion displayed by the branch structures is in agreement with the Larson power law indicating that the gas kinematics at this spatial scale is driven by turbulence. The transition to transonic/subsonic gas motion is seen to occur at spatial scales of similar to 0.1 pc indicating the dissipation of turbulence. In agreement with this, the leaf structures reveal gas motions that deviate from the slope of Larson's law. From the largescale converging filaments to the collapsing cores, the gas dynamics in G12.42+0.50 and G19.88-0.53 show scale-dependent dominance of turbulence and gravity and the combination of these two driving mechanisms needs to be invoked to explain massive star formation in the protoclusters.Peer reviewe

Can a quality improvement project impact maternal and child health outcomes at scale in northern Ghana?

Abstract Background Quality improvement (QI) interventions are becoming more common in low- and middle-income countries, yet few studies have presented impact evaluations of these approaches. In this paper, we present an impact evaluation of a scale-up phase of ‘Project Fives Alive!’, a QI intervention in Ghana that aims to improve maternal and child health outcomes. ‘Project Fives Alive!’ employed a QI methodology to recognize barriers to care-seeking and care provision at the facility level and then to identify, test and implement simple and low-cost local solutions that address the barriers. Methods A quasi-experimental design, multivariable interrupted time series analysis, with data coming from 744 health facilities and controlling for potential confounding factors, was used to study the effect of the project. The key independent variables were the change categories (interventions implemented) and implementation phase – Wave 2a (early phase) versus Wave 2b (later phase). The outcomes studied were early antenatal care (ANC), skilled delivery, facility-level under-five mortality and attendance of underweight infants at child welfare clinics. We stratified the analysis by facility type, namely health posts, health centres and hospitals. Results Several of the specific change categories were significantly associated with improved outcomes. For example, three of five change categories (early ANC, four or more ANC visits and skilled delivery/immediate postnatal care (PNC)) for health posts and two of five change categories (health education and triage) for hospitals were associated with increased skilled delivery. These change categories were associated with increases in skilled delivery varying from 28% to 58%. PNC changes for health posts and health centres were associated with greater attendance of underweight infants at child welfare clinics. The triage change category was associated with increased early antenatal care in hospitals. Intensity, the number of change categories tested, was associated with increased skilled delivery in health centres and reduced under-five mortality in hospitals. Conclusions Using an innovative evaluation technique we determined that ‘Project Fives Alive!’ demonstrated impact at scale for the outcomes studied. The QI approach used by this project should be considered by other low- and middle-income countries in their efforts to improve maternal and child health