Vegetative growth response of beets and lettuce to stored human urine

ArticleIn this work, we present the experimental results of the effect of stored human urine (SHU) on the growth of beets (Beta vulgaris L) and lettuce (Lactuca sativaL). We apply different amounts of SHU according to the recommended dose of nitrogen, considering soil from farmland and vermiculite as substrates. The last allows us to determine with high precision the isolated effect of SHU over the vegetative development of beet plants, without considering other nutrients present in common soils. Experimental results demonstrate that the application of SHU has no significant effects on lettuce vegetative growth under our soil conditions. In contrast, SHU can be used successfully as a fertilizer of beets. The optimum dose was found at 120kgN ha-1and resulted in average dry weight of 125g. However, if the dose exceeds the optimum levels, the growth of the plant is inhibited. Beets fertilized with SHU does not pose any hygienic risk for human consumption. Our findings represent a promising alternative to propose expanding the use of SHU as fertilizer in medium-sized greenhouses and to provide benefits to families in rural areas, with little or no available water supplies

The utility of Next Generation Sequencing for molecular diagnostics in Rett syndrome

Rett syndrome (RTT) is an early-onset neurodevelopmental disorder that almost exclusively affects girls and is totally disabling. Three genes have been identified that cause RTT: MECP2, CDKL5 and FOXG1. However, the etiology of some of RTT patients still remains unknown. Recently, next generation sequencing (NGS) has promoted genetic diagnoses because of the quickness and affordability of the method. To evaluate the usefulness of NGS in genetic diagnosis, we present the genetic study of RTT-like patients using different techniques based on this technology. We studied 1577 patients with RTT-like clinical diagnoses and reviewed patients who were previously studied and thought to have RTT genes by Sanger sequencing. Genetically, 477 of 1577 patients with a RTT-like suspicion have been diagnosed. Positive results were found in 30% by Sanger sequencing, 23% with a custom panel, 24% with a commercial panel and 32% with whole exome sequencing. A genetic study using NGS allows the study of a larger number of genes associated with RTT-like symptoms simultaneously, providing genetic study of a wider group of patients as well as significantly reducing the response time and cost of the study