EXPERIMENTAL AND COMPUTATIONAL STUDY OF BINDING INTERACTION OF ALKOXY DERIVATIVES OF N-ARYLHYDROXAMIC ACIDS WITH DNA

Objective: Binding affinity towards DNA for small molecules is very important in the development of new therapeutic reagents. Interaction between N-Arylhydroxamic acid derivatives N-p-Tolyl-4-Ethoxybenzohydroxamic acid (p-TEBHA) and N-o- Tolyl-4-Ethoxybenzohydroxamic Acid (o-TEBHA), with calf thymus DNA (CT DNA) were studied by UV–visible absorption, fluorescence,  viscosity measurement and molecular docking. Methods: The absorption and emission spectra of DMSO solution of hydroxamic acid derivatives were studied for their binding activity with calf-thymus DNA by titration with increasing amount hydroxamic acids. Docking was performed by HEX software. Results: Fluorescent spectra showed that CT DNA quenches the emission spectra of p-TEBHA & o-TEBHA with binding constant 1.6 Ñ… 108 M-1 and 4.3 Ñ… 103 M-1 respectively. Competitive study with ethidium bromide (EB) indicates that p-TEBHA can displace the DNA-bound EB suggesting strong competition with EB whereas o-TEBHA does not displace the DNA-bounded Ethidium bromide effectively. UV study of the interaction of the complexes with calf-thymus DNA has shown that the hydroxamic acid derivatives can bind with CT DNA. The docking studies were used to predict the mode of interaction of the drug with DNA. It was observed that as far as binding strength was concerned the computational results complemented the experimental results. Conclusion: Investigated hydroxamic acid derivatives are found to be strong DNA binders and seem to have promising drug like nature

Degradation of water quality due to heavy pollution in industrial area of Korba, Chhattisgarh

The  present  investigation were undertaken to study chemical & physical properties of Effluents  discharged from  korba  Industrial area. Industrial waste if discharged into surface water can give rise to significant deterioration in its quality. This paper presents groundwater quality of korba industrial area in korba City. Nine different locations were selected for the study and compared. The parameters studied were pH, total alkalinity, total hardness, turbidity, chloride, sulphate, fluoride, total dissolved solids and conductivity. From overall analysis, it was observed that there was a slight fluctuation in the physico-chemical parameters among the water samples studied. Comparison of the physico-chemical parameters of the water sample with WHO and ICMR limits showed that the Surface  water is highly contaminated and account for health hazards for human use

An important role of coal and its calorific value on the performance of thermal power station: A case study

An investigation was undertaken to study the Physical and Chemical properties of coal in Korba district. Due to the Presence of lot of Coal mines, number of coal based thermal power stations are established in Korba district. So study has been carried out for assessment of coal quality, whether it is suitable for thermal power stations, by collectying sample from Gevra Coal mines. This paper presents Grade of the coal available in Korba district. Three different Coal samples were collected from  different areas of Gevra Coal mines  and analyzed for Proximate, Ultimate and  Calorific value as per Standard methods. The useful heat values (UHV) of three coal samples are 2482K.Cal/Kg, 2917K.Cal/Kg, and 2786K.Cal/Kg. From overall analysis, and according to UHV of coal samples  we can conclude that  the grade of Gevra Coal is” F”and is very much  useful for Coal based thermal power stations

Investigation of some trace elements in Raipur Industrial area and its surrounding, Raipur District.

The paper deals with determination of eight trace metals namely. Copper, Iron, Mangnese, Zinc, Nickel,, Chromium  Lead and Mercury  in the ground water of different sites of Raipur Industrial area of Raipur district . All activities carried out on the ground surface have direct or indirect impact on the ground water whether associated with urban ,industrial or agricultural activities large scale concentrated source of pollutants, such as industrial discharges and sub surface injection of chemicals and hazardous are obvious source of ground water pollutants. This study was carryout in the month of during summer 2013. The samples were collected from seven different source of Raipur Industrial area of Raipur. The results obtained are compared with safe limits in ppm for heavy metals laid down by BIS, WHO, ICMR, APHA

Device-based password management system (bulwark)

Password manager systems currently available for the users come with serious privacy and vulnerability issues of their own. This paper gives an introduction to Bulwark, a novel device-based password management system, which stores the login credentials on a mobile device, and can enter the credentials on any target device through an encrypted cross-platform system, only after being authenticated by the user biometrics to ensure that only the right user can fill the saved passwords. This technology aims to eliminate the need to trust and rely on third-party cloud-based services or computers with storing and managing our passwords, and has also resolved major privacy and vulnerability issues with the current options available for users

N-Arylhydroxamic acids as a drug like molecule: A motif of binding mode with calf thymus DNA

215-221A drug-like molecule, which has a propensity of binding with DNA play a vital role in drug designing mechanism. In this paper, we tried to find out the DNA binding affinity of two derivatives of N-arylhydroxamic acids: (i) N-p-Chlorophenyl-2-methoxybenzohydroxamic Acid(Cl-2-MBHA) and (ii) N-p-Chlorophenyl-3-methoxybenzohydroxamic Acid (Cl-3-MBHA) with calf thymus DNA (ct-DNA) by applying techniques such as UV-visible spectroscopy, Fluorescence spectroscopy, and Viscometry measurements. The findings concluded with experimental techniques were verified with theoretical calculation using computer-based method, Molecular Docking. Absorption spectra revealed that both the hydroxamic acids derivatives bind to ct-DNA, among two, Cl-2-MBHA exhibits the higher value of binding affinity Kb (9.52 × 103±0.08 M-1). Fluorescence spectra showed that ct-DNA successfully quenches the emission spectra of N-arylhydroxamic acid. Ethidium bromide displacement method was used as a standard for analyzing the mode of binding. Both the hydroxamic acids were found to be groove binders. The Stern–Volmer Constant was found to be 2.05 × 10-2 ± 0.001 M-1 and 3.35 × 10-2 ± 0.002 M-1 for Cl-2-MBHA and Cl-3-MBHA respectively. Theoretical analysis molecular docking was done using Hex software for validating the experimental findings. Hence, it was observed that both experimental and computational method complimented the results and deduces groove binding as the mode of interaction