1,974 research outputs found
Negative frequency-dependent selection is intensified at higher population densities in protist populations
This is the final version of the article. Available from the Royal Society via the DOI in this record.Natural populations of free-living protists often exhibit high-levels of intraspecific diversity, yet this is puzzling as classic evolutionary theory predicts dominance by genotypes with high fitness, particularly in large populations where selection is efficient. Here, we test whether negative frequency-dependent selection (NFDS) plays a role in the maintenance of diversity in the marine flagellate Oxyrrhis marina using competition experiments between multiple pairs of strains. We observed strain-specific responses to frequency and density, but an overall signature of NFDS that was intensified at higher population densities. Because our strains were not selected a priori on the basis of particular traits expected to exhibit NFDS, these data represent a relatively unbiased estimate of the role for NFDS in maintaining diversity in protist populations. These findings could help to explain how bloom-forming plankton, which periodically achieve exceptionally high population densities, maintain substantial intraspecific diversity.E.J.A.M. was supported by a NERC research studentship (NE/H025472/2) as part of the UK Ocean Acidification Research Programme
The role of beak shape in octopodid taxonomy
Beak shapes in nine species of Southern Ocean octopodids were measured using seven size-standardized ratios. The results were analysed using principal component analysis and discriminant function analysis and showthat beak shape may be used as a taxonomic character to distinguish between genera, but not between species. Stepwise discriminant function analysis indicated that all seven ratios were required to maximize discrimination between beaks. A phenogram constructed from a matrix of Mahalanobis distances differed from a dendrogram produced from genetic data. This suggests that, although useful for discrimination, beak morphology is probably not suitable for constructing phylogenies
The transcriptome of the novel dinoflagellate Oxyrrhis marina (Alveolata: Dinophyceae): response to salinity examined by 454 sequencing
This is the final version of the article. Available from [BioMed Central via the DOI in this record.BACKGROUND: The heterotrophic dinoflagellate Oxyrrhis marina is increasingly studied in experimental, ecological and evolutionary contexts. Its basal phylogenetic position within the dinoflagellates make O. marina useful for understanding the origin of numerous unusual features of the dinoflagellate lineage; its broad distribution has lent O. marina to the study of protist biogeography; and nutritive flexibility and eurytopy have made it a common lab rat for the investigation of physiological responses of marine heterotrophic flagellates. Nevertheless, genome-scale resources for O. marina are scarce. Here we present a 454-based transcriptome survey for this organism. In addition, we assess sequence read abundance, as a proxy for gene expression, in response to salinity, an environmental factor potentially important in determining O. marina spatial distributions. RESULTS: Sequencing generated ~57 Mbp of data which assembled into 7, 398 contigs. Approximately 24% of contigs were nominally identified by BLAST. A further clustering of contigs (at ≥ 90% identity) revealed 164 transcript variant clusters, the largest of which (Phosphoribosylaminoimidazole-succinocarboxamide synthase) was composed of 28 variants displaying predominately synonymous variation. In a genomic context, a sample of 5 different genes were demonstrated to occur as tandem repeats, separated by short (~200-340 bp) inter-genic regions. For HSP90 several intergenic variants were detected suggesting a potentially complex genomic arrangement. In response to salinity, analysis of 454 read abundance highlighted 9 and 20 genes over or under expressed at 50 PSU, respectively. However, 454 read abundance and subsequent qPCR validation did not correlate well - suggesting that measures of gene expression via ad hoc analysis of sequence read abundance require careful interpretation. CONCLUSION: Here we indicate that tandem gene arrangements and the occurrence of multiple transcribed gene variants are common and indicate potentially complex genomic arrangements in O. marina. Comparison of the reported data set with existing O. marina and other dinoflagellates ESTs indicates little sequence overlap likely as a result of the relatively limited extent of genome scale sequence data currently available for the dinoflagellates. This is one of the first 454-based transcriptome surveys of an ancestral dinoflagellate taxon and will undoubtedly prove useful for future comparative studies aimed at reconstructing the origin of novel features of the dinoflagellates.This work was supported by a NERC grant (NE/F005237/1) awarded to PCW, DJSM, and CDL. We would like to thank Dr Margret Hughes of the Liverpool CGR for conducting 454 sequencing, and Dr Kevin Ashelford for invaluable scripting and bioinformatics support
Haemostasis in Open Carpal Tunnel Release: Tourniquet vs Local Anaesthetic and Adrenaline
Open carpal tunnel release is one of the commonest performed procedures in hand surgery. We performed a
prospective randomised control trial to compare the efficacy and patient satisfaction of the traditional arm tourniquet
versus infiltration of adrenaline and local anaesthetic solution to achieve haemostasis during the procedure. Using a
combination of objective and subjective measures we concluded that infiltration of local anaesthetic and adrenaline not
only provided adequate haemostasis but also provided a significantly more tolerable experience for the patient during the
procedure
Automatic Network Fingerprinting through Single-Node Motifs
Complex networks have been characterised by their specific connectivity
patterns (network motifs), but their building blocks can also be identified and
described by node-motifs---a combination of local network features. One
technique to identify single node-motifs has been presented by Costa et al. (L.
D. F. Costa, F. A. Rodrigues, C. C. Hilgetag, and M. Kaiser, Europhys. Lett.,
87, 1, 2009). Here, we first suggest improvements to the method including how
its parameters can be determined automatically. Such automatic routines make
high-throughput studies of many networks feasible. Second, the new routines are
validated in different network-series. Third, we provide an example of how the
method can be used to analyse network time-series. In conclusion, we provide a
robust method for systematically discovering and classifying characteristic
nodes of a network. In contrast to classical motif analysis, our approach can
identify individual components (here: nodes) that are specific to a network.
Such special nodes, as hubs before, might be found to play critical roles in
real-world networks.Comment: 16 pages (4 figures) plus supporting information 8 pages (5 figures
Mycobacterium tuberculosis osteomyelitis in a patient with human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS): a case report
The incidence of tuberculosis is increasing in the United States. Extra-pulmonary involvement is more common in patients with HIV/AIDS. The diagnosis of Tuberculosis osteomyelitis requires a high degree of suspicion for accurate and timely diagnosis
Individualization as driving force of clustering phenomena in humans
One of the most intriguing dynamics in biological systems is the emergence of
clustering, the self-organization into separated agglomerations of individuals.
Several theories have been developed to explain clustering in, for instance,
multi-cellular organisms, ant colonies, bee hives, flocks of birds, schools of
fish, and animal herds. A persistent puzzle, however, is clustering of opinions
in human populations. The puzzle is particularly pressing if opinions vary
continuously, such as the degree to which citizens are in favor of or against a
vaccination program. Existing opinion formation models suggest that
"monoculture" is unavoidable in the long run, unless subsets of the population
are perfectly separated from each other. Yet, social diversity is a robust
empirical phenomenon, although perfect separation is hardly possible in an
increasingly connected world. Considering randomness did not overcome the
theoretical shortcomings so far. Small perturbations of individual opinions
trigger social influence cascades that inevitably lead to monoculture, while
larger noise disrupts opinion clusters and results in rampant individualism
without any social structure. Our solution of the puzzle builds on recent
empirical research, combining the integrative tendencies of social influence
with the disintegrative effects of individualization. A key element of the new
computational model is an adaptive kind of noise. We conduct simulation
experiments to demonstrate that with this kind of noise, a third phase besides
individualism and monoculture becomes possible, characterized by the formation
of metastable clusters with diversity between and consensus within clusters.
When clusters are small, individualization tendencies are too weak to prohibit
a fusion of clusters. When clusters grow too large, however, individualization
increases in strength, which promotes their splitting.Comment: 12 pages, 4 figure
Rituximab for maintenance of remission in ANCA-associated vasculitis: expert consensus guidelines
Rituximab for maintenance of remission in ANCA-associated vasculitis: expert consensus guidelines—Executive summary
[This is the executive summary of Rituximab for maintenance of remission in ANCA-associated vasculitis: expert consensus guidelines: full guideline, doi: 10.1093/rheumatology/kez640
Haemostasis in Open Carpal Tunnel Release: Tourniquet vs Local Anaesthetic and Adrenaline
Open carpal tunnel release is one of the commonest performed procedures in hand surgery. We performed a
prospective randomised control trial to compare the efficacy and patient satisfaction of the traditional arm tourniquet
versus infiltration of adrenaline and local anaesthetic solution to achieve haemostasis during the procedure. Using a
combination of objective and subjective measures we concluded that infiltration of local anaesthetic and adrenaline not
only provided adequate haemostasis but also provided a significantly more tolerable experience for the patient during the
procedure
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