Comparison of digital image analysis methods for morphometric characterization of soil aggregates in thin sections

The purpose of this study was to investigate the applicability of semiautomatic segmentation methods for obtaining and evaluating morphometric parameters of soil aggregates in artificially prepared loose samples in soil thin sections. The object of the research is typical arable Chernozem. The aggregates were separated by wet sieving method from loose sample of upper 10 cm of the plowing horizon after erosion by a model shallow water flow on a large erosion tray. The aggregates, loosely scattered on the glass and fixed with polyester resin, were used to produce the thin sections. Images of the thin sections were taken under a polarizing microscope and then were processed using two methods compared: Adobe Photoshop + CTan and Thixomet Pro. Data on morphometric parameters of aggregates were obtained: the shape factor, the degree of roundness and the coefficient of aggregate surface roughness. The convergence of the results obtained using Photoshop + CTan by three researchers was evaluated by comparing samples using the Student's test and the Mann-Whitney test. The convergence of the averaged results obtained using Photoshop + CTan and the results obtained using Thixomet Pro was evaluated using the Mann - Whitney test. No significant differences were found between the parameters of the same aggregates obtained using a combination of Adobe Photoshop and CTan programs by different researchers. No significant differences were found between the parameters of the same aggregates obtained by the compared methods. So, one can conclude that the reliability of determining the morphometric parameters of soil aggregates using Thixomet Pro is comparable to the reliability of results when working with images of sectionsin CTan after binarization in Adobe Photoshop. The method of obtaining data on morphometric parameters of soil aggregates using Thixomet Pro completely eliminates the possibility of subjective error, shows a high degree of automation, reproducibility and reliability of the results obtained, and is faster

Purinergic modulation of microglial cell activation

Microglial cells are resident macrophages in the brain and their activation is an important part of the brain immune response and the pathology of the major CNS diseases. Microglial activation is triggered by pathological signals and is characterized by morphological changes, proliferation, phagocytosis and the secretion of various cytokines and inflammatory mediators, which could be both destructive and protective for the nervous tissue. Purines are one of the most important mediators which regulate different aspects of microglial function. They could be released to the extracellular space from neurons, astrocytes and from the microglia itself, upon physiological neuronal activity and in response to pathological stimuli and cellular damage. Microglial activation is regulated by various subtypes of nucleotide (P2X, P2Y) and adenosine (A1, A2A and A3) receptors, which control ionic conductances, membrane potential, gene transcription, the production of inflammatory mediators and cell survival. Among them, the role of P2X7 receptors is especially well delineated, but P2X4, various P2Y, A1, A2A and A3 receptors also powerfully participate in the microglial response. The pathological role of microglial purine receptors has also been demonstrated in disease models; e.g., in ischemia, sclerosis multiplex and neuropathic pain. Due to their upregulation and selective activation under pathological conditions, they provide new avenues in the treatment of neurodegenerative and neuroinflammatory illnesses

Impact of Treadmill Running and Sex on Hippocampal Neurogenesis in the Mouse Model of Amyotrophic Lateral Sclerosis

Hippocampal neurogenesis in the subgranular zone (SGZ) of dentate gyrus (DG) occurs throughout life and is regulated by pathological and physiological processes. The role of oxidative stress in hippocampal neurogenesis and its response to exercise or neurodegenerative diseases remains controversial. The present study was designed to investigate the impact of oxidative stress, treadmill exercise and sex on hippocampal neurogenesis in a murine model of heightened oxidative stress (G93A mice). G93A and wild type (WT) mice were randomized to a treadmill running (EX) or a sedentary (SED) group for 1 or 4 wk. Immunohistochemistry was used to detect bromodeoxyuridine (BrdU) labeled proliferating cells, surviving cells, and their phenotype, as well as for determination of oxidative stress (3-NT; 8-OHdG). BDNF and IGF1 mRNA expression was assessed by in situ hybridization. Results showed that: (1) G93A-SED mice had greater hippocampal neurogenesis, BDNF mRNA, and 3-NT, as compared to WT-SED mice. (2) Treadmill running promoted hippocampal neurogenesis and BDNF mRNA content and lowered DNA oxidative damage (8-OHdG) in WT mice. (3) Male G93A mice showed significantly higher cell proliferation but a lower level of survival vs. female G93A mice. We conclude that G93A mice show higher hippocampal neurogenesis, in association with higher BDNF expression, yet running did not further enhance these phenomena in G93A mice, probably due to a ‘ceiling effect’ of an already heightened basal levels of hippocampal neurogenesis and BDNF expression

Micromorphological evaluation of features of dry-land farming soils whithin kastanozems (chestnut) - solonetz soil complex in the Stavropol region

The polygenetic nature and polymorphism of the soils of kastanozems-solonetz complexes determine the difficulty of their field diagnostics and further genetic interpretations. In the current study, micromorphological features are used as additional diagnostic characteristics, specifying genetic interpretations for the soils of the kastanozems (chesynut)-solonetz complex (chestnut solonetz, chestnut solonetzic soil, chestnut soil, chestnut-like meadow soil) used in agriculture (dryland farming) within the Apanasenkovsky District of Stavropol region. An attempt has been made to correlate the results of micromorphological research, which was performed to evaluate the role of micromorphologycal investigation in clarification of macromorphological description, with data obtained through physical and chemical analyses. First, this study reveals that the Kastanozem (Chestnut) soil have the best correlation between macromorphological description and data of micromorphological investigation. In meadow-chestnut, solonetzic-type and solonetz soils micromorphological investigation helps to realize a set of pedofeatures such as complex multilayered coatings with layers of different genesis, Red-Ox features, etc. Finally, micromorphological investigation allows better interpretation and understanding of physico-chemical data

Realising the Carbon Benefits of Sustainable Land Management Practices: Guidelines for Estimation of Soil Organic Carbon in the Context of Land Degradation Neutrality Planning and Monitoring

Land degradation is one of the threats to human and natural systems. Fortunately, over the past few decades awareness of this challenge has grown, and 122 countries have committed to setting land degradation neutrality (LDN) targets, of which 84 have officially validated their targets, and 51 have put their targets into legislation. In this concept, LDN is achieved if new degradation is balanced by reversal of degradation elsewhere in the same land type by restoration or rehabilitation. The primary instrument for achieving LDN is through the implementation of sustainable land management (SLM) practices. Because of its multifunctional roles and its sensitivity to land management soil organic carbon (SOC) was selected as one of three indicators for LDN. Compared with the other global LDN indicators, that is, land cover change and land productivity dynamics (LPD) (measured as net primary productivity), SOC is challenging to manage and monitor at large scales. Moreover, SOC density in soils can vary greatly, even on the scale of meters, and fluctuates over time, for example between seasons. Comparative evaluation of SOC change between different SLM options (e.g. for land planning), tracking SOC dynamics through time (i.e. SOC monitoring) and effectively mapping SOC changes at large scales (e.g. for verifying LDN achievement) requires the combination of rigorous soil sampling schemes and the use of software tools/biophysical models for SOC assessment. To provide practical guidance to support the deployment of SLM interventions to maintain or enhance SOC stocks, for LDN and for other objectives such as landbased climate change adaption and/or mitigation a series of decision trees was developed, based on the latest available knowledge. This report reviews and compares available tools and models for SOC estimation. It presents practical guidance for land managers and puts forward policy-oriented proposals. Guidance for land managers emphasizes the selection of SLM practices to maintain or enhance soil organic carbon and achieve LDN. It addresses the choice of SLM practices suited to the local socio-economic and biophysical context; methods for measurement and monitoring of SOC; and the use of tools/ models for SOC assessment to estimate SOC and map SOC, and how to choose an appropriate tool/model according to the purpose. Policy-oriented options include to (i) share the guidance for land managers at the appropriate level; (ii) monitor SOC change as an indicator of SLM intervention to support assessment of LDN achievement in 2030; (iii) apply gender-responsive actions addressing gender-based differences and promote gender equality and women’s empowerment; (iv) design a framework for LDN Planning and means to support it

Discovery of Unusual Cyanobacterial Tryptophan-Containing Anabaenopeptins by MS/MS-Based Molecular Networking

Heterocytous cyanobacteria are among the most prolific sources of bioactive secondary metabolites, including anabaenopeptins (APTs). A terrestrial filamentous Brasilonema sp. CT11 collected in Costa Rica bamboo forest as a black mat, was studied using a multidisciplinary approach: genome mining and HPLC-HRMS/MS coupled with bioinformatic analyses. Herein, we report the nearly complete genome consisting of 8.79 Mbp with a GC content of 42.4%. Moreover, we report on three novel tryptophan-containing APTs; anabaenopeptin 788 (1), anabaenopeptin 802 (2), and anabaenopeptin 816 (3). Furthermore, the structure of two homologues, i.e., anabaenopeptin 802 (2a) and anabaenopeptin 802 (2b), was determined by spectroscopic analysis (NMR and MS). Both compounds were shown to exert weak to moderate antiproliferative activity against HeLa cell lines. This study also provides the unique and diverse potential of biosynthetic gene clusters and an assessment of the predicted chemical space yet to be discovered from this genus