Comportement des dromadaires sur patures Sahelien et Subhumide au Mali : 2. Especes vegetales et quantites de fourrage ingerees

Trois expériences ont été conduites entre 2014 et 2015 sur 12 dromadaires mâles adultes à Niono et 12 à Sotuba pour identifier les espèces broutées et la quantité de matière sèche ingérée correspondante en zones sahélienne et subhumide. Les données ont été collectées par suivi quotidien de 4 dromadaires porteurs de sac de collecte de fèces choisis au hasard, conduits avec le reste du troupeau durant 12 jours. A Niono, 32 espèces végétales ont été broutées et 71 espèces à Sotuba. A Niono, les espèces les plus broutées ont été Acacia seyal (19 %), Acacia senegal 14,8%), Balanites aegyptiaca (12,6 %) pour les ligneux et Zornia glochidiata (12,6 %) et Schoenefeldia gracilis (6,1 %) pour les graminées. A Sotuba, ce sont Ziziphus mauritiana (14,9 %), Vitellaria paradoxum (10,5 %) et les graminées Cassia tora (7,4 %) et Hiptis suaveolens (6,2 %). Dans le menu figuraient les feuilles, les rameaux, les fleurs et les fruits des espèces végétales consommées. Le taux moyen de protéines de l’ingéré a été de 13,3 % à Niono contre 8,7 % à Sotuba. La digestibilité de la matière sèche a été de 47,9 % à Niono contre 41,2 % à Sotuba. La teneur en protéines digestibles a été de 66,8 ± 0,8 g/kg MS à Niono contre 62,0 ± 0,7 g/kg MS à Sotuba. La quantité de matière sèche ingérée a été de 5,6 ± 0,2 kg à Niono et 5,1 ± kg à Sotuba. La connaissance de ces informations permettra de mieux organiser la complémentation alimentaire des dromadaires afin d’optimiser  leurs productions en zones sahélienne et subhumide du Mali. Mots clés : dromadaires, ingestion pâturage, sahel, subhumide, Mali English Title: Dromedary behaviour on Sahelian and subhumid pasture of Mali: 2. Plant species and feed intake Three experiments were conducted on 30 adult male camels to identify species grazed and the amount of dry matter ingested in the Sahelian (Niono) and sub-humid (Sotuba), zones during the rainy, cold dry and hot dry, seasons. Data were collected by monitoring 4 camels carrying faeces collection bags conducted with the rest of the herd. In Niono, 32 plant species were grazed and 71 species in Sotuba. The menu included leaves, twigs, flowers and fruits of consumed plant species. The average protein level of ingest was 13.3% in Niono and 8.7% in Sotuba. The digestibility of the material was 47.9% in Niono and 41.2% in Sotuba. The digestible protein content was 66.8 ± 0.8 g / kg of dry matter (DM) at Niono and 62.0 ± 0.7 g / kg DM at Sotuba. The amount of dry matter ingested was 5.6 ± 0.2 kg at Niono and 5.1 ± kg at Sotuba. Knowledge of this information is a key element to better organize animal feed supplementation in order to optimize camel production in the sahelian and sub-humid zones of Mali. Keywords: camels, grazing ingestion, Sahel, subhumid, Mal

Comportement des dromadaires adultes sur paturages sahelien et subhumide en station au Mali: 1. Description des principales activites

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Uptake of plasmodium falciparum gametocytes during mosquito bloodmeal by direct and membrane feeding

Plasmodium falciparum remains one of the leading causes of child mortality, and nearly half of the world’s population is at risk of contracting malaria. While pathogenesis results from replication of asexual forms in human red blood cells, it is the sexually differentiated forms, gametocytes, which are responsible for the spread of the disease. For transmission to succeed, both mature male and female gametocytes must be taken up by a female Anopheles mosquito during its blood meal for subsequent differentiation into gametes and mating inside the mosquito gut. Observed circulating numbers of gametocytes in the human host are often surprisingly low. A pre-fertilization behavior, such as skin sequestration, has been hypothesized to explain the efficiency of human-to-mosquito transmission but has not been sufficiently tested due to a lack of appropriate tools. In this study, we describe the optimization of a qPCR tool that enables the relative quantification of gametocytes within very small input samples. Such a tool allows for the quantification of gametocytes in different compartments of the host and the vector that could potentially unravel mechanisms that enable highly efficient malaria transmission. We demonstrate the use of our gametocyte quantification method in mosquito blood meals from both direct skin feeding on Plasmodium gametocyte carriers and standard membrane feeding assay. Relative gametocyte abundance was not different between mosquitoes fed through a membrane or directly on the skin suggesting that there is no systematic enrichment of gametocytes picked up in the skin

Blastic plasmacytoid dendritic cell leukemia in a black Malian

Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare, clinically aggressive hematologic malignancy, that most commonly manifests as cutaneous lesions. A 19-year-old Malian female was admitted to the Unit of Medicine of Hopital du Mali with anemia, fever, weakness, and weight loss. On physical examination she was wasted, pale, febrile (37.4°C), and had inguinal and axillary lymphadenopathies. The complete blood count found pancytopenia with Hemoglobin level of 4.8 g/dL, Leucocytes count of 1900/μL (neutrophil: 300/μL), and platelets count of 56 000/μL. The ultrasonographic examination found hepatomegaly and splenomegaly. The bone marrow biopsy and flow cytometer analysis were in keeping with a diagnosis of BPDCN. The patient, unfortunately, was lost four months later after her hospital admission due to late diagnosis by septicemia. The early diagnosis and availability of specific drugs for acute leukemia could improve the clinical outcome of patients with BPDCN in Mali.Keywords: Acute Leukemia, black african, dendritic cell, Mal

Protection of Malian children from clinical malaria is associated with recognition of multiple antigens

Contains fulltext : 153754.pdf (publisher's version ) (Open Access)BACKGROUND: Naturally acquired immunity to clinical malaria is thought to be mainly antibody-mediated, but reports on antigen targets are contradictory. Recognition of multiple antigens may be crucial for protection. In this study, the magnitude of antibody responses and their temporal stability was assessed for a panel of malaria antigens in relation to protection against clinical Plasmodium falciparum malaria. METHODS: Malian children aged two to 14 years were enrolled in a longitudinal study and followed up by passive and active case detection for seven months. Plasma was collected at enrolment and at the beginning, in the middle and after the end of the transmission season. Antibody titres to the P. falciparum-antigens apical membrane protein (AMA)-1, merozoite surface protein (MSP)-1(1)(9), MSP-3, glutamine-rich protein (GLURP-R0) and circumsporozoite antigen (CSP) were assessed by enzyme-linked immunosorbent assay (ELISA) for 99 children with plasma available at all time points. Parasite carriage was determined by microscopy and nested PCR. RESULTS: Antibody titres to all antigens, except MSP-1(1)(9), and the number of antigens recognized increased with age. After malaria exposure, antibody titres increased in children that had low titres at baseline, but decreased in those with high baseline responses. No significant differences were found between antibody titers for individual antigens between children remaining symptomatic or asymptomatic after exposure, after adjustment for age. Instead, children remaining asymptomatic following parasite exposure had a broader repertoire of antigen recognition. CONCLUSIONS: The present study provides immune-epidemiological evidence from a limited cohort of Malian children that strong recognition of multiple antigens, rather than antibody titres for individual antigens, is associated with protection from clinical malaria

Complications macro-angiopathiques du diabete a l’hopital du Mali de Bamako

Introduction-objectif: Le diabète est un facteur de risque cardiovasculaire (FRCV) majeur, responsable de complications cardiovasculaires dont la  prise en charge est complexe. L’objectif de l’étude est d’évaluer les  complications macro-angiopathiques du diabète.Patients et Méthodes: Etude transversale, descriptive et analytique de 18 mois, concernant les patients diabétiques (type 1 avec 5 ans d’évolution et type 2).Résultats: Nous avons colligé 275 patients diabétiques. L’âge moyen de nos patients était de 59 ans. Le sex ratio était de 0,82. Le diabète était de type 2 chez 93,09% des patients. La durée moyenne d’évolution du diabète était de 6 ans. L’HbA1c était supérieure à 7% chez 51,64% des patients. 77,46% avaient au minimum 2 FRCV associés au diabète. La dyslipidémie était le FRCV le plus fréquent associé au diabète (51,27%). Quatre-vingt-trois (83) patients sur 275 avaient au moins une  macroangiopathie (fréquence hospitalière de 30,18%). L’artériopathie oblitérante des membres inférieurs (AOMI) était présente chez 49 patients, l’accident vasculaire cérébral (AVC) chez 28 patients et la coronaropathie chez 17 patients. Nous avons noté un lien statistiquement significatif entre durée d’évolution du diabète, et deux complications macro-angiopathiques (AOMI p:0,001 et AVC p:0,05). Le mauvais équilibre glycémique et le nombre de FRCV étaient corrélés aux complications macroangiopathiques (AOMI, AVC, coronaropathie) de façon significative avec p<0,05.Conclusion: Le diabète est une maladie métabolique responsable de complications macro-angiopathiques. Sa prise en charge précoce ainsi qu’une correction des autres FRCV permettent d’éviter ou de ralentir ces complications.Mots clés : Diabète, Macro-angiopathies, Hôpital Du Mali.  Macrovascular complications of diabetes at the hôpital du Mali of Bamako Introduction-Aim: Diabete is a major risk cardiovascular factor (FRCV). It is a chronic disease responsible of cardiovascular complications with a complex management. The objective of the study is to evaluate the macrovascular complications of diabetes.Patients and methods: It is a cross-sectional, descriptive and analytical study of 18 months, concerning diabetic patients (type 1 with 5 years of evolution and type 2).Results: We collected 275 diabetics patients. Mean age of our patients was 59 years. Sex ratio was 0.82. Diabetes was type 2 in 93.09%. Mean duration of diabetes was 6 years. HBA1C was greater than 7% in 51.64%. 77.46% had at least 2 cardiovascular risk factors associated with diabetes. Dyslipidemia was the most common cardiovascular risk factors associated with diabetes (51.27%). 83 of 275 patients had at least one macrovascular complication (hospital frequency of 30.18%). Peripheral artery disease (PAD) was present in 49 patients, stroke in 28 patients, and coronary artery disease in 17 patients. We noted a statistically significant link between the duration of diabetes, and two macrovascular complications (PAD p: 0.001 and stroke p: 0.05). The poor glycemic balance and number of  cardiovascular risk factors were correlated with macrovascular complications (AOMI, stroke, coronary artery disease) significantly with p < 0.05.Conclusion: Diabetes is a metabolic disease responsible of macrovascular complications. Early care and correction of other cardiovascular risk factors can prevent or slow these complications.Keywords: Diabetes, Macrovascular complications, Hôpital Du Mal

Uptake of Plasmodium falciparum gametocytes during mosquito bloodmeal by direct and membrane feeding

Plasmodium falciparum remains one of the leading causes of child mortality, and nearly half of the world's population is at risk of contracting malaria. While pathogenesis results from replication of asexual forms in human red blood cells, it is the sexually differentiated forms, gametocytes, which are responsible for the spread of the disease. For transmission to succeed, both mature male and female gametocytes must be taken up by a female Anopheles mosquito during its blood meal for subsequent differentiation into gametes and mating inside the mosquito gut. Observed circulating numbers of gametocytes in the human host are often surprisingly low. A pre-fertilization behavior, such as skin sequestration, has been hypothesized to explain the efficiency of human-to-mosquito transmission but has not been sufficiently tested due to a lack of appropriate tools. In this study, we describe the optimization of a qPCR tool that enables the relative quantification of gametocytes within very small input samples. Such a tool allows for the quantification of gametocytes in different compartments of the host and the vector that could potentially unravel mechanisms that enable highly efficient malaria transmission. We demonstrate the use of our gametocyte quantification method in mosquito blood meals from both direct skin feeding on Plasmodium gametocyte carriers and standard membrane feeding assay. Relative gametocyte abundance was not different between mosquitoes fed through a membrane or directly on the skin suggesting that there is no systematic enrichment of gametocytes picked up in the skin

The Prevalence of Human <i>Plasmodium</i> Species during Peak Transmission Seasons from 2016 to 2021 in the Rural Commune of Ntjiba, Mali

Up-to-date knowledge of key epidemiological aspects of each Plasmodium species is necessary for making informed decisions on targeted interventions and control strategies to eliminate each of them. This study aims to describe the epidemiology of plasmodial species in Mali, where malaria is hyperendemic and seasonal. Data reports collected during high-transmission season over six consecutive years were analyzed to summarize malaria epidemiology. Malaria species and density were from blood smear microscopy. Data from 6870 symptomatic and 1740 asymptomatic participants were analyzed. The median age of participants was 12 years, and the sex ratio (male/female) was 0.81. Malaria prevalence from all Plasmodium species was 65.20% (95% CI: 60.10–69.89%) and 22.41% (CI: 16.60–28.79%) for passive and active screening, respectively. P. falciparum was the most prevalent species encountered in active and passive screening (59.33%, 19.31%). This prevalence was followed by P. malariae (1.50%, 1.15%) and P. ovale (0.32%, 0.06%). Regarding frequency, P. falciparum was more frequent in symptomatic individuals (96.77% vs. 93.24%, p = 0.014). In contrast, P. malariae was more frequent in asymptomatic individuals (5.64% vs. 2.45%, p P. ovale remained the least frequent species (less than 1%), and no P. vivax was detected. The most frequent coinfections were P. falciparum and P. malariae (0.56%). Children aged 5–9 presented the highest frequency of P. falciparum infections (41.91%). Non-falciparum species were primarily detected in adolescents (10–14 years) with frequencies above 50%. Only P. falciparum infections had parasitemias greater than 100,000 parasites per µL of blood. P. falciparum gametocytes were found with variable prevalence across age groups. Our data highlight that P. falciparum represented the first burden, but other non-falciparum species were also important. Increasing attention to P. malariae and P. ovale is essential if malaria elimination is to be achieved

The Prevalence of Human Plasmodium Species during Peak Transmission Seasons from 2016 to 2021 in the Rural Commune of Ntjiba, Mali

International audienceUp-to-date knowledge of key epidemiological aspects of each Plasmodium species is necessary for making informed decisions on targeted interventions and control strategies to eliminate each of them. This study aims to describe the epidemiology of plasmodial species in Mali, where malaria is hyperendemic and seasonal. Data reports collected during high-transmission season over six consecutive years were analyzed to summarize malaria epidemiology. Malaria species and density were from blood smear microscopy. Data from 6870 symptomatic and 1740 asymptomatic participants were analyzed. The median age of participants was 12 years, and the sex ratio (male/female) was 0.81. Malaria prevalence from all Plasmodium species was 65.20% (95% CI: 60.10–69.89%) and 22.41% (CI: 16.60–28.79%) for passive and active screening, respectively. P. falciparum was the most prevalent species encountered in active and passive screening (59.33%, 19.31%). This prevalence was followed by P. malariae (1.50%, 1.15%) and P. ovale (0.32%, 0.06%). Regarding frequency, P. falciparum was more frequent in symptomatic individuals (96.77% vs. 93.24%, p = 0.014). In contrast, P. malariae was more frequent in asymptomatic individuals (5.64% vs. 2.45%, p &lt; 0.001). P. ovale remained the least frequent species (less than 1%), and no P. vivax was detected. The most frequent coinfections were P. falciparum and P. malariae (0.56%). Children aged 5–9 presented the highest frequency of P. falciparum infections (41.91%). Non-falciparum species were primarily detected in adolescents (10–14 years) with frequencies above 50%. Only P. falciparum infections had parasitemias greater than 100,000 parasites per µL of blood. P. falciparum gametocytes were found with variable prevalence across age groups. Our data highlight that P. falciparum represented the first burden, but other non-falciparum species were also important. Increasing attention to P. malariae and P. ovale is essential if malaria elimination is to be achieved