Image Quality for Near-Eye Display Based on Holographic Waveguides

The paper analyzes the image quality in augmented reality display based on holographic waveguides. Brightness, brightness non-uniformity, image noise, etc., depend on the parameters of the waveguide substrate, the configuration, and the relief shape of diffraction optical elements. The optimal structure of holographic waveguides obtained by analog holography has been studied. The presented recommendations to achieve the best image quality are based on experimental results for different configurations of holographic waveguides

Data on morphology, large-scale chromatin configuration and the occurrence of proteins and rRNA in nucleolus-like bodies of fully-grown mouse oocytes in different fixatives

Here we provide data on accessibility of nucleolus-like bodies (NLBs) of fully-grown (GV) mouse oocytes to fluorescence in situ hybridization (FISH) probes and anti-nucleolar antibodies as well as on oocyte general morphology and large scale chromatin configuration, which relate to the research article “High-resolution microscopy of active ribosomal genes and key members of the rRNA processing machinery inside nucleolus-like bodies of fully-grown mouse oocytes” (Shishova et al., 2015 [1]). Experimental factors include: a cross-linking reagent formaldehyde and two denaturing fixatives, such as 70% ethanol and a mixture of absolute methanol and glacial acetic acid (3:1, v/v)

Human nucleolar protein SURF6/RRP14 participates in early steps of pre-rRNA processing.

The biogenesis of ribosomes requires tightly controlled transcription and processing of pre-rRNA which comprises ribosomal RNAs forming the core of large and small ribosomal subunits. Early steps of the pre-rRNA processing and assembly of the ribosomal subunits require a large set of proteins that perform folding and nucleolytic cleavage of pre-rRNAs in the nucleoli. Structure and functions of proteins involved in the pre-rRNA processing have been extensively studied in the budding yeast S. cerevisiae. Functional characterization of their human homologues is complicated by the complexity of mammalian ribosomes and increased number of protein factors involved in the ribosomal biogenesis. Homologues of human nucleolar protein SURF6 from yeast and mouse, Rrp14 and Surf6, respectively, had been shown to be involved in the early steps of pre-rRNA processing. Rrp14 works as RNA chaperone in complex with proteins Ssf1 and Rrp15. Human SURF6 knockdown and overexpression were used to clarify a role of SURF6 in the early steps of pre-rRNA processing in human cell lines HeLa and HTC116. By analyzing the abundance of the rRNA precursors in cells with decreased level or overexpression of SURF6, we demonstrated that human SURF6 is involved in the maturation of rRNAs from both small and large ribosomal subunits. Changes in the SURF6 level caused by knockdown or overexpression of the protein do not result in the death of HeLa cells in contrast to murine embryonic fibroblasts, but significantly alter the distribution of cells among the phases of the cell cycle. SURF6 knockdown in both p53 sufficient and p53 deficient HCT116 human cancer cells results in elongation of G0/G1 and shortening of G2/M phase. This surprising result suggests p53 independence of SURF6 effects on the cell cycle and possible multiple functions of SURF6. Our data point to the shift from pathway 1 to pathway 2 of the rRNA biogenesis caused by the SURF6 knockdown and its likely association with p53 pathway

Metabolic Alterations in Male-Sterile Potato as Compared to Male-Fertile

The common potato, Solanum tuberosum L., is the fourth most important agricultural crop worldwide. Until recently, vegetative propagation by tubers has been the main method of potato cultivation. A shift of interest to sexual potato reproduction by true botanical seeds is due to the appearance of a new hybrid seed breeding strategy whose successful application for many crop species has been supported by male sterility. This investigation was focused on the study of differences in the metabolite profiles of anthers at the mature pollen stage from male-fertile and male-sterile genotypes of S. tuberosum. Application of gas chromatography coupled with a mass spectrometry method allowed detection of metabolic profiles for 192 compounds. Further data analysis with several libraries fully identified 75 metabolites; a similar amount was defined up to the classes. Metabolic profiles in the anthers of fertile genotypes were significantly distinguished from male-sterile ones by the accumulation of carbohydrates, while the anthers of sterile genotypes contained a higher amount of amino acids. In comparison with male-fertile plants, male-sterile genotypes had undeveloped pollen grain characters; i.e., smaller grain size, a thicker exine, “permanent tetrads” that failed to disintegrate into microspores, and the absence of pollen apertures that might be due to a disorder in the metabolism of carbohydrates and fatty acids

Synbiotic yoghurt with walnut and cereal brittle added as a next‐generation bioactive compound: Development and characteristics

The article presents a technology developed for the production of synbiotic Yoghurt with new bioactive filler based on natural components. The Yoghurt has prebiotic and sorption properties. A higher consumer appeal of the product developed has been substantiated; its characteristics compared with the Yoghurt of traditional production technology have been presented. The brittle, containing peeled walnuts, as well as barley, wheat, rye, oatmeal and buckwheat flakes, sugar, and water, was used as a filler. Optimum time and temperature regimes of boiling caramel mixtures and brewing raw walnut–cereal mass in the brittle have been selected. The formulation developed enables increasing the nutritional and biological values of the finished product. The research studies of the finished product involved an analysis of organoleptic, physicochemical, and microbiological points. When performing the tasks, the approved regulatory and technical documentation (GOST) was applied. Each measurement was carried out in triplicate. The physicochemical characteristics of the samples developed were compared with the requirements for the quality of fermented milk products. The nutritional and biological values were calculated. The increase in consumer properties, and nutritional and biological values of the finished dairy product was scientifically substantiated and experimentally confirmed. HIGHLIGHTS A higher consumer appeal of the product developed has been substantiated; its characteristics compared with the Yoghurt of traditional production technology have been presented

The levels of peroxisomal catalase protein and activity modulate the onset of cell death in tobacco BY-2 cells via reactive oxygen species levels and autophagy

In plant cells, peroxisomes participate in the metabolism of reactive oxygen species (ROS). One of the major regulators of cellular ROS levels – catalase (CAT) – occurs exclusively in peroxisomes. CAT activity is required for immunity-triggered autophagic programmed cell death (PCD). Autophagy has been recently demonstrated to represent a route for degradation of peroxisomes in plant cells. In the present study, the dynamics of the cellular peroxisome pool in tobacco BY-2 cell suspension cultures were used to analyse the effects of inhibition of basal autophagy with special attention to CAT activity. Numbers of peroxisomes per cell, levels of CAT protein and activity, cell viability, ROS levels and expression levels of genes encoding components of antioxidant system were analysed upon application of 3-methyladenine (3-MA), an inhibitor of autophagy, and/or aminotriazole (AT), an inhibitor of CAT. When applied separately, 3-MA and AT led to an increase in cell death, but this effect was attenuated by their simultaneous application. The obtained data suggest that both the levels of CAT protein in peroxisomes as well as CAT activity modulate the onset of cell death in tobacco BY-2 cells via ROS levels and autophagy

Immunogenicity and Safety of Inactivated Sabin-Strain Polio Vaccine “PoliovacSin”: Clinical Trials Phase I and II

Global polio eradication requires both safe and effective vaccines, and safe production processes. Sabin oral poliomyelitis vaccine (OPV) strains can evolve to virulent viruses and result in poliomyelitis outbreaks, and conventional inactivated poliomyelitis vaccine (Salk-IPV) production includes accumulation of large stocks of neurovirulent wild polioviruses. Therefore, IPV based on attenuated OPV strains seems a viable option. To increase the global supply of affordable inactivated vaccine in the still not-polio free world we developed an IPV made from the Sabin strains–PoliovacSin. Clinical trials included participants 18–60 years of age. A phase I single-center, randomized, double-blind placebo-controlled clinical trial included 60 participants, who received one dose of PoliovacSin or Placebo. A phase II multicenter, randomized, double-blind, comparative clinical trial included 200 participants, who received one dose of PoliovacSin or Imovax Polio. All vaccinations were well tolerated, and PoliovacSin had a comparable safety profile to the Placebo or the reference Imovax Polio preparations. A significant increase in neutralizing antibody levels to polioviruses types 1–3 (Sabin and wild) was observed in PoliovacSin and Imovax Polio vaccinated groups. Therefore, clinical trials confirmed good tolerability, low reactogenicity, and high safety profile of the PoliovacSin and its pronounced immunogenic properties. The preparation was approved for clinical trials involving infants

Concept riqualificazione aree facoltà architettura politecnico di Milano. supervisor: santiago quesada

(From curators) The MIAW.2/forests program ///Day 1 Salutations Alessandro Balducci, Vice Rector of the Politecnico di Milano - Gianni Scudo, Vice Dean of the School ʻArchitettura e Societàʼ - Ilaria Valente, President School ʻArchitettura e Societàʼ Master of Sciences in Architecture Course - Emilio Genovesi, Councillorship of Culture, Expo, Fashion and Design, Comune di Milano - Richard Haag, Professor Emeritus University of Washington, Seattle // Opening Lecture ʻStick-Chartsʼ by Christine Dalnoky, Atelier Christine Dalnoky (France) // Workshops opening ///Day 2 MIAW Open Lectures Morning Sessions Françoise Akinosho / Maja Leonelli - MIAW Open Lectures Afternoon Sessions Anders Berensson + Ulf Mejergren (Visiondivison) / Drew Seskunas and Brice Delarue (Zirkumflex) ///Day 3 MIAW Open Lectures Morning Sessions Marialena Nikolopoulou / Marta Bottero and Chiara DʼAlpaos - MIAW Afternoon Open Lectures Sessions Giovanni Attili / Santiago Quesada ///Day 4 MIAW Open Lectures Morning Sessions Duilio Forte (AtelierFORTE) / Andre Dekker (Observatorium) ///Day 5 MIAW.2 Final and Farewell Party at the Politecnico di Milano Leonardo Campus /// The 2011 MIAWers are:/// 1 Rachele Albini / Giada Albonico / Jacopo Biasio / Sara Caramaschi / Elisa Carraro / Desislava Dimitrova / Cristina Gatti / Elisa Gulino / Mariya Hasamova / Nina Mikhailova / Ottavia Molatore / Joao Molinar / Azadeh Moradiasr / Mohyedin Navabzadeh Navabi / Giuseppe Maria Palermo / Riccardo Somaini / Bogdan Stojanovic /// 2 Andra Elena Apostolescu / Valentina Armenti / Dana Baranes / Sofia Chicherina / Guadalupe Ciocoletto / Marina Franzi / Gulin Keskin / Somayeh Khoshdelmishamandani / Gabriella Milo / Filippo Minora / Ayca Ozbank / Priyank Dipakbhai Parmar / Elena Piazza / Nicolò Rota / Pietro Spazzini / Paolo Volpetti / Tamara Zarkovic / Maia Zheliazkova /// 3 Kalina Andreeva / Giacomo Ardesio / Giulia Cascella / Filippo Cima / Carolina Luísa De Sá E Brito / Alaleh Emami / Michele Gerli / Anna Chiara Leardini / Marina Meloni / Navid Moshfeghi / Alexandra Nita / Rohit Perla / Gabriele Tomassini /// 4 Alessia Albini / Laura Beltrami / Giuseppe Biscottini / Lorenzo Campini / Angela Charalambous / Michela Di Noia / Reza Kamyabi / Ivana Lilikj / Jacopo Marcolini / Behnam Meimandpour / Surajkumar Nandakumar / Sophia Theologou / Ofelia Vera / Marco Zanini / Alice Cristina Jola Zingales / Riccardo Zocche /// 5 Fabio Bonaventura / Mattia Bravin / Matteo Calati / Lia Crupi / Serena DʼAndrea / Maria Grubova / Ketaki Kadam / Maria Korobitsyna / Aly Mohamed Mohamed Ahmed El Sayed / Mattia Morandi / Haitham Nabil Mohamed Mousa / Ozge Noyan / Arlinda Sheqiri / Ceren Sipahioglu / Paula Stefan / Claudio Valcamonico /// 6 Alessandra D'Apolito / Chrysafina Geronta / Bernardo Gonzalez De Cossio Paez / Rajesh Gupta / Sona Keresztesová / Federico Montagna / Maria Nickolova / Parsa Pahlavan / Raluca-Mihaela Serdaru / Alexandra Shatskaya /// 7 Elena Alexandrova / Chrystalla Argyrou / Tijana Djordjevic / Gloria Ferrari / Priscilla Gazzellini / Deborah Gotti / Francesco Ernst Lupic / Giulia Marcosano / Lara Mondonico / Veronica Notaro / Savia Palate / Caterina Pilar Palumbo / Ilaria Pedrini / Andrea Perletti / Giulia Repetti / Aster Sittoni / Gulcin Tanil / Matteo Tressi / Erica Ubbiali / Ayman Wagdy Mohamed Ibrahim /// 8 Giada Bonatti / Matteo Chelazzi / Alberto DʼOspina / Serena Di Nocera / Katarzyna Dudycz / Nenad Duric / Filip Durickovic / Francesca Fabiana Fochi / Andrea Guaiti / Daniela Iallorenzi / Americo Iannazzone / Mickeal Milocco / Gabriele Motta / Andreea Onu / Patrizio Pelosi / Marta Petrov / Olga Shishova / Jue Wang /// 9 Constantina Agroti / Arezoo Akbarvand / Glykeria Anaxagorouglyka / Stefano Arrighi / Nicolò Bianchi / Giulia Borghi / Miruna-Luiza Caragheorgheopol / Daniele Cremaschi / Vedat Ali Dalokay / Matteo Gobbi / Seyedeh Golkoo Hooshmand Ziafi / Daniele Iori / Berk Kangoz / Katarzyna Krol / Georgios Kyriazis / Lorenza Manfredi / Abdulkadir Eren Ozturk / Amanda Parisi De Marchi / Isha Rathod / Negin Salamat / Marina Serratine Paul / Alessia Tosini / Maria Vassileva / Ege Yuksek /// 10 Stefan Andelkovic / Mirko Andolina / Pietro Bodria / Alessandro Danesi / Irene De Donatis / Claudia De Flumeri / Corinna Del Bianco / Joris Katkecivius / Claudia Mainardi / Federico Marcantognini / Jenni Mazzoni / Luca Menegaldo / Milena Meregalli / Emanuele Migliorosi / Sara Moriggia / Manuele Mossoni / Claudia Praderio / Filippo Tiozzo / Arianne Veloce / Francesca Zanotto MIAW.2/forestsThe 2nd Milano International Architectural Workshop of the Politecnico di Milanoʼs School of ʻArchitettura e Societàʼ programmes, playing with the metaphor of forests, aimed to generate 10 new visions to explain nowadays living and its next future in the spirit of green design, resilience, recycling, and ethical consciousness.MIAW.2 took place from September 26th to September 30th 2011 involving more than 100 students supervised by 15 international tutors working on 10 different workshop themes with the target to enrich the Politecnico di Milanoʼs Leonardo Campus environscape with light installations.This catalogue shows the MIAW.2/forests resul