Measurement and correlation of solubilities for trifluoromethylbenzoic acid isomers in supercritical carbon dioxide

金沢大学大学院自然科学研究科生産プロセスThe solubilities of 2-, 3- and 4-trifluoromethylbenzoic acids in supercritical carbon dioxide were measured at 308.2, 313.2 and 323.2 K by a flow-type apparatus. The pressure range of the measurement was from 9 to 23 MPa. The enhancements of solubility by fluorination were markedly observed for 2- and 3-trifluoromethylbenzoic acids. The solubilities of 4-trifluoromethylbenzoic acids are almost the same as those of 4-methylbenzoic acid. The experimental data were correlated by the Peng-Robinson equation of state. The correlated results show good agreement with the experimental data by adjusting binary interaction parameters. The results can represent the tendency of solubility enhancement. The interaction parameter between carbon dioxide and 3-trifluoromethylbenzoic acid is smaller than that between carbon dioxide and 2-trifluoromethylbenzoic acid or methylbenzoic acid isomers. While the estimated sublimation pressure for 4-trifluoromethylbenzoic acid is smaller than those for the other compounds, the solubilities of 4-trifluoromethylbenzoic acids are almost the same as those of 4-methylbenzoic acid. The interaction parameter between carbon dioxide and 4-trifluoromethylbenzoic acid show the negative values. This fact indicates that the interaction between carbon dioxide and 4-trifluoromethylbenzoic acid is stronger than those between carbon dioxide and the other compounds. © 2004 Elsevier B.V. All rights reserved

Genetic Encoding of 3-Iodo-l-Tyrosine in Escherichia coli for Single-Wavelength Anomalous Dispersion Phasing in Protein Crystallography

SummaryWe developed an Escherichia coli cell-based system to generate proteins containing 3-iodo-l-tyrosine at desired sites, and we used this system for structure determination by single-wavelength anomalous dispersion (SAD) phasing with the strong iodine signal. Tyrosyl-tRNA synthetase from Methanocaldococcus jannaschii was engineered to specifically recognize 3-iodo-l-tyrosine. The 1.7 Å crystal structure of the engineered variant, iodoTyrRS-mj, bound with 3-iodo-l-tyrosine revealed the structural basis underlying the strict specificity for this nonnatural substrate; the iodine moiety makes van der Waals contacts with 5 residues at the binding pocket. E. coli cells expressing iodoTyrRS-mj and the suppressor tRNA were used to incorporate 3-iodo-l-tyrosine site specifically into the ribosomal protein N-acetyltransferase from Thermus thermophilus. The crystal structure of this enzyme with iodotyrosine was determined at 1.8 and 2.2 Å resolutions by SAD phasing at CuKα and CrKα wavelengths, respectively. The native structure, determined by molecular replacement, revealed no significant structural distortion caused by iodotyrosine incorporation

A 6-Year Controlled Gastric Adenocarcinoma Metastasized to the Lung, Cervical Spine and Mandible in a Japanese Male: A Patient Report

Gastric adenocarcinoma metastasized to the lung, cervical vertebrae and mandible 6 years after gastrectomy in a 70-year-old man. When the man visited our clinic, he complained of pain in the left mandible with paralysis in the left lower lip and diffuse swelling with a fluctuation inside the left ramus of the mandible. Medium contrast computed tomography (CT) presented bone loss that looked like a wormhole at the left angle of the mandible. Magnetic resonance imaging (MRI) revealed abscess or osteomyelitis at the site. He showed no response despite treatment with antibiotics, and we suspected a neoplastic lesion. With a mandibular ramus specimen obtained by biopsy and examined histopathologically, adenocarcinoma of the salivary gland was strongly suspected. MRI presented a neoplastic lesion in his cervical vertebrae, and by biopsy he was diagnosed with adenocarcinoma. Thereafter, chest CT presented multiple pulmonary metastases. Considering the patient’s history of gastrectomy due to gastric adenocarcinoma, the stomach, cervical vertebrae or mandible were examined pathologically and immunohistochemically by biopsy: all specimens showed a moderately differentiated type of tubular adenocarcinoma, and the results for cytokeratin-related tumor markers were the same. We finally diagnosed him as having metastases from gastric adenocarcinoma to the lung, cervical vertebrae and mandible. Because the metastases had spread to multiple organs, the mandibular lesion was not treated, and terminal care in another facility was unavoidably selected. In making a differential diagnosis of multiple metastases, pathological and immunohistochemical examinations of metastatic lesions by biopsy were very useful based on the diagnostic imagings by CT and MRI

タブレットPCを用いた津波避難支援システムの提案

津波災害に対する防災・減災対策では，「自助」・「共助」・「公助」が不可欠である．「自助」としての津波防災対策としては，津波ハザードマップを入手し，事前に避難行動などを検討しておくことが考えられる．しかし，自助としての津波ハザードマップはあまり活用されておらず，共助として地域で実施される津波避難訓練にもいくつかの課題が指摘されている．そこで，本研究では，タブレットPC向けに開発されたオフライン型GISに着目し，津波ハザードマップやユーザの現在位置・移動軌跡を表示することが可能な津波避難支援システムを提案する．本提案システムのプロトタイプを用いて，北海道小樽市をフィールドとした基礎的な評価実験を行った．その結果，提案システムの基本機能に関して，おおむね良好な評価結果を得ることができた．マルチメディア，分散，協調とモパイル(DICOMO2013)シンポジウム北海道河東郡音更町平成25年7月10日（水）?12日（金

Serial pseudoprogression of metastatic malignant melanoma in a patient treated with nivolumab: a case report

Abstract Background Pseudoprogression refers to a specific pattern of response sometimes observed in malignant melanoma patients receiving treatment with immune-checkpoint inhibitors. Although cases with pseudoprogression documented once have been reported previously, there have been no case reports yet of pseudoprogression events documented twice during treatment. Case presentation A 55-year-old man underwent surgery for locally advanced esophageal malignant melanoma and received postoperative adjuvant interferon therapy. However, he presented with multiple liver and bone metastases at 6 months after the surgery, and was initiated on treatment with nivolumab 2 mg/kg every 3 weeks as the first-line treatment for recurrent disease. Follow-up computed tomography revealed that the liver metastases initially increased transiently in size, but eventually regressed. However, while the liver metastases continued to shrink, a new peritoneal nodule emerged, that also subsequently shrinked during the course of treatment with nivolumab. With only grade 1 pruritus, the patient continues to be on nivolumab treatment at 15 months after the induction therapy, with no progression observed after the second episode of pseudoprogression in the liver and peritoneal nodule. Conclusions We present the case of a patient with metastatic malignant melanoma who showed the unique response pattern of serial pseudoprogression during treatment with nivolumab. This case serves to highlight the fact that development of a new lesion may not always signify failure of disease control during treatment with nivolumab

Measurement and correlation of solubilities for trifluoromethylbenzoic acid isomers in supercritical carbon dioxide

The solubilities of 2-, 3- and 4-trifluoromethylbenzoic acids in supercritical carbon dioxide were measured at 308.2, 313.2 and 323.2 K by a flow-type apparatus. The pressure range of the measurement was from 9 to 23 MPa. The enhancements of solubility by fluorination were markedly observed for 2- and 3-trifluoromethylbenzoic acids. The solubilities of 4-trifluoromethylbenzoic acids are almost the same as those of 4-methylbenzoic acid. The experimental data were correlated by the Peng-Robinson equation of state. The correlated results show good agreement with the experimental data by adjusting binary interaction parameters. The results can represent the tendency of solubility enhancement. The interaction parameter between carbon dioxide and 3-trifluoromethylbenzoic acid is smaller than that between carbon dioxide and 2-trifluoromethylbenzoic acid or methylbenzoic acid isomers. While the estimated sublimation pressure for 4-trifluoromethylbenzoic acid is smaller than those for the other compounds, the solubilities of 4-trifluoromethylbenzoic acids are almost the same as those of 4-methylbenzoic acid. The interaction parameter between carbon dioxide and 4-trifluoromethylbenzoic acid show the negative values. This fact indicates that the interaction between carbon dioxide and 4-trifluoromethylbenzoic acid is stronger than those between carbon dioxide and the other compounds