Nuove frontiere della fotosintesi clorofilliana: la foglia artificiale

Per molti anni la scienza ha tentato di riprodurre il processo fotosintetico delle piante, che permette di utilizzare la luce del sole e l'anidride carbonica dell'atmosfera per produrre gli zuccheri necessari al loro metabolismo. Nella primavera del 2010 sui quotidiani compare la notizia rivoluzionaria della creazione di una foglia artificiale, capace di trasformare la luce in una fonte di energia utilizzabile dall'uomo. Su quali principi si basa questa scoperta? Quanto ha di analogo con i processi che avvengono nelle foglie? E quanto siamo vicini all'applicazione su vasta scala di questa innovazione tecnologica

Water Potential Gradients between Old and Developing Leaves in Lithops (Aizoaceae).

Lithops plants consist of a pair of opposite succulent leaves inserted on a short stem. The apical meristem produces a new pair of leaves within the old one every growing season, recycling water from the old leaves. Since there are no data on water relations between the two pairs of leaves, we measured leaf water potential at different stages of development with a pressure chamber. Osmotic potential of cell sap was measured with a cryoscopic osmometer and turgor pressure was calculated indirectly. Leaf water potentials were never very low even though plants were not irrigated. In old leaves water potential ranged between -0.5 and -0.28 MPa. In young leaves water potential increased with size from -1.05 to -0.5 MPa and was always lower than in the corresponding old leaves. The water potential gradient between old and new leaves was steeper in the early stages of development (0.6 MPa) and gradually decreased (0.15 MPa) when young leaves had almost completed their expansion. Our data show that in Lithops water movement from old to young leaves occurs according to a water potential gradient. The maintenance of this gradient may be ascribed to differences in turgor pressure, due to the more elastic and plastic walls of cells of young leaves. The possibility to perform a complete life cycle without external water supply is an extreme adaptation to the arid environment where Lithops grows

CHARACTERIZATION OF WATER RELATION TRAITS IN WILD SICILIAN POPULATIONS OF BRASSICA

The aims of this work are to Investigate the variability in water relation traits among wild Brassica relatives adapted to arid and saline environments and to Identify specific differences in physiological traits that may be used to improve cultivated relatives widely used in our diet, such as broccoli, cabbage and kale

Monitoring of alien aquatic plants in the inland waters of Sicily (Italy)

Updated and reliable data on the presence and distribution of alien aquatic plant species in Sicily are lacking, and there is a need to fill this gap for a proper and efficient management of freshwater ecosystems and biodiversity. This paper reviews the available knowledge about alien aquatic vascular plants in the inland waters of Sicily (Italy). The aim is to provide an updated checklist, as a first step in the study of the impact of those plants on the native species and ecosystems of this Mediterranean island. The paper focuses on the strictly aquatic species (hydrophytes), excluding emergent macrophytes. Four species were listed, all of them free-floating and with American origin. Most of them occur within protected areas, and their introduction in the island appears to be anthropogenic. A set of functional traits of the alien species, such as relative growth rate, leaf mass per area, nitrogen and carbon content, were screened. These traits are useful for assessing the species invasive potential compared to native ones

Is in vitro micrografting a possible valid alternative to traditional micropropagation in Cactaceae? Pelecyphora aselliformis as a case study

Several taxa of Cactaceae are endangered by overcollection for commercial purposes, and most of the family is included in the Convention on International Trade in Endangered Species of Fauna and Flora (CITES). Micropropagation may play a key role to keep the pressure off wild populations and contribute to ex situ conservation of endangered taxa. One of the limits of micropropagation is the species-specific requirement of plant regulators for each taxon and sometimes even for different genotypes. With the micrografting technique the rootstock directly provides the scion with the necessary hormonal requirements. In this paper we present data on in vitro grafting of Pelecyphora aselliformis Ehrenberg, an Appendix I CITES listed species critically endangered and sought after by the horticultural trade, on micropropagated Opuntia ficus-indica Miller. Apical and sub-apical scions of P. aselliformis were used to perform micrografting with a successful rate of 97 and 81 % respectively. Survival rate after ex vivo transfer was 85 %. We hypothesize that this method could be applied to other endangered, slow growing taxa of Cactaceae thus contributing to the conservation of this endangered family.Several taxa of Cactaceae are endangered by overcollection for commercial purposes, and most of the family is included in the Convention on International Trade in Endangered Species of Fauna and Flora (CITES). Micropropagation may play a key role to keep the pressure off wild populations and contribute to ex situ conservation of endangered taxa. One of the limits of micropropagation is the species-specific requirement of plant regulators for each taxon and sometimes even for different genotypes. With the micrografting technique the rootstock directly provides the scion with the necessary hormonal requirements. In this paper we present data on in vitro grafting of Pelecyphora aselliformis Ehrenberg, an Appendix I CITES listed species critically endangered and sought after by the horticultural trade, on micropropagated Opuntia ficus-indica Miller. Apical and sub-apical scions of P. aselliformis were used to perform micrografting with a successful rate of 97 and 81 % respectively. Survival rate after ex vivo transfer was 85 %. We hypothesize that this method could be applied to other endangered, slow growing taxa of Cactaceae thus contributing to the conservation of this endangered family

Recovery from water stress in laurel plants: influence of short term potassium fertilization

Plant xylem hydraulic conductance varies with changes in sap solute concentrations, particularly potassium, a phenomenon known as 'ionic effect'. In well-watered Laurus nobilis plants, short term potassium fertilization increased xylem sap potassium concentration, resulting in an increase in plant hydraulic conductance (Kplant), leaf-specific conductivity of the shoot (kshoot) and transpiration rate (Eplant). The ionic effect is enhanced in embolized stems, where it can compensate the cavitation-induced loss of hydraulic conductance. The aim of this work was to test if water-stressed potassium-starved laurel plants could recover earlier from stress when irrigated with a potassium solution instead of water. Two-year-old potted laurel seedlings, grown under potassium-starved conditions, were subjected to water stress by suspending irrigation until leaf conductance to water vapor (gL) dropped to less than 50% of its initial value and leaf water potential (YL) reached turgor loss point (YTLP). Plants were then irrigated either with water or 25 mM KCl and measurements were taken at 3, 6 or 24 hours after irrigation. No significant differences were found between the two groups of plants in terms of YL, gL, Eplant, Kplant or kshoot. Analysis of xylem sap potassium concentration showed there were no significant differences between treatments, and potassium levels were similar to those of potassium-starved well-watered plants. In conclusion, potassium uptake or release to xylem appeared to be impaired at least up to 24 hours after relief from water stress, so fertilization after the onset of stress did not result in any short term advantage for recovery from drought stress

Tamarix arborea var. arborea and Tamarix parviflora: Two species valued for their adaptability to stress conditions

The choice of stress resistant and highly adaptable species is a fundamental step for landscaping and ornamental purposes in arid and coastal environments such as those in the Mediterranean basin. The genus Tamarix L. includes about 90 species with a high endurance of adversity. We investigated the water relations and photosynthetic response of Tamarix arborea (Sieb. ex Ehrenb.) Bge. var. arborea and T. parviflora DC. growing in an urban environment. Both species showed no evidence of drought or salt stress in summer, and appeared to follow two strategies with T. arborea var. arborea investing in high carbon gain at the beginning of the summer, and then reducing photosynthetic activity at the end of the season, and T. parviflora showing lower but constant levels of photosynthetic activity throughout the vegetative season. For landscaping and ornamental purposes, we suggest T. arborea var. arborea when a fast-growing, high-cover species is necessary, and T. parviflora when less-invasive species are required

Leaf water relation traits in typical Sicilian varieties of Vitis vinifera L.

In Italy, grapevines are extensively cultivated, with Sicily representing one of the most significant wine regions. The high number of autochthonous grapevine varieties represents an important source of genetic diversity, and the many Sicilian varieties have anatomical and physiological traits that allow them to resist to different levels of drought stress. We investigated the water relation parameters of four cultivars of Vitis vinifera L. (Catarratto, Corinto, Nero d'Avola and Zibibbo) and characterized their leaf hydraulics. Measurements were conducted during summer on plants growing in the experimental field of the IBBR-CNR near Palermo. Daily patterns of leaf water potential (Yleaf) and stomatal conductance (gs) were measured in the field. Pressure-volume curves were constructed by the bench dehydration method to obtain leaf water potential at turgor loss point (Ytlp), osmotic potential at full rehydration (p0) and bulk modulus of elasticity (εmax). Leaf samples were collected to determine vein density using ImageJ. Major vein density was measured on digitally scanned leaves, while minor vein density was measured on photomicrographs of cleared and stained leaf portions

Investigation into the Use of Encorafenib to Develop Potential PROTACs Directed against BRAFV600E Protein

BRAF is a serine/threonine kinase frequently mutated in human cancers. BRAF(V600E) mutated protein is targeted through the use of kinase inhibitors which are approved for the treatment of melanoma; however, their long-term efficacy is hampered by resistance mechanisms. The PROTAC-induced degradation of BRAF(V600E) has been proposed as an alternative strategy to avoid the onset of resistance. In this study, we designed a series of compounds where the BRAF kinase inhibitor encorafenib was conjugated to pomalidomide through different linkers. The synthesized compounds maintained their ability to inhibit the kinase activity of mutated BRAF with IC(50) values in the 40–88 nM range. Selected compounds inhibited BRAF(V600E) signaling and cellular proliferation of A375 and Colo205 tumor cell lines. Compounds 10 and 11, the most active of the series, were not able to induce degradation of mutated BRAF. Docking and molecular dynamic studies, conducted in comparison with the efficient BRAF degrader P5B, suggest that a different orientation of the linker bearing the pomalidomide substructure, together with a decreased mobility of the solvent-exposed part of the conjugates, could explain this behavior

A first update on mapping the human genetic architecture of COVID-19

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