Pseudomonas aeruginosa can be detected in a polymicrobial competition model using impedance spectroscopy with a novel biosensor

Electrochemical Impedance Spectroscopy (EIS) is a powerful technique that can be used to elicit information about an electrode interface. In this article, we highlight six principal processes by which the presence of microorganisms can affect impedance and show how one of these - the production of electroactive metabolites - changes the impedance signature of culture media containing Pseudomonas aeruginosa. EIS, was used in conjunction with a low cost screen printed carbon sensor to detect the presence of P. aeruginosa when grown in isolation or as part of a polymicrobial infection with Staphylococcus aureus. By comparing the electrode to a starting measurement, we were able to identify an impedance signature characteristic of P. aeruginosa. Furthermore, we are able to show that one of the changes in the impedance signature is due to pyocyanin and associated phenazine compounds. The findings of this study indicate that it might be possible to develop a low cost sensor for the detection of P. aeruginosa in important point of care diagnostic applications. In particular, we suggest that a development of the device described here could be used in a polymicrobial clinical sample such as sputum from a CF patient to detect P. aeruginosa

Development of a diagnostic device to detect different pseudomonas aeruginosa phenotypes in medically relevant contexts

Pseudomonas aeruginosa, widely present in the environment, is well known for its ability to cause infection in immune compromised individuals. For example, P. aeruginosa is the leading cause of morbidity and mortality in patients with cystic fibrosis (CF). Here, we describe how Electrochemical Impedance Spectroscopy (EIS) can be used to detect the presence of four different strains of P. aeruginosa. Using a low cost, screen printed carbon electrode significant changes can be seen in impedance data in the presence of P. aeruginosa after 24 hours. Furthermore, through the use of a normalization technique whereby the phase angle of the impedance (a commonly used parameter) is divided by a starting measurement, it is possible to identify differences between a non-mucoid and mucoid strain of P. aeruginosa. Sensors based upon the techniques described here could be used in a number of healthcare scenarios, where there is a need for low cost, real time detection of P. aeruginosa, such as CF

Subtle traps in sedimentary basins and their importance to hydrocarbon exploration

This thesis uses high-quality 3D seismic data from the Broad Fourteens Basin (Southern North Sea), Espírito Santo Basin (SE Brazil), and Taranaki Basin (New Zealand) to characterise the evolution of geological structures related to differential compaction and subsidence; also known as subtle hydrocarbon traps. Each chapter tackles deformation over a different geological feature, spanning from salt-withdrawal basins, to submarine channel complexes and associated mass-transport deposits. These chapters subsequently discuss the impact the results have on the hydrocarbon industry. Included in these discussions are the importance of subtle traps on carbon capture and storage, local sealing potential, and reservoir distribution. The Broad Fourteens Basin dataset was used to investigate concentric faults associated with salt withdrawal from below Triassic units. Throw-depth and throw-distance plots helped to understand the growth histories of the concentric faults. It was shown that these faults formed as a result of the bending of strata due to differential subsidence during salt withdrawal. Slip tendency analyses assessed the likelihood for faults to reactivate and transmit fluids whenever pore fluid pressure is increased. This approach simulated a typical profile during carbon capture and storage. It was shown that concentric faults will reactivate if pore fluid pressures are increased above 30 MPa at the relevant sub-surface depths, leaking fluids (including stored CO2) past regional seal intervals in the basin. Data from the Espírito Santo Basin were first used to assess the timing and magnitude of differential compaction over a submarine channel complex. Thickness-relief models helped quantify both the variations in thickness in overburden strata. Smaller channels associated with downslope knickpoints were located within the channel complex. Differential compaction over channels produced four-way dip closures, as coarse-grained sediments were deposited at the knickpoint base. These provide adequate structural traps after early burial. The Espírito Santo Basin 3D survey was used in a third chapter to assess how differential compaction affected sediment distribution over a mass-transport deposit. As large remnant and rafted blocks entrained within the MTD were buried, differential compaction produced anticlines over them. This created a rugged seafloor and the topographic highs confined sediment moving downslope, allowing it to pond in discrete depocentres. Results from the data analysis chapters were compared with compaction-related structures documented in the published literature. A novel classification for subtle structural traps associated with differential compaction was produced, separating each feature into one of four types; Type A: folds over tectonic structures >2 km wide; Type B: folds over sedimentary packages, typically elongate, ~500 m to 5 km wide; Type C: folds over topographic features that are 20 m to 2 km wide; Type D: folds over sub-seismic/outcrop features no larger than 20 m. The results of the classification can be used as a first assessment when recognising a compaction-related fold and to rapidly assess its evolution and effectiveness as a subtle hydrocarbon trap

A mathematical modelling study of an athlete's sprint time when towing a weighted sled

This is the author's accepted manuscript. The final publication is available at Springer via http://dx.doi.org/10.1007/s12283-013-0114-2.This study used a mathematical model to examine the effects of the sled, the running surface, and the athlete on sprint time when towing a weighted sled. Simulations showed that ratio scaling is an appropriate method of normalising the weight of the sled for athletes of different body size. The relationship between sprint time and the weight of the sled was almost linear, as long as the sled was not excessively heavy. The athlete’s sprint time and rate of increase in sprint time were greater on running surfaces with a greater coefficient of friction, and on any given running surface an athlete with a greater power-to-weight ratio had a lower rate of increase in sprint time. The angle of the tow cord did not have a substantial effect on an athlete’s sprint time. This greater understanding should help coaches set the training intensity experienced by an athlete when performing a sled-towing exercise

Reducing Participation Costs via Incremental Verification for Ledger Systems

Ledger systems are applications run on peer-to-peer networks that provide strong integrity guarantees. However, these systems often have high participation costs. For a server to join this network, the bandwidth and computation costs grow linearly with the number of state transitions processed; for a client to interact with a ledger system, it must either maintain the entire ledger system state like a server or trust a server to correctly provide such information. In practice, these substantial costs centralize trust in the hands of the relatively few parties with the resources to maintain the entire ledger system state. The notion of *incrementally verifiable computation*, introduced by Valiant (TCC \u2708), has the potential to significantly reduce such participation costs. While prior works have studied incremental verification for basic payment systems, the study of incremental verification for a general class of ledger systems remains in its infancy. In this paper we initiate a systematic study of incremental verification for ledger systems, including its foundations, implementation, and empirical evaluation. We formulate a cryptographic primitive providing the functionality and security for this setting, and then demonstrate how it captures applications with privacy and user-defined computations. We build a system that enables incremental verification, for applications such as privacy-preserving payments, with universal (application-independent) setup. Finally, we show that incremental verification can reduce participation costs by orders of magnitude, for a bare-bones version of Bitcoin

Prospective comparison of long-term pain relief rates after first-time microvascular decompression and stereotactic radiosurgery for trigeminal neuralgia

OBJECTIVE Common surgical treatments for trigeminal neuralgia (TN) include microvascular decompression (MVD), stereotactic radiosurgery (SRS), and radiofrequency ablation (RFA). Although the efficacy of each procedure has been described, few studies have directly compared these treatment modalities on pain control for TN. Using a large prospective longitudinal database, the authors aimed to 1) directly compare long-term pain control rates for first-time surgical treatments for idiopathic TN, and 2) identify predictors of pain control. METHODS The authors reviewed a prospectively collected database for all patients who underwent treatment for TN between 1997 and 2014 at the University of California, San Francisco. Standardized collection of data on preoperative clinical characteristics, surgical procedure, and postoperative outcomes was performed. Data analyses were limited to those patients who received a first-time procedure for treatment of idiopathic TN with > 1 year of follow-up. RESULTS Of 764 surgical procedures performed at the University of California, San Francisco, for TN (364 SRS, 316 MVD, and 84 RFA), 340 patients underwent first-time treatment for idiopathic TN (164 MVD, 168 SRS, and 8 RFA) and had > 1 year of follow-up. The analysis was restricted to patients who underwent MVD or SRS. Patients who received MVD were younger than those who underwent SRS (median age 63 vs 72 years, respectively; p 5 years of follow-up (60 of 164 and 64 of 168 patients, respectively). Immediate or short-term (< 3 months) postoperative pain-free rates (Barrow Neurological Institute Pain Intensity score of I) were 96% for MVD and 75% for SRS. Percentages of patients with Barrow Neurological Institute Pain Intensity score of I at 1, 5, and 10 years after MVD were 83%, 61%, and 44%, and the corresponding percentages after SRS were 71%, 47%, and 27%, respectively. The median time to pain recurrence was 94 months (25th–75th quartiles: 57–131 months) for MVD and 53 months (25th–75th quartiles: 37–69 months) for SRS (p = 0.006). A subset of patients who had MVD also underwent partial sensory rhizotomy, usually in the setting of insignificant vascular compression. Compared with MVD alone, those who underwent MVD plus partial sensory rhizotomy had shorter pain-free intervals (median 45 months vs no median reached; p = 0.022). Multivariable regression demonstrated that shorter preoperative symptom duration (HR 1.005, 95% CI 1.001–1.008; p = 0.006) was associated with favorable outcome for MVD and that post-SRS sensory changes (HR 0.392, 95% CI 0.213–0.723; p = 0.003) were associated with favorable outcome for SRS. CONCLUSIONS In this longitudinal study, patients who received MVD had longer pain-free intervals compared with those who underwent SRS. For patients who received SRS, postoperative sensory change was predictive of favorable outcome. However, surgical decision making depends upon many factors. This information can help physicians counsel patients with idiopathic TN on treatment selection

Prescription Opioid Use among Pregnant Women Enrolled in Rhode Island Medicaid

Objective: Our objective was to identify the patterns of opioid use among pregnant women enrolled in RI Medicaid. Methods: This study used linked RI Medicaid and RI Birth Certificate data from 01/01/2006 to 12/31/2016. We examined temporal trends of prescription opioid dispensings and identified risk factors associated with opioids use during pregnancy. Results: Among 25,500 RI Medicaid enrolled pregnant women who delivered a live baby from 2008 to 2016, 1,914 (7.5%) received at least one prescription for an opioid medication during pregnancy, 810 (3.2%) were during the first trimester, 633 (2.5%) during the second trimester, and 866 (3.4%) during the third trimester. Of these, 213 (0.8%) women received 3 or more opioids during pregnancy. The prevalence of prescription opioids dispensed in pregnant women increased from 4.9% in 2008 to 9.6% in 2015 (β SD: 0.66 0.28, P=0.05). Conclusions: Prescription opioid use during pregnancy has increased among women enrolled in RI Medicaid