High-Throughput SNP Genotyping

Whole genome approaches using single nucleotide polymorphism (SNP) markers have the potential to transform complex disease genetics and expedite pharmacogenetics research. This has led to a requirement for high-throughput SNP genotyping platforms. Development of a successful high-throughput genotyping platform depends on coupling reliable assay chemistry with an appropriate detection system to maximise efficiency with respect to accuracy, speed and cost. Current technology platforms are able to deliver throughputs in excess of 100 000 genotypes per day, with an accuracy of >99%, at a cost of 20–30 cents per genotype. In order to meet the demands of the coming years, however, genotyping platforms need to deliver throughputs in the order of one million genotypes per day at a cost of only a few cents per genotype. In addition, DNA template requirements must be minimised such that hundreds of thousands of SNPs can be interrogated using a relatively small amount of genomic DNA. As such, it is predicted that the next generation of high-throughput genotyping platforms will exploit large-scale multiplex reactions and solid phase assay detection systems

PHOENIX: Public Health and Obesity in England – the New Infrastructure eXamined First interim report: the scoping review

The PHOENIX project aims to examine the impact of structural changes to the health and care system in England on the functioning of the public health system, and on the approaches taken to improving the public’s health. The scoping review has now been completed. During this phase we analysed: Department of Health policy documents (2010-2013), as well as responses to those documents from a range of stakeholders; data from 22 semi-structured interviews with key informants; and the oral and written evidence presented at the House of Commons Communities and Local Government Committee on the role of local authorities in health issues. We also gathered data from local authority (LA) and Health and Wellbeing Board (HWB) websites and other sources to start to develop a picture of how the new structures are developing, and to collate demographic and other data on local authorities. A number of important themes were identified and explored during this phase. In summary, some key points related to three themes - governance, relationships and new ways of working - were: The reforms have had a profound effect on leadership within the public health system. Whilst LAs are now the local leaders for public health, in a more fragmented system, leadership for public health appears to be more dispersed amongst a range of organisations and a range of people within the LA. At national level, the leadership role is complex and not yet developed (from a local perspective). Accountability mechanisms have changed dramatically within public health, and many people still seem to be unclear about them. Some performance management mechanisms have disappeared, and much accountability now appears to rely on transparency and the democratic accountability that this would (theoretically) enable. The extent to which ‘system leaders’ within PHE are able to influence local decisions and performance will depend on the strength of relationships principally between the LA and the local Public Health England centre. These relationships will take time to develop. Many people have faced new ways of working, in new settings, and with new relationships to build. Public health teams in LAs have faced the most profound of these changes, having gone from a position of ‘expert voice’ to a position where they must defend their opinions and activities in the context of competing demands and severely restricted resources. Public health staff may require new skills, and may need to seek new ‘allies’ to thrive in the new environment. HWBs could be crucial in bringing together a fragmented system and dispersed leadership. The next phase of data collection will begin in March with the initiation of case study work. National surveys will be conducted in June/July this year (2014), and at the same time the following year. In this work, we will further explore the following themes: relationships, governance, decision making, new ways of working, and opportunities and difficulties

Scottish Digital Network Panel: Final Report

The Panel was asked to make recommendations on how a Scottish Digital Network could be established and funded, taking as a starting point the conclusions of the Scottish Broadcasting Commission. The key requirement for the SDN is to "provide a secure and sustainable source of competition to the BBC for public service broadcasting within Scotland"

Chemistry of airborne particles from metallurgical processing

Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2003.Vita.Includes bibliographical references.Airborne particles fall into one of three size ranges. The nucleation range consists of nanoparticles created from vapor atom collisions. The decisive parameter for particle size and composition is the supercooling of the vapor. The accumulation range, which comprises particles less than 2 micrometers, consists of particles formed from the collision of smaller primary particles from the nucleation range. The composition of agglomerates and coalesced particles is the same as the bulk vapor composition. Coarse particles, the composition of which is determined by a liquid precursor, are greater than 1 micrometer and solidify from droplets whose sizes are controlled by surface, viscous, and inertial forces. The relationship between size and composition of airborne particles could be seen in welding fume, a typical metallurgical aerosol. This analysis was performed with a cascade impactor and energy dispersive spectrometry with both scanning electron microscopy (SEM-EDS) and scanning transmission electron microscopy (STEM-EDS). Other methods for properly characterizing particles were discussed. In the analysis, less than 10% of the mass of fume particles for various types of gas metal arc welding (GMAW) were coarse, while one-third of flux cored arc welding (FCAW) fume particles were coarse. Coarse particles had a composition closer to that of the welding electrode than did fine particles. Primary particles were not homogeneous. Particles larger than the mean free path of the carrier gas had the same composition as that of the vapor, but for particles 20 to 60 nanometers, smaller particles were more enriched in volatile metals than larger particles were. This was explained by the cooling path along the bubble point line of a binary phase diagram.(cont.) Particles were not necessarily homogenous internally. Because nanoparticles homogenize quickly, they may form in a metastable state, but will not remain in that state. In this analysis, the presence of multiple stable immiscible phases explains this internal heterogeneity. The knowledge contained herein is important for industries that depend on the properties of nanoparticles, and for manufacturing, where industrial hygiene is important because of respirable particle by-products, such as high-energy-density metallurgical processing.by Neil Travis Jenkins.Ph.D

Vulnerability to fuel and transport poverty

This briefing highlights the groups of people that academic research has identified as vulnerable to experiencing fuel poverty and transport poverty. Fuel and transport poverty are distributed across the UK, although the groups affected in each place can vary and the characteristics can be different depending on the location and make up of households

Modern languages and mentoring: Lessons from digital learning in Wales

This article considers the role that mentoring, and in particular online mentoring, can play in tackling the decline in modern language learning at GCSE level in Wales. • It evaluates Digi-Languages, a blended learning experience that pairs university student linguists with secondary school learners of languages to improve MFL uptake at GCSE. • This article examines the conception, design and early outcomes of DigiLanguages. • The article evaluates the experiential learning of the mentees (Year 9 learners) and explores the ethos underpinning resource development and the project’s key messaging around culture and languages. • The article provides recommendations for the expansion of Digi-Languages to support broader language policy objectives in Wales, including the Welsh Government’s policy of one million Welsh speakers by 2050. • The article concludes with suggestions for the extension of Digi-Languages to other regions of the UK and overseas and its potential as a model for stimulating inter-cultural conversations on the lifelong value of languages

A new approach for luminescence dating glaciofluvial deposits - High precision optical dating of cobbles

In recent years luminescence dating has increasingly been applied to date glaciofluvial sediments, but uncertainties about the degree of bleaching of the luminescence signal at deposition make dating of such sediments challenging. Here we test a new approach for luminescence dating of glaciofluvial sediments, based on the analysis of rock cores drilled from granite cobbles, and compare the luminescence ages generated against independent age control. Luminescence measurements from rock slices in cobble-sized clasts can be used to reconstruct the extent of bleaching, thereby giving greater confidence in the ages produced. This study illustrates that another important advantage of using cobbles is that at depths of 2 mm or more below the cobble surface >90% of the total dose rate arises from the cobble itself, making the dose rate insensitive to the water content of the sediment matrix. Ordinarily, uncertainties in estimating water content during burial are one of the largest sources of uncertainty in luminescence dating methods, and hence reducing the reliance upon the dose rate could be particularly advantageous for glacial deposits, where water contents can potentially be large and highly variable. Measurements of cobbles from Orrisdale Head, Isle of Man, demonstrate that the luminescence signal was completely bleached to depths of up to 12 mm into the cobble. Sampling of orientated cobbles from lithofacies diagnostic of bar-top environments was used to maximise the chances of exposure to sunlight. The upper-faces of these orientated cobble surfaces appear to be bleached to a greater depth than the lowermost faces. Data from 45 rock slices from these cobbles were tightly clustered, yielding a mean age of 20.7 ± 0.3 ka that is in agreement with independent age control. One of the well-bleached cobbles shows evidence of two discrete exposure events, potentially recording both the advance at 26.2 ± 0.8 ka, and retreat at 20.7 ± 0.3 ka, of the Irish Sea Ice Stream