Microbial Similarity between Students in a Common Dormitory Environment Reveals the Forensic Potential of Individual Microbial Signatures.

The microbiota of the built environment is an amalgamation of both human and environmental sources. While human sources have been examined within single-family households or in public environments, it is unclear what effect a large number of cohabitating people have on the microbial communities of their shared environment. We sampled the public and private spaces of a college dormitory, disentangling individual microbial signatures and their impact on the microbiota of common spaces. We compared multiple methods for marker gene sequence clustering and found that minimum entropy decomposition (MED) was best able to distinguish between the microbial signatures of different individuals and was able to uncover more discriminative taxa across all taxonomic groups. Further, weighted UniFrac- and random forest-based graph analyses uncovered two distinct spheres of hand- or shoe-associated samples. Using graph-based clustering, we identified spheres of interaction and found that connection between these clusters was enriched for hands, implicating them as a primary means of transmission. In contrast, shoe-associated samples were found to be freely interacting, with individual shoes more connected to each other than to the floors they interact with. Individual interactions were highly dynamic, with groups of samples originating from individuals clustering freely with samples from other individuals, while all floor and shoe samples consistently clustered together.IMPORTANCE Humans leave behind a microbial trail, regardless of intention. This may allow for the identification of individuals based on the "microbial signatures" they shed in built environments. In a shared living environment, these trails intersect, and through interaction with common surfaces may become homogenized, potentially confounding our ability to link individuals to their associated microbiota. We sought to understand the factors that influence the mixing of individual signatures and how best to process sequencing data to best tease apart these signatures

Changes in the basis of taxation: 1929-1936

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An evaluation of CGA-43089 to protect grain sorghum from alachlor and metolachlor injury

Field studies were conducted to determine: (1) if seed protectant CGA-43089 [α-(cyanomethoximino)-benzacetonitrile] will protect grain sorghum (Sorghum bicolor L. Moench.) from the phytotoxicity caused by alachlor [2-chloro-2\u27,6\u27-diethyl-N-(methoxymethyl)acetanilide] and metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-l-methylethyl)acetamide] applied at different rates, and (2) the susceptibility of two grain sorghum cultivars to alachlor and metolachlor applied with and without the protectant. The effects of CGA-43089 at two rates (0, and 1.25 g/kg of seed) and alachlor or metolachlor at four rates (0, 2.2, 3.4, and 4.5 kg/ha) on two grain sorghum cultivars (Funk G-251 and Funk G-623GBR) were studied in experiments conducted at the Main Experiment Station, Knoxville, the Ames Plantation Experiment Station, Grand Junction, and the Martin Experiment Station, Martin, during 1978. Parameters measured at each location were percent vigor reduction, plant population, panicle production, mature plant height, average grain sorghum panicle weight, and grain yield. Early herbicide injury, as noted by vigor and stand reduction, was later expressed as reduced yields where nontreated seed were used. In general, the vigor reduction was less at 60 days after planting than at 30 days after planting. With CGA-43089 seed treatment, plant population, panicle production, and grain yield tended to be greater. The effect of seed treatment or herbicide application on plant height and average grain sorghum panicle weight was somewhat inconsistent. Plants of cultivar Funk G-251 tended to be shorter than those of cultivar Funk G-623GBR across practically all treatments. In general, cultivar Funk G-251, although nonbird resistant, appears to be better suited for production in Tennessee than cultivar Funk G-623GBR due to earlier maturity

Public Attitudes and Gender Policy Regimes: Coherence and Stability in Hard Times

Drawing upon data from the European Social Survey on public attitudes and social welfare, this paper analyzes the extent to which attitudes toward gender equality in work and family life vary among 13 countries which represent different welfare regimes. The analysis also examines how these attitudes have changed with the onset of the economic recession in 2007. The findings suggest that public attitudes toward gender issues are largely consistent with welfare regimes, and most notably, reveal a clear direction of moving away from traditional views of gender, family and work issues in economic hard times

Social Security and The Incremental Privatization of Retirement Income

This study examines the process of incremental privatization of retirement income--a slow decline in the proportion of retirement income from Social Security relative to retirement income derived from employerprovided pensions (private and government) and IRA-type Accounts. The findings reveal that since the mid 1970s the elderly in the bottom 40% of the income distribution experienced a minimal increase in retirement income from pensions other than Social Security, while those in the upper ranges showed steady gains in income from private sources. This trend is accompanied by increasing inequality in the ratio of retirement income of those in the lower quintiles to those in the upper quintiles

Microbiome profile associated with malignant pleural effusion.

INTRODUCTION:There is ongoing research into the development of novel molecular markers that may complement fluid cytology malignant pleural effusion (MPE) diagnosis. In this exploratory pilot study, we hypothesized that there are distinct differences in the pleural fluid microbiome profile of malignant and non-malignant pleural diseases. METHOD:From a prospectively enrolled pleural fluid biorepository, samples of MPE were included. Non-MPE effusion were included as comparators. 16S rRNA gene V4 region amplicon sequencing was performed. Exact Sequence Variants (ESVs) were used for diversity analyses. The Shannon and Richness indices of alpha diversity and UniFrac beta diversity measures were tested for significance using permutational multivariate analysis of variance. Analyses of Composition of Microbiome was used to identify differentially abundant bacterial ESVs between the groups controlled for multiple hypothesis testing. RESULTS:38 patients with MPE and 9 with non-MPE were included. A subgroup of patients with metastatic adenocarcinoma histology were identified among MPE group (adenocarcinoma of lung origin (LA-MPE) = 11, breast origin (BA-MPE) = 11). MPE presented with significantly greater alpha diversity compared to non-MPE group. Within the MPE group, BA-MPE was more diverse compared to LA-MPE group. In multivariable analysis, ESVs belonging to family S24-7 and genera Allobaculum, Stenotrophomonas, and Epulopiscium were significantly enriched in the malignant group compared to the non-malignant group. CONCLUSION:Our results are the first to demonstrate a microbiome signature according to MPE and non-MPE. The role of microbiome in pleural effusion pathogenesis needs further exploration

Compassion on University Degree Programmes at a UK University: The Neuroscience of Effective Group work

This article is published under the Creative Commons Attribution (CC BY 4.0) licence. Anyone may reproduce, distribute, translate and create derivative works of this article (for both commercial and non-commercial purposes), subject to full attribution to the original publication and authors. The full terms of this licence may be seen at http://creativecommons.org/ licences/by/4.0/legalcodePurpose The purpose of this paper is to explore the neuroscience that underpins the psychology of compassion as a competency. We explain why this cognitive competency is now taught and assessed on modules of different degree subjects in a UK university. Design/methodology/approach The paper is divided into first, an exploration of recent psychology and neuroscience literature that illuminates the differences, and relationship, between empathy and compassion for safeness building in teams. Within that, the role of oxytocin in achieving social and intellectual rewards though the exercise of cognitive flexibility, working memory and impulsive inhibitory control (Zelazo, et al, 2016) is also identified. The literature findings are compared against relevant qualitative data from the above university’s, so far, nine years of mixed methods action research on compassion-focused pedagogy (CfP). Findings These are that the concept and practice of embedding compassion as a cognitive competency into assessed university group work is illuminated and rationalised by research findings in neuroscience. Research limitations/implications The limitations of the study are that, so far, fMRI research methods have not been used to investigate student subjects involved in the compassion-focused pedagogy now in use. Practical implications The paper has implications for theory, policy and practice in relation to managing the increasing amount of group work that accompanies widening participation in Higher Education. Originality/value A review of this kind specifically for student assessed group and its implications for student academic achievement and mental health has not, apparently, been publishedPeer reviewe

Impacts of indoor surface finishes on bacterial viability.

Microbes in indoor environments are constantly being exposed to antimicrobial surface finishes. Many are rendered non-viable after spending extended periods of time under low-moisture, low-nutrient surface conditions, regardless of whether those surfaces have been amended with antimicrobial chemicals. However, some microorganisms remain viable even after prolonged exposure to these hostile conditions. Work with specific model pathogens makes it difficult to draw general conclusions about how chemical and physical properties of surfaces affect microbes. Here, we explore the survival of a synthetic community of non-model microorganisms isolated from built environments following exposure to three chemically and physically distinct surface finishes. Our findings demonstrated the differences in bacterial survival associated with three chemically and physically distinct materials. Alkaline clay surfaces select for an alkaliphilic bacterium, Kocuria rosea, whereas acidic mold-resistant paint favors Bacillus timonensis, a Gram-negative spore-forming bacterium that also survives on antimicrobial surfaces after 24 hours of exposure. Additionally, antibiotic-resistant Pantoea allii did not exhibit prolonged retention on antimicrobial surfaces. Our controlled microcosm experiment integrates measurement of indoor chemistry and microbiology to elucidate the complex biochemical interactions that influence the indoor microbiome