Additional file 4: of Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

Alignement of the targeted Cry1Ab sequences from the T304–40 event sequenced from the PCR verification assay (1 and 2) with the reference sequence from the T304–40 event (reference) used for the design of the target-specific primers (surrounded by orange rectangles). (DOCX 506 kb

Additional file 1: of Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

The nucleotide variations, indicated by the red crosses, between the designed target-specific primers (Cry-F a, b and c) and the targeted sequences GM events possessing the CryAb/c elements (Bt11, MON87701, MON87751, MON531, Bt176, T304–40, Bt63, MON810, KeFeng-6, MON89034, MON15985 and 281–24-236 × 3006–210-23 events). (DOCX 15 kb

Additional file 5: of Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

Sequences of the sequenced amplicons from the processed food matrix (GeM MP17) coming from a GeMMA Scheme Proficiency Test. (DOCX 14 kb

Additional file 6: of Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

Sequences of oligonucleotides provided by the APAgeneTM GOLD Genome Walking Kit from BIO S&T. (DOCX 13 kb

Additional file 3: of Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

Sequences of the sequenced amplicons (see Tables 2 and 3). (DOCX 20 kb

Additional file 1: Figure S1. of Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin

Sequence of B. subtilis subsp. subtilis str. 168 genome sequence [Genbank:CP010052.1] corresponding to the region containing part of Contig00019 and Contig00022. There is a gap of 37 basepairs (uncoloured region in the figure) between Contig00019 (pink region in figure) and Contig00022 (yellow region in figure), when aligning the obtained contigs to B. subtilis subsp. subtilis str. 168 genome sequence [Genbank:CP010052.1]. PCR and sequence analysis were used to confirm that the genomic regions present on both contigs are indeed adjacent in the GM-Bacillus genome. Hereto, primers Scaf-19-F3-seq (positioned on Contig00019, indicated in green) and Scaf-22-R-seq (positioned on Contig00022, indicated in blue) were used to amplify the flanking regions of the junction between Contig00019 and Contig00022. Subsequently, the obtained PCR fragment was sequenced. (PDF 101 kb

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Sequences obtained from the GM maize MON863 event using the bidirectional p35S and tNOS DNA walking methods. The number of the corresponding amplicons observed in Fig. 2a is indicated in brackets. The maize genome and the transgenic cassette are designated respectively in small letter and capital letter. The nptII gene (underlined) is under the control of the p35S promoter (p35S; in italic) and the tNOS terminator (tNOS; in bold). The 4-AS1 promoter (p-4AS1; dashed underlined) is followed by the Wheat major chlorophyll a/b binding protein gene (wtCAB; wave underlined) and Rice Actin Intron (rAct; dotted underlined) to regulate the Cry3Bb1 gene (double underlined). (DOCX 16 kb

Additional file 1: Table S1. of The genetic structure of the Belgian population

SNP variant allele frequencies of the Belgian population, BelPHG-21 study, Belgium 2016. Variant allele frequencies (VAF) for all SNPs were calculated with reference to the human genome build hg19 on all samples or after exclusion of samples with a foreign origin (indicated by tab names). Columns indicate SNP ids, chromosome, position, reference allele, variant allele, VAF, and frequencies of homozygous reference (AA), heterozygous (AB), and homozygous variant (BB) alleles respectively. (XLSX 28534 kb

Satory, 4/2/25, concours d'endurance de véhicules pour l'armée, chenille Citroën 10 HP : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 98415Appartient à l’ensemble documentaire : Pho20RolImage de press