Additional file 1: Figure S1. of Mediator subunit Med12 contributes to the maintenance of neural stem cell identity

Unbiased genome-wide expression profiling analysis validation (A, C, D) RNA from mNS-5 NSCs infected with lentiviruses expressing non-specific (NS) control or Med12-specific shRNAs was harvested and subjected to RT-qPCR analysis. mRNA levels for each gene were normalized to β-actin mRNA and expressed relative to their corresponding mRNA levels in NS control shRNA-expressing cells. In (C, D) relative mRNA levels for 29 randomly selected genes from the original microarray-derived list were determined using the same RNA samples subjected to microarray analysis (C) as well as RNA from an independent set of knockdown experiments (D). Data represent the mean +/− SEM of at least three independent experiments performed in triplicate. Asterisks denote statistically significant differences relative to NS control shRNA (Student’s t-test, *p < 0.1, **p < 0.05, ***p < 0.01). (B) Nuclear extracts from mNS-5 NSCs infected with lentiviruses expressing non-specific (NS) control or Med12-specific shRNAs were harvested and resolved by SDS-10 % PAGE prior to processing by immunoblot using antibodies specific for Med12, Cdk8, the p89 subunit of transcription factor IIH (p89), and the β subunit of transcription factor IIE (TfIIeβ). Both p89 and TfIIeβ served as an internal loading controls. (TIF 16612 kb

Additional file 2: Table S1. of Mediator subunit Med12 contributes to the maintenance of neural stem cell identity

Differentially regulated genes by Med12 KD in mNS-5 NSCs. (DOCX 100 kb

Additional file 3: Figure S2. of Mediator subunit Med12 contributes to the maintenance of neural stem cell identity

Validation of Med12 and Cdk8 depletion in representative knockdown experiment. Nuclear extracts from mNS-5 NSCs infected with lentiviruses expressing non-specific (NS) control or Med12-specific shRNAs were harvested and resolved by SDS-10 % PAGE prior to processing by immunoblot using antibodies specific for Med12, Cdk8, the p89 subunit of transcription factor IIH (p89), the latter of which served as an internal loading control. (TIF 917 kb

Effects of radiofrequency field exposure on glutamate-induced oxidative stress in mouse hippocampal HT22 cells

<p><b>Purpose:</b> To define the impact of radiofrequency (RF) under <i>in vitro</i> experimental Alzheimer’s disease conditions, we investigated the effect of RF radiation on glutamate-induced oxidative stress in mouse hippocampal neuronal HT22 cells.</p> <p><b>Materials and methods:</b> Cell survival rate was measured by MTT and trypan blue exclusion assays. Cell cycle distribution, cell death, and ROS production were analyzed using flow cytometry. Expression of proteins was analyzed by Western blot.</p> <p><b>Results:</b> RF exposure alone had a marginal impact on cell proliferation; however, it significantly enhanced glutamate-induced cytotoxicity in HT22 cells. Glutamate augmented the subG1 fraction of cell cycle, annexin/propidium iodide positive cell population, and expression of cleaved poly (ADP ribose) polymerase, which were further increased by RF exposure. Glutamate induced reactive oxygen species (ROS) generation and RF exposure further upregulated it. N-acetylcysteine (NAC) treatment completely abrogated glutamate- and RF-induced ROS production followed by cell death and restored cell proliferation in HT22 cells. Finally, glutamate phosphorylated c-Jun N-terminal kinase (JNK) and RF increased this event further. Treatment with NAC and inhibitor of JNK decreased JNK phosphorylation and restored cell proliferation, respectively.</p> <p><b>Conclusions:</b> Our results demonstrate that RF exposure enhanced glutamate-induced cytotoxicity by further increase of ROS production in HT22 cells.</p

Media 4: Generation speed and reconstructed image quality enhancement of a long-depth object using double wavefront recording planes and a GPU

Originally published in Applied Optics on 01 August 2014 (ao-53-22-4817

Blending of political and media power: The case of Radek John and his programme Bez cenzury

This thesis considers the role of former politician and journalist Radek John in an investigative programme Bez cenzury on TV Barrandov. Even though John was a politician and leader of Czech political party Věci veřejné, he decided to return to journalism. He did so with no intention of resigning his chair in the Chamber of Deputies, part of the Parliament of the Czech Republic. With this in mind, this could be considered as a specific representation of political parallelism. This study describes specific cases where Radek John might have acted contrary to normative journalistic values and journalism ethics. Furthermore, the thesis analyses the possibility that Radek John's own political interests and opinions affected the television programme itself. The research is conducted using the method of a case study which combines qualitative and quantitative content analysis, qualitative interviews and other research methods. Consequently, the analysis found signs of biased reporting in the programme's introduction and some stories, which reflected Radek John's antipathy towards traditional political parties and their leaders. However, the research did not reveal any direct support of Věci veřejné or their representatives. Furthermore, the thesis also presents media, academic and political responses to..

Minutes, October 18, 1906

Originally published in Biomedical Optics Express on 01 March 2015 (boe-6-3-736

Visualization 6

User interaction-based orthographic-view images: object 3

Visualization 5: Integral imaging microscopy with enhanced depth-of-field using a spatial multiplexing

Visualization 5 Originally published in Optics Express on 08 February 2016 (oe-24-3-2072

Visualization 4

User interaction-based orthographic-view images: object 1