Addiction-related genes in gambling disorders:new insights from parallel human and pre-clinical models

Neurobiological research supports the characterization of disordered gambling (DG) as a behavioral addiction. Recently, an animal model of gambling behavior was developed (rat gambling task, rGT), expanding the available tools to investigate DG neurobiology. We investigated whether rGT performance and associated risk gene expression in the rat's brain could provide cross-translational understanding of the neuromolecular mechanisms of addiction in DG. We genotyped tagSNPs (single-nucleotide polymorphisms) in 38 addiction-related genes in 400 DG and 345 non-DG subjects. Genes with P<0.1 in the human association analyses were selected to be investigated in the animal arm to determine whether their mRNA expression in rats was associated with the rat's performance on the rGT. In humans, DG was significantly associated with tagSNPs in DRD3 (rs167771) and CAMK2D (rs3815072). Our results suggest that age and gender might moderate the association between CAMK2D and DG. Moderation effects could not be investigated due to sample power. In the animal arm, only the association between rGT performance and Drd3 expression remained significant after Bonferroni correction for 59 brain regions. As male rats were used, gender effects could not be investigated. Our results corroborate previous findings reporting the involvement of DRD3 receptor in addictions. To our knowledge, the use of human genetics, pre-clinical models and gene expression as a cross-translation paradigm has not previously been attempted in the field of addictions. The cross-validation of human findings in animal models is crucial for improving the translation of basic research into clinical treatments, which could accelerate neurobiological and pharmacological investigations in addictions

Efeitos da adubação, configuração de plantio e cultivares na cultura do gergelim no nordeste do Brasil.

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Influência do espaçamento e da densidade de plantio no rendimento da cultivar CNPA Precoce 1.

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Comparação entre indicadores agroeconômicos de avaliação de agroecossistemas consorciados e solteiros envolvendo algodão upland e feijão caupi.

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Método prático para separar os efeitos da competição interespecífica e da população de plantas em estudos de sistemas consorciados.

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Controle integrado de plantas daninhas em agroecossistemas cotonicolas.

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Dosagens de Diuron e Trifluoralina em algodoeiro herbaceo municipio de Iguatu, Ceara.

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Estimativa da energia cultural na cotonicultura arbórea no Nordeste brasileiro, comparando-se o Mocó tradicional com o precoce.

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Effect of the environment on zinc and iron levels in common beans.

To characterize Zn and Fe high content promising bean lines concerning genotype x environment (GxE) interaction, those mineral contents have to be evaluated for a certain number of years in various locations and cropping seasons to get a reliable estimate of that interaction. That procedure would allow the identification of genotypes with high stability for those nutrient contents, giving more confidence to breeders when new biofortified cultivars are to be released