1,858 research outputs found
Effects of ethyl-esterization, chain-lengths, unsaturation degrees, and hyperthermia on carcinostatic effect of omega-hydroxylated fatty acids
Aim: To evaluate promotive effect of hyperthermia on the carcinostatic activity of synthesized omega-hydroxy fatty acids (wHFAs) and their ethylesters agaist Ehrlich ascites tumor (EAT) cells. Methods: EAT cells were cultured with either wHFAs or their ethylester derivatives in a water bath at either 37 Β°C or 42 Β°C for 30 min, followed by incubation in a CO2 incubator for 20 or 72 h. Mitochondrial dehydrogenase-based WST-1 assay and trypan blue dye exclusion assay were then conducted after incubation. Morphological changes were observed by scanning electron microscopy (SEM). Results: Omega-HFA having a saturated 16-carbon straight-chain (wH16:0) was the most carcinostatic (at 37 Β°C β viability level: 60.0%; at 42 Β°C β 49.6% (WST-1)) among saturated and unsaturated wHFAs with 12, 15 or 16 carbon atoms, when administrated to EAT cells at 100 Β΅M for 20 h. Carcinostatic activity was markedly enhanced by ethyl-esterization of saturated fatty acids, such as wH16:0 (at 37 Β°C β 42.3%; at 42 Β°C β 11.2% , ibid) and wH15:0 (at 37 Β°C β 74.6%; at 42 Β°C β 25.3% , ibid), and their unsaturated counterparts were extremely effective only in combination with hyperthermia. Prolongation of the incubation period to 72 h at the same concentration increased appreciably their carcinostatic effect (wH16:0 ethylesther: 1.3%; wH15:0 ethylesther: 8.0%). These values were also supported by dye exclusion assay. The carcinostatic activity enhanced more markedly by hyperthermia (1.2%; 2.1%, ibid). SEM shows that wH16:0 ethylester-exposed EAT cells underwent extensive injury, such as deformation of cell structure or disappearance of microvilli. Conclusions: wH16:0 ethylester possesses high carcinostatic activity in vitro in combination with hyperthermia and may be utilized as potent anticancer therapeutic agent.Π¦Π΅Π»Ρ: ΠΏΡΠΎΠ°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°ΡΡ ΡΡΠΈΠ»ΠΈΠ²Π°ΡΡΠΈΠΉ ΡΡΡΠ΅ΠΊΡ Π³ΠΈΠΏΠ΅ΡΡΠ΅ΡΠΌΠΈΠΈ Π½Π° ΠΊΠ°Π½ΡΠ΅ΡΠΎΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΎΠΌΠ΅Π³Π°Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΆΠΈΡΠ½ΡΡ
ΠΊΠΈΡΠ»ΠΎΡ (HFAs) ΠΈ ΠΈΡ
ΡΡΠΈΠ»ΠΎΠ²ΡΡ
ΡΡΠΈΡΠΎΠ² ΠΏΠΎ ΠΎΡΠ½ΠΎΠ΅Π½ΠΈΡ ΠΊ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌ Π°ΡΡΠΈΡΠ½ΠΎΠΉ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΡΠ»ΠΈΡ
Π°
(EAT). ΠΠ΅ΡΠΎΠ΄Ρ: ΠΊΠ»Π΅ΡΠΊΠΈ EAT ΠΈΠ½ΠΊΡΠ±ΠΈΡΠΎΠ²Π°Π»ΠΈ Ρ HFAs ΠΈΠ»ΠΈ ΠΈΡ
ΡΡΠΈΠ»ΡΡΠΈΡΠ½ΡΠΌΠΈ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΠΌΠΈ Π½Π° Π²ΠΎΠ΄Π½ΠΎΠΉ Π°Π½Π΅ ΠΏΡΠΈ 37 Β° ΠΈΠ»ΠΈ
42 Β° Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 30 ΠΌΠΈΠ½ Ρ Π΄Π°Π»ΡΠ½Π΅ΠΉΠΈΠΌ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π² 2
ΠΈΠ½ΠΊΡΠ±Π°ΡΠΎΡΠ΅ Π½Π° ΠΏΡΠΎΡΡΠΆΠ΅Π½ΠΈΠΈ 20 ΠΈΠ»ΠΈ 72 Ρ, ΠΏΠΎΡΠ»Π΅ ΡΠ΅Π³ΠΎ Π°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π»ΠΈ
ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ Π°Π½Π°Π»ΠΈΠ·Π° WST-1, ΠΎΡΠ½ΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Π½Π° Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΡΡ
Π΄Π΅Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π½Π°Π·, ΠΈ ΠΏΠΎ
Π²ΠΊΠ»ΡΡΠ΅Π½ΠΈΡ ΡΡΠΈΠΏΠ°Π½ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΈΠ½Π΅Π³ΠΎ. ΠΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠΊΠ°Π½ΠΈΡΡΡΡΠ΅ΠΉ ΡΠ»Π΅ΠΊΡΡΠΎΠ½Π½ΠΎΠΉ
ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: ΠΏΡΠΈ ΠΊΡΠ»ΡΡΠΈΠ²Π°ΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ EAT Π² ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠΈ 100 M ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 20 Ρ ΠΎΠΌΠ΅Π³Π°-HFA
Ρ Π½Π°ΡΡΡΠ΅Π½Π½ΠΎΠΉ 16-ΡΠ³Π»Π΅ΡΠΎΠ΄Π½ΠΎΠΉ ΠΏΡΡΠΌΠΎΠΉ ΡΠ΅ΠΏΡΡ (H16:0) ΠΏΡΠΎΡΠ²Π»ΡΠ»ΠΈ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΡΠΉ ΠΊΠ°Π½ΡΠ΅ΡΠΎΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΡΡΠ΅ΠΊΡ (ΠΏΡΠΈ
37 Β° ΡΡΠΎΠ²Π΅Π½Ρ ΠΆΠΈΠ·Π½Π΅ΡΠΏΠΎΡΠΎΠ½ΠΎΡΡΠΈ ΡΠΎΡΡΠ°Π²ΠΈΠ» 60,0%; ΠΏΡΠΈ 42 Β° 49,6% (WST-1)) ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΡΠ°ΠΊΠΎΠ²ΡΠΌ Π½Π°ΡΡΡΠ΅Π½Π½ΡΡ
ΠΈ Π½Π΅Π½Π°ΡΡΡΠ΅Π½Π½ΡΡ
HFAs, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ
12, 15 ΠΈΠ»ΠΈ 16 Π°ΡΠΎΠΌΠΎΠ² ΡΠ³Π»Π΅ΡΠΎΠ΄Π°. Π°Π½ΡΠ΅ΡΠΎΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠ°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ Π²ΠΎΠ·ΡΠ°ΡΡΠ°Π»Π° ΠΏΡΠΈ
ΡΡΠΈΠ»ΡΡΠ΅ΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Π½Π°ΡΡΡΠ΅Π½Π½ΡΡ
ΠΆΠΈΡΠ½ΡΡ
ΠΊΠΈΡΠ»ΠΎΡ, ΡΠ°ΠΊΠΈΡ
ΠΊΠ°ΠΊ H16:0 (ΠΏΡΠΈ 37 Β° 42,3%; ΠΏΡΠΈ 42 Β° 11,2%, ibid) ΠΈ H15:0
(ΠΏΡΠΈ 37 Β° 74,6%; ΠΏΡΠΈ 42 Β° 25,3% , ibid), Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΠ΅ Π½Π΅Π½Π°ΡΡΡΠ΅Π½Π½ΡΡ
ΠΊΠΈΡΠ»ΠΎΡ Π±ΡΠ»ΠΈ Π²ΡΡΠΎΠΊΠΎΡΡΡΠ΅ΠΊΡΠΈΠ²Π½Ρ
ΡΠΎΠ»ΡΠΊΠΎ Π² ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Ρ Π³ΠΈΠΏΠ΅ΡΡΠ΅ΡΠΌΠΈΠ΅ΠΉ. Π£Π²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΏΠ΅ΡΠΈΠΎΠ΄Π° ΠΈΠ½ΠΊΡΠ±Π°ΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ Π΄ΠΎ 72 Ρ ΠΏΡΠΈ ΡΠΎΠΉ ΠΆΠ΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ Π²Π΅ΡΠ΅ΡΡΠ²
ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»ΠΎ ΠΊ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌΡ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ ΠΈΡ
ΠΊΠ°Π½ΡΠ΅ΡΠΎΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ (ΡΡΠΈΠ»ΠΎΠ²ΡΠΉ ΡΡΠΈΡ H16:0 1,3%; ΡΡΠΈΠ»ΠΎΠ²ΡΠΉ ΡΡΠΈΡ
H15:0 ethylesther 8,0%), ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½Π½ΠΎΠ³ΠΎ Π΄Π°Π½Π½ΡΠΌΠΈ ΠΎΠΊΡΠ°ΡΠΊΠΈ ΡΡΠΈΠΏΠ°Π½ΠΎΠ²ΡΠΌ ΡΠΈΠ½ΠΈΠΌ. ΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π³ΠΈΠΏΠ΅ΡΡΠ΅ΡΠΌΠΈΠΈ ΡΠ°ΠΊΠΆΠ΅ ΡΡΠΈΠ»ΠΈΠ²Π°Π»ΠΎ
ΠΊΠ°Π½ΡΠ΅ΡΠΎΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ (1,2%; 2,1%, ibid). Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ SEM ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ, ΡΡΠΎ
ΠΊΠ»Π΅ΡΠΊΠΈ EAT, ΠΈΠ½ΠΊΡΠ±ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ Ρ ΡΡΠΈΠ»ΠΎΠ²ΡΠΌ ΡΡΠΈΡΠΎΠΌ H16:0, ΡΠ°Π·ΡΡΠ°ΡΡΡΡ Ρ Π½Π°ΡΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΡΡΡΡΠΊΡΡΡΡ ΠΈ ΠΈΡΡΠ΅Π·Π½ΠΎΠ²Π΅Π½ΠΈΠ΅ΠΌ
ΠΌΠΈΠΊΡΠΎΠ²ΠΎΠ»ΠΎΠΊΠΎΠ½. ΠΡΠ²ΠΎΠ΄Ρ: Π² ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Ρ Π³ΠΈΠΏΠ΅ΡΡΠ΅ΡΠΌΠΈΠ΅ΠΉ ΡΡΠΈΠ»ΠΎΠ²ΡΠΉ ΡΡΠΈΡ H16:0 ΠΏΡΠΎ Π΅Ρ Π²ΡΡΠΎΠΊΡΡ ΠΊΠ°Π½ΡΠ΅ΡΠΎΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΡΡ
Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ in vitro, ΡΡΠΎ Π³ΠΎΠ²ΠΎΡΠΈΡ ΠΎ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΡ Π² ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ
Evolution of changes in the computed tomography scans of the brain of a patient with left middle cerebral artery infarction: a case report
<p>Abstract</p> <p>Introduction</p> <p>Stroke is a common and important condition in medicine. Effective early management of acute stroke can reduce morbidity and mortality.</p> <p>Case presentation</p> <p>A 63-year-old man presented to the Accident and Emergency department with a history of collapse and progressive right-sided weakness. Clinically this was a cerebrovascular accident affecting the left hemisphere of the brain causing right hemiplegia. Computed tomography scans, performed 3 days apart, showed the evolution of infarction in the brain caused by the thrombus in the left middle cerebral artery. This is one of the early signs for stroke seen on computed tomography imaging and it is called the hyperdense middle cerebral artery sign.</p> <p>Conclusion</p> <p>Patients admitted with a stroke, undergo CT brain within 24 hours. The scan usually takes place at admission into the hospital and is done to rule out a bleed or a space occupying lesion within the brain. A normal CT brain does not confirm a stroke has not taken place. When scanned early, the changes seen on the CT due to an infarction from a thrombus may not have taken place yet. This paper highlights the early changes that can be seen on the CT brain following a stroke caused by infarction due to a thrombus in the middle cerebral artery.</p
Neutral B Flavor Tagging for the Measurement of Mixing-induced CP Violation at Belle
We describe a flavor tagging algorithm used in measurements of the CP
violation parameter sin2phi_1 at the Belle experiment. Efficiencies and wrong
tag fractions are evaluated using flavor-specific B meson decays into hadronic
and semileptonic modes. We achieve a total effective efficiency of $ 28.8 +-
0.6 %.Comment: 28 pages, 9 figure
Computational modeling of beam-customization devices for heavy-charged-particle radiotherapy
A model for beam customization with collimators and a range-compensating
filter based on the phase-space theory for beam transport is presented for dose
distribution calculation in treatment planning of radiotherapy with protons and
heavier ions. Independent handling of pencil beams in conventional pencil-beam
algorithms causes unphysical collimator-height dependence in the middle of
large fields, which is resolved by the framework comprised of generation,
transport, collimation, regeneration, range-compensation, and edge-sharpening
processes with a matrix of pencil beams. The model was verified to be
consistent with measurement and analytic estimation at a submillimeter level in
penumbra of individual collimators with a combinational-collimated carbon-ion
beam. The model computation is fast, accurate, and readily applicable to
pencil-beam algorithms in treatment planning with capability of combinational
collimation to make best use of the beam-customization devices.Comment: 16 pages, 5 figure
Abnormalities in Osteoclastogenesis and Decreased Tumorigenesis in Mice Deficient for Ovarian Cancer G Protein-Coupled Receptor 1
Ovarian cancer G protein-coupled receptor 1 (OGR1) has been shown to be a proton sensing receptor in vitro. We have shown that OGR1 functions as a tumor metastasis suppressor gene when it is over-expressed in human prostate cancer cells in vivo. To examine the physiological functions of OGR1, we generated conditional OGR1 deficient mice by homologous recombination. OGR1 deficient mice were viable and upon gross-inspection appeared normal. Consistent with in vitro studies showing that OGR1 is involved in osteoclastogenesis, reduced osteoclasts were detected in OGR1 deficient mice. A pH-dependent osteoclasts survival effect was also observed. However, overall abnormality in the bones of these animals was not observed. In addition, melanoma cell tumorigenesis was significantly inhibited in OGR1 deficient mice. OGR1 deficient mice in the mixed background produced significantly less peritoneal macrophages when stimulated with thioglycolate. These macrophages also showed altered extracellular signal-regulated kinases (ERK) activation and nitric oxide (NO) production in response to lipopolysaccharide. OGR1-dependent pH responses assessed by cAMP production and cell survival in macrophages or brown fat cells were not observed, presumably due to the presence of other proton sensing receptors in these cells. Our results indicate that OGR1's role in osteoclastogenesis is not strong enough to affect overall bone development and its role in tumorigenesis warrants further investigation. The mice generated can be potentially used for several disease models, including cancers or osteoclast-related diseases
Search for the Lepton-Flavor-Violating Decay at Belle
We have searched for the Lepton Flavor Violating decay using a data sample of 84.3 fb accumulated with the Belle detector
at KEK. The -meson was detected through the decay modes: and . No signal candidates are found, and we
obtain an upper limit for the branching fraction at the 90% confidence level.Comment: Submitted to Phys.Rev.Let
Observation of the DsJ(2317) and DsJ(2457) in B decays
We report the first observation of the B --> Dbar DsJ(2317) and B --> Dbar
DsJ(2457) decays based on 123.8 10^6 BBar events collected with the Belle
detector at KEKB. We observe the DsJ(2317) decay to Ds pi0 and DsJ(2457) decay
to the Ds* pi0 and Ds gamma final states. We also set 90% CL upper limits for
the decays DsJ(2317) --> Ds* gamma, DsJ(2457) --> Ds* gamma, DsJ(2457) --> Ds
pi0 and DsJ(2457) --> Ds pi+ pi-.Comment: 6 pages, 3 figures. A few minor corrections. Replaced by version
accepted to publication in Phys. Rev. Let
Observation of a narrow charmonium-like state in exclusive B+ -> K+ pi+pi- J/psi decays
We report the observation of a narrow charmonium-like state produced in the
exclusive decay process B+ -> K+ pi+pi- J/psi. This state, which decays into
pi+pi- J/psi, has a mass of 3872.0+-0.6(stat)+-0.5(syst) MeV, a value that is
very near the M_D + M_D* mass threshold. The results are based on an analysis
of 152M B-Bbar events collected at the Upsilon(4S) resonance in the Belle
detector at the KEKB collider. The statistical significance of the signal is in
excess of 10 sigma.Comment: 10 pages 4 figures, submitted to Physical Review Letter
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