Study of SREBP1 and SERBP2 in peripheral blood mononuclear cells fron hypercholesterolemic rabbits

Peripheral blood mononuclear cells are a possible biomarker that could reveal molecular alterations before the development of the disease. Therefore, the objective of the present investigation is to study molecular and genetic changes that indicate metabolic modifications even with normal biochemical values. SERBP1 and SERBP2 are proteins involved in lipid metabolic. These molecules can be expressed in peripheral blood mononuclear cells. This allows study tissue changes without resorting to biopsies. In this study, one control group of New Zealand rabbits was fed with a balanced feed (C) and another group received the same balanced feed supplemented with 17% fat (F). These animals did not receive fructose overload, maintaining constant concentrations of carbohydrates and protein in both groups. In biochemical tests from both groups were observed similar levels of glucose (C group: 140.7 + 28.4 mg/dl / F group: 118.3 +12.0 mg/dl) and triglyceride (C group: 144.1 +15.5 mg/dl / F group: 135.6 +8.3 mg/dl), while F group showed increased levels of cholesterol (42.8 +21.6mg/dl) compared with C group (27.1 +4.5 mg/dl). However, there is variability in the cholesterol values because some animals of the F group do not experience significant increment despite the intake of fat. This interesting finding leads to the hypothesis that changes in lipid metabolism can be examined by the expression of different genes early. As preliminary results, we observed by immunohistochemistry the presence nuclear of SREBP1 and SREBP2 in lymphocytes of the F group, while in C group was not observed immunoreaction or cytoplasmic signal. This result is indicating an activation of the lipid metabolism before being able to observe changes at a biochemical level.Fil: Saez, Aldana. Universidad "Juan Agustín Maza"; ArgentinaFil: Mussi, Jesica. Universidad "Juan Agustín Maza"; ArgentinaFil: Funes, Abi Karenina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Colombo, Regina Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Fornes, Miguel Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Saez Lancellotti, Tania Emilce Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Boarelli, P. Universidad "Juan Agustín Maza"; ArgentinaXXXVI Reunión Científica Anual de la Sociedad de Biología de CuyoMendozaArgentinaSociedad Argentina de Biologí

Efecto de la dieta hiperlipemica sin suplementación de carbohidratos sobre el tejido hepático en conejos adultos neozelandeses

Las dislipidemias adquiridas se asocian a diversos trastornos metabólicos como diabetes mellitus, obesidad y síndrome metabólico. Si bien en algunos casos se conocen los mecanismos que los generan aún no está claro el de algunas de ellas. El objetivo de la presente investigación fue desarrollar un modelo experimental de dislipidemia inducida por una dieta lipídica sin carbohidratos añadidos. Esto nos permite analizar parámetros bioquímicos y tisulares sin generar un modelo de Síndrome Metabólico (SM). El modelo animal, conejos machos adultos de Nueva Zelanda, permite evaluar los cambios en la dieta a lo largo del tiempo. Los animales fueron alimentados durante 12 meses en cuatro trimestres (I, II, III y IV). En I, los animales fueron alimentados con una dieta estándar para conejos. En II, se añadió N: 7 14% de grasa bovina (G) y N: 2 con 14% de aceite de oliva (AO). N:2 siguieron con alimentación estándar (grupo C). En III, N: 3 comenzó a consumir solo el 7% de G (MG) y N: 4 continuó con G hasta el final del experimento. En IV el MG consumió 4% de G con 4% de AO (MGAO)

A LOV Protein Modulates the Physiological Attributes of Xanthomonas axonopodis pv. citri Relevant for Host Plant Colonization

Recent studies have demonstrated that an appropriate light environment is required for the establishment of efficient vegetal resistance responses in several plant-pathogen interactions. The photoreceptors implicated in such responses are mainly those belonging to the phytochrome family. Data obtained from bacterial genome sequences revealed the presence of photosensory proteins of the BLUF (Blue Light sensing Using FAD), LOV (Light, Oxygen, Voltage) and phytochrome families with no known functions. Xanthomonas axonopodis pv. citri is a Gram-negative bacterium responsible for citrus canker. The in silico analysis of the X. axonopodis pv. citri genome sequence revealed the presence of a gene encoding a putative LOV photoreceptor, in addition to two genes encoding BLUF proteins. This suggests that blue light sensing could play a role in X. axonopodis pv. citri physiology. We obtained the recombinant Xac-LOV protein by expression in Escherichia coli and performed a spectroscopic analysis of the purified protein, which demonstrated that it has a canonical LOV photochemistry. We also constructed a mutant strain of X. axonopodis pv. citri lacking the LOV protein and found that the loss of this protein altered bacterial motility, exopolysaccharide production and biofilm formation. Moreover, we observed that the adhesion of the mutant strain to abiotic and biotic surfaces was significantly diminished compared to the wild-type. Finally, inoculation of orange (Citrus sinensis) leaves with the mutant strain of X. axonopodis pv. citri resulted in marked differences in the development of symptoms in plant tissues relative to the wild-type, suggesting a role for the Xac-LOV protein in the pathogenic process. Altogether, these results suggest the novel involvement of a photosensory system in the regulation of physiological attributes of a phytopathogenic bacterium. A functional blue light receptor in Xanthomonas spp. has been described for the first time, showing an important role in virulence during citrus canker disease