Reconciling American Marijuana Policy in a Federal System

The recent successful ballot initiatives in Colorado and Washington to legalize recreational marijuana despite restrictive federal law continue to demonstrate the disconnect between national and state marijuana policy. In order to understand how many of these national policies were enacted, an investigation will be presented of the discriminatory history of marijuana legislation, indicating the inconsistent nature of past regulation. Thus following will be an examination of relevant Supreme Court cases depicting the Supreme Court’s ultimate hesitation to prevent the states from circumventing federal marijuana law. Finally, a discussion will be held on the ramifications of inconsistent state and national policies, which create a system that is at odds with the principles of the Constitution. In the face of growing public support for medical marijuana and increased state action towards allowing more access to the drug for medical and even recreational purposes, the federal government should reexamine its policies in order to maintain a healthy democracy

Who\u27s Your Daddy? A Study of Extra-Pair Copulation and Mating Behaviors of Protonotaria citrea

Mating behavior has a profound impact on reproductive success and the resulting genetic structure of offspring. Extra-pair copulation is a widely observed behavior within avian species. This study explored the genetic effects of mating behaviors of Prothonotary warblers, Protonotaria citrea (Parulidae), using co-dominant microsatellite markers. Prothonotary warblers are migratory songbirds that build nests in cavities, commonly found in wetland habitats. A set of artificial nest boxes were initiated by Dr Robert Reilly in 2002 in Dutch Gap, Chesterfield County Virginia, USA, a tidal tributary off the James River. From this population, 28 nest boxes were surveyed yielding 47 adults and 110 offspring. All individuals were genotyped and the multilocus genotypes were used to identify parentage. Using paternity exclusion, 27.2% of offspring were identified as resulting from extra-pair mating. Surprisingly, an additional 11.8% of offspring were classified as genetically unrelated to the resident female, being presumably the result of an egg dumping from a female not caught at the nest box. The vast majority of nest boxes, 82.1% in this study, had at least one offspring that was a result of a mating behavior outside of the social pair. There was also positive spatial autocorrelation in extra-pair paternity suggesting that these matings are not randomly distributed across the sampling landscape. These data show that Protonotaria citrea engage in both extra-pair copulations and nest parasitism. Whether this is normal mating behavior for the species, or something that is unique to this population is unknown

Temporal separation of aggregation and ubiquitination during early inclusion formation in transgenic mice carrying the Huntington's disease mutation.

Abnormal insoluble ubiqitinated protein aggregates are found in the brains of Huntington's disease (HD) patients and in mice transgenic for the HTT mutation. Here, we describe the earliest stages of visible NII formation in brains of R6/2 mice killed between 2 and 6 weeks of age. We found that huntingtin-positive aggregates formed rapidly (within 24-48 hours) in a spatiotemporal manner similar to that we described previously for ubiquitinated inclusions. However, in most neurons, aggregates are not ubiquitinated when they first form. It has always been assumed that mutant huntingtin is recognised as 'foreign' and consequently ubiquitinated and targeted for degradation by the ubiquitin-proteasome system pathway. Our data, however, suggest that aggregation and ubiquitination are separate processes, and that mutant huntingtin fragment is not recognized as 'abnormal' by the ubiquitin-proteasome system before aggregation. Rather, mutant Htt appears to aggregate before it is ubiquitinated, and then either aggregated huntingtin is ubiquitinated or ubiquitinated proteins are recruited into aggregates. Our findings have significant implications for the role of the ubiquitin-proteasome system in the formation of aggregates, as they suggest that this system is not involved until after the first aggregates form

Exploring and assessing social research impact : a case study of a research partnership’s impacts on policy and practice

There is increasing emphasis on the outcomes of research in terms of its impact on wider society. However in the social sciences the ways in which research is taken up and used, discussed, shared and applied in different policy, practice and wider settings is complex. This thesis set out to investigate the ways in which social research was used by various non-academic actors, and to explore what impact it had in order to develop methods for understanding and assessing impact. The research investigated what research impact is, how it occurs, and how it might be assessed. The research was in two phases: firstly, a case study of a research partnership between a research centre and a voluntary organisation; and, secondly, the development and seeking feedback on a framework to assess impact. The care study employed two main approaches: forward-tracking - from research to policy and/or practice - and backward tracking - from policy back to research. Both phases were conducted through a practitioner-research approach, bringing experience of working with the projects involved into the heart of the research model. The study found many ways the research from the partnership had been used in different sectors by different actors. Impacts from the research were harder to identify. In cases where there were clear impacts, the actors involved had adapted research to fit the context for research use in order to create impact. Research users continued to draw on the research for many years after publication, creating further impact as new policy or practice agendas arose. The framework used a 'pathways to impact' model to develop a theory-based approach to assessing impact and to create categories for data collection. The ways in which research might impact on policy and practice are many and cannot be easily predicted. Concepts from complexity theory, particularly a focus on relationships, an understanding of context and the concept of emergence have been useful in framing the picture of impact generated from this research. Any assessment of impact from social research needs to acknowledge that many actors are involved in the process of research being taken up and used, and impact cannot be achieved from the supply side alone. Partnership research, between an academic and voluntary sector organisation, facilitated the use and impact of the research in many ways. The thesis reconceptualises ideas about how research impacts on society, suggesting the concept of 'contribution' is more accurate and useful than attribution. It also adds to the body of empirical work on the processes of impact, and in particular of the role of research partnerships in increasing impact. It suggests that process-based approaches to assessing impact that acknowledge complexity may be fruitful in developing impact assessment methodology

The Health Sciences and Technology Academy: Re-Imagining Programmatic Delivery During the COVID-19 Pandemic

Out-of-school time academic/STEM programs provide educational enrichment to a myriad of student populations with some designed to assist those underrepresented and at-risk who desire to purse post-secondary studies. One such program in West Virginia (WV) is the Health Sciences and Technology Academy (HSTA), which provides hands-on, in person and engaging educational enrichment with the intent to increase the college going rates of Appalachia’s most vulnerable youth. In March 2020, HSTA key personnel encountered the task of redesigning program delivery due to the immediate shutdown of all in person operations resulting from the novel coronavirus, SARS-CoV-19 (COVID-19) pandemic. This paper discusses the programmatic changes of program delivery during HSTA’s key program components—the Summer Institute and the Community Based Programming. This paper also utilizes repeated measures one-way multivariate analysis (MANOVA) and paired t-test analysis to examine participants’ perspectives on learning, enjoyment and satisfaction of the aforementioned components prior to and during the COVID-19 pandemic

A novel and practical care process framework to inform model of care development

Breaking free of pre-existing assumptions to achieve transformative change in care delivery remains challenging. This paper presents a care process framework (CPF) using a Rapid Task Analysis (RTA) tested with healthcare teams across five communities in British Columbia, Canada, to provide leaders a novel and practical approach to care model development. The study's goals were to determine if the care process framework was replicable even though the population care needs differed for each community. The results showed the framework was replicable, informed the care model development and identified ideal scopes of practice and team composition given the context of care. The framework also captured expert tacit knowledge and decision-making to build capacity given our current workforce challenges. For operational leaders and government agencies, the use of the framework may influence a shift in historical approaches that better aligns health and human resources capacity to population health and service needs

Tensor-based morphometry and stereology reveal brain pathology in the complexin1 knockout mouse.

Complexins (Cplxs) are small, soluble, regulatory proteins that bind reversibly to the SNARE complex and modulate synaptic vesicle release. Cplx1 knockout mice (Cplx1(-/-)) have the earliest known onset of ataxia seen in a mouse model, although hitherto no histopathology has been described in these mice. Nevertheless, the profound neurological phenotype displayed by Cplx1(-/-) mutants suggests that significant functional abnormalities must be present in these animals. In this study, MRI was used to automatically detect regions where structural differences were not obvious when using a traditional histological approach. Tensor-based morphometry of Cplx1(-/-) mouse brains showed selective volume loss from the thalamus and cerebellum. Stereological analysis of Cplx1(-/-) and Cplx1(+/+) mice brain slices confirmed the volume loss in the thalamus as well as loss in some lobules of the cerebellum. Finally, stereology was used to show that there was loss of cerebellar granule cells in Cplx1(-/-) mice when compared to Cplx1(+/+) animals. Our study is the first to describe pathological changes in Cplx1(-/-) mouse brain. We suggest that the ataxia in Cplx1(-/-) mice is likely to be due to pathological changes in both cerebellum and thalamus. Reduced levels of Cplx proteins have been reported in brains of patients with neurodegenerative diseases. Therefore, understanding the effects of Cplx depletion in brains from Cplx1(-/-) mice may also shed light on the mechanisms underlying pathophysiology in disorders in which loss of Cplx1 occurs

Diabetes Knowledge, Behaviors, and Perceptions of Risk in Rural West Virginia Counties

Introduction: A little less than half of American adults have diabetes or pre-diabetes. In 2016, West Virginia (WV) had the highest percentage (15.2%) of adults with diagnosed diabetes in the U.S. Purpose: In partnership with the Health Sciences and Technology Academy (HSTA), a cross-sectional study was preformed to assess knowledge, behaviors, and perceptions of diabetes risk. Methods: Data was collected by trained HSTA students and teachers who lived in rural counties in WV. Information was assessed using validated surveys, and HbA1c was obtained by utilizing professional point-of-care (Bayer) kits. Results: Mean age and Body Mass Index (BMI) was 36.11±17.86 years and 27.80±6.09 kg/m2, respectively. More than half of the participants had a family history of diabetes (58.8%) and hypertension (60.2%), and a majority had elevated BMI (65.9%). However, only 29.2% rated their future risk for diabetes as moderate to high. Eighty percent (80%) had an inadequate amount of weekly exercise, and 36% had lower quality of diet. Overall, dietary quality and diabetes knowledge was associated with a low to moderate diabetes risk score; risk score positively correlated with higher HbA1c (r=0.439, P\u3c.001). Participants’ HbA1c, perceived future risk of diabetes and family history of diabetes emerged as significant predictors of diabetes risk in the regression model, controlling for health behavior and diabetes knowledge. Implications: HbA1c, perceived future risk of diabetes and family history of diabetes may be the best predictors of developing diabetes in the future and, therefore, are important to assess during community screening. Perception of diabetes risk is lower than actual diabetes risk in WV

High School Students’ Learning During the COVID Pandemic: Perspectives from Health Sciences and Technology Academy Participants

This paper examines the perspectives of Health Sciences and Technology Academy (HSTA) participants as they navigate through their West Virginia (WV) high school learning environments (i.e., in-person, blended/hybrid, complete virtual) during the novel coronavirus, SARS-CoV-19 (COVID-19) pandemic. In March of 2020, the participants in this out-of-school-time (OST) academic enrichment program for exceptionally driven, yet underprivileged, at-risk students, with over 70% living in rural areas, started receiving remote learning instruction through learning management systems or via paper packets. In August of 2020, school systems provided parents and caregivers alternative learning environments for their student(s). In order to understand the learning experiences of HSTA students during these unprecedented times, HSTA released the 2020 Learning Outcomes Survey to participants in December of 2020. We performed chi-square test of independence to test the relationship between participants’ learning environments, their satisfaction with the education they were receiving, their ability to keep up with their school-work, teacher availability to help when needed and teacher feedback supporting them in their learning environment. The results show significant differences between the learning environments and keeping up with school-work as well as teacher feedback supporting them in their learning; however, Phi and Cramer’s V tests for effect size show weak correlations. This study provides a small glimpse into HSTA students’ learning experiences as they attempted to continue to learn in their regular school environment during the COVID-19 pandemic while in HSTA