Infection of some species of fresh water fishes to parasitic worms in Khouzestan Province, Iran

During a survey on parasitic worms infection of local freshwater fishes in years 1994-95 in Khouzestan province wetlands, 206 individuals of fishes (62 individuals of Barbus luteus Heckel, 1843; 30 individuals of Cyprinus carpio L.; 6 individuals of Silirus triostegus Heckel, 1843; 92 individuals of Liza abu Heckel, 1843 and 15 individuals of Aspius vorax Heckel, 1843 of Hoor-al-azim and Hoor-shadegan were catched and they were examined. One of 62 B. luteus and two of 3 C. carpio were infected to Bothriocephalus opsariichthys Yamaguti, 1943 and two of 6 S. triostegus were infected to proteocephalus sp. Weinland, 1858. In this study, frequency of infection in B. luteus and C. carpio to B. opsariichthys was 1.6% and 6.6% respectively, and in S. triostegus was 33.3% to proteocephalus sp. One of 15 A. vorax and 51 of 93 L. abu were infected to Neoechinorhynchus tylosuri. In this study female and male parasites were collected from fish intestine. Frequency of infection in A. vorax and L abu were 6.6% and 54.8% respectively. This is the first record of N. tylosuri infection in A. vorax in Iran

Identification of myxozoa and protozoa parasites of Barboid fishes of water resources in Khouzestan Province

A survey on parasitic infection of Barboid fishes of Khouzestan Province in southwest Iran was conducted from spring 2002 to autumn 2004. A total of 296 specimens from five fish species were examined. The specimens were collected from five stations including Ahwaz, Golestan, Mollasani and Dez Reservoir on Karoun River and Hamidieh Reservoir and Shadegan Lagoon on Karkheh River. The fishes were transported alive to the laboratory where they measured and weighed and then killed by cutting their spinal cord. Eleven parasites were separated of which seven were identified to the species level and four to the genus level. Of the protozoans we found Ichlyophthirius multifiliis, Trichodina sp., Goussia v., Balantidium sp. and of the myxozoans we observed Myxobolus persicus, Mkaruni, M nodulointestinalis, Miranicus! Mmesopotamia, Myxobolus sp., Myxidium rhodei and Myxidium pfiefferi. We reported three new parasites and eleven new hosts for Iranian freshwater fishes and three new hosts for three Myzoxoans

Survey on alimentary tract parasites in cultured fish in Khouzestan province

In this study which was carried out in khoozestan province, 1100 pieces of common carp (Cyprinus carpio), silver carp (Hypophthalmichthys molitrix), Grass carp (Ctenopharyngodon idella) and Big head (Arysthychthys nobilis) from different parts of the province were examined. From each part, 10% of the fish farms were sampled according to the fish density at each farm. parasitic infection of gastrointestinal tract were studied on 600 pieces of silver carp (60% ) , 244 pieces of common carp (25%) , 51 pieces of Big head (5%) . only Grass carp was infested to protozoan and metazoan parasites . thirty percent of examined Grass carp of 2-730 gr weight were infested to Hexamita but infestation was more severe in Grass carp with 20-40 gr weight . macroscopic manifestation included : dysphagia , weakness , circulating movements, paling of gills , stomach inflammation , intestinal inflammation , ascitis and yellowish gut contents . in severe and intestinal necrosis along with hyperemia was apparent . the cestoda , bothriocephalus opsariichthydis ( Rudolphi , 1808 ) with an infestation rate of 35 % were only observed in Grass carp in 2-40 gr . Grass carp that showed severe infestation, intestinal blockade was observed with more than 100 parasites. The length of adult cestoda was 450-530 mm and its width was 5mm. in severe infestations, macroscopic manifestations included: ascitis, abdominal inflammation, swimming on water surface and emaciation. Histologic studies showed necrosis of intestinal wall, hemorrhage and localized necrosis. In fishes with severe infestations, inflammation was observed. Infestation with the nematode, capilaria was observed in 10% of 20-750 gr Grass carp. No macroscopic symptoms were identified in the samples, but microscopic examination showed acute necrosis along with hyperamia and submocusal hemorrhage. Lymphocytes were also observed. Adult nematode had a width of 0.075 and a length of 7.5-8.5 mm

Isolation and identification of bacterial and fungal microflora from Litopenaeus vannamei in Choibdeh, Abadan

Bacterial and fungal microflora of Litopenaeus vannamei cultured in Choibdeh, Abadan was studied. For this purpose, PLs before and after stocking and those shrimps persisting on food tray from June to August, 2006 were taken randomly. Live samples transferred to microbiology laboratory of South Aquaculture Research Center, Ahwaz. Special culture media (e.g. Tryptic Soy Agar + 1.5-2% Nacl & Sabouraud Dextrose Agar + 1.5-2% Nacl) were used for bacterial and fungal culture. We isolated 10 bacterial species of which Vibrio alginolyticus (36.92%) had high abundance among bacterial species. We also isolated and identified three fungal species including Aspergillus niger (66.66%) A. fumigatus (16.66%) and Fusarium sp. (16.66%). A. niger was predominant among fungal species. All bacterial and fungal species that were identified were opportunistic

Evaluation of the immunity factors (THC, TPP, PO, SOD, POD) of shrimp fed with the yeast Saccharomyces cerevisiae compared to shrimp fed without yeast

Effects of S. cerevisiae on immune parameters of the L. vannamei after 14 days of S .cerevisiae feeding were evaluated in this study. For this purpose a total of 300 pieces of shrimp with an average weight of 30 to 35 grams were selected from a pool shrimp Abadan CHOEBDEH. After making sure the health, absence of necrosis on the surface of the body, cuts antenna, shrimp were transferred to the center of BANDAR IMAM Research Station. Adaptation was carried out for 3-5 days. After the adaptation, shrimps were screened for virus (WSSV, TSV, MBV, HPV, YHV, BP, IHHNV and IMNV) and vibrio bacteria. After screening shrimps divided to two groups with three replication (including 50 pieces of shrimp in triplicate). The experimental diet has the commercial shrimp composition, but 2 g of S. cerevisiae substituted 2 g of fish meal. Shrimp of first group (T1) for 14 days with food containing nutritional yeast and shrimp in second group (T2) were fed with normal diet without yeast. After 14 days Immune Factors and survival rates in both groups were evaluated. The results showed that the relative survival rate between the two groups showed no significant difference. But Immune Factors (THC, TPP, PO, POD and SOD) in the treatment fed yeast (T1) compared to control treatment (T2) showed a significant increase. In conclusion these results suggest that the increased survival rate and resistance of shrimp after S. cerevisiae consumption occurs through immune modifications, such as increases in THC, TPP, SOD, SOP and PO activity

Evaluation of the immunity factors (THC, TPP, PO, SOD, POD) of shrimp fed with the algae Gracilaria corticata compared to shrimp fed without algae and exposed to white spot virus

White spot disease (WSD) is one of the shrimp deadliest viral diseases that causes heavy losses on all shrimp of Penaeid family. Most invertebrates are lacking adaptive immune system and its defense is the innate immune system that is as cellular and humoral, but a like immune system against white spot virus in shrimp was been detected. In this research, control and prevention of white spot disease in shrimp using algae Gracilaria corticata, investigated. About 300 vannamei shrimp were divided to 4 groups and fed by normal pellet and algae extract in 14 days. At the end of the fourteenth day half of the shrimp were challenged with acute white spot virus. After the fourteenth day in the days 0, 3, 9, 18 and 25 sampling were done from the hemolymph of survived shrimps and survival and immune factors were evaluated. Based on results, in the challenge test, shrimps that fed with algae extract has a significant survival rate than shrimp fed with commercial diet. Increased the Immune Factors from day one to day 25 observed during the test. Greatest amount of Immune Factors THC, TPP, SOD, POD and PO in T1 group were observed in day 25 of tests. This situation was also true for group2 (T2), but its rate significantly was less than group 1(T1)

Ecological study on rearing shrimp ponds (Litopenaeus vannemei) in Choebdeh Abadan

Following to introduce Vannamei shrimp (Litopenaeus vannamei) into Iran aquaculture industries by fisheries organization its postlarvae were successfully reared in ponds in Bushehr and Choebdeh in Khuzestan. Due to activity of Shrimp site and importance of input and output water quality, this study was done in 11 stations including: two stations in Bahmanshir river, two station in C4 and C5 irrigation canals, six staions in active farms along C4 cannel (Yonesi, Mosavei and Salman Zadeh), and C5 cannel (Mohamadi, Khairi and Ashraf Por) and one staion in output draing were selected. Sampling were carried out from May2009 befor beginning stocking to harvest time in October 2009. Physico- chemical parameters and plankton sampled biweekly and benthic animals and heavy metals were sampled monthly. Some parameters such as pH and temperature by using portable multy parameters and others. Analyzed with standard methods. Heavy metals were measured by plarography method (797 Vamtro). Plankton and benthos samples were studied only in Bahmanshir station. According to results the maximum values for DO (11.1 ppm) PO4 (1.86 ppm) NO3 (8.4 ppm) and TSS (4992 ppm), pH (8.4) NO2 (0.18 ppm) BOD5 (9.06 ppm) were measured. In comparison to aquaculture water quality standards except for TSS in river stations others are located in allowable range. We didn’t observed any main changes in water quality from river to output drainage. The results of biotic parameters in river stations showed that diatoms were the most group of phytoplanktons. Zooplankton groups were Copepods, Rotifera and Protozoa. Tintinids and Nematoda larvae were the most abundant zooplankton. Benthic animals were included nine crustacean species and two annelid species. Isopods with 52% were the most and ant group

Immunization of cultured shrimp against WSSV by use of produced recombinant vaccine (In vitro)

Shrimp diseases, especially viral diseases are the major limiting factors of shrimp farming activities in the world. White spot disease is one of the most pernicious viral disease that lead to serious loss in all shrimps of penaeids family. In Iran during 2002 to 2004 in Khuzestan province and in 2005 in Bushehr province, the most ponds and farms infected with white spot and the entire industry faced threat of closure. Surface proteins of virus has important role in the early stages of virus with host cell interaction, because these proteins usually enumerated as a candidate to produce a subunit and recombinant vaccines. On the other hand, these proteins can use as an important virus antigen and produce virus-specific antibodies in the preparation of diagnostic kits. In several studies proteins in the form of injection or oral vaccine has shown promising results. This study was designed in order to gain these proteins for immunization of white leg shrimp. To achieve the white spot virus genome, sampling and DNA extraction of moribund shrimps with clinical signs was done from Choebdeh farms. VP28 and VP19 protein-coding gene-specific primers were selected, and after amplification and purification were cloned in E.coli TG1. Protein expression evaluated and commercial plates were coated with inactivated bacteria containing the recombinant protein. White leg shrimp post larvae (PL30) were fed with recombinant food for 7 days then two times in 9th and 23th day after beginning of plate feeding and was challenged by white spot disease virus. The results of the first exposure (end of the seventh day after the shrimp fed with recombinant plate) showed that the lowest mortality at day 14 after exposure in different groups belong to VP28 group with cumulative mortality percent %30 ± 3.84 and the highest percentage of the Group TG1 equal to %72.22±2.93, also The highest and lowest relative percent survival, belong to the group of VP28 (60.29± 5.09%) and TG1 (4.41 ± 2.94%), respectively. In terms of mortality percent and relative percent survival, VP28 and VP 28+19 has significant difference with other groups while there was no significant difference between the groups VP19, TG1 and pMal. In the second exposure experiment (21 days after end of plate feeding) results showed that the lowest cumulative mortality in different groups belong to VP28 (%50 ± 5.09) and the highest percentage was owned by Group TG1 equal to %75.55±2.22, also the highest and the lowest of relative percent survival belong to VP28 (%32.86± 6.83) and TG1 (%0.00 ± 2.98) groups respectively. Similar to the first experiment, VP28 showed significant differences in cumulative mortality and relative percentage of survival in contrast to other group. As a general conclusion it can be noted that recombinant protein VP28 in any form that delivered to shrimp was able to protect shrimp against white spot virus. On the contrary VP19 has no this ability. The survival rate is directly related to the duration of the presence of recombinant protein in the diet

Preparation of inactivated vaccine to induce resistance against white spot syndrome virus by nuclear & non nuclear techniques

The inactivated of white spot syndrome virus (WSSV) by Gama and Electron radiation and chemical material such as Formalin with antigen property have been done during last year. At the first time the stock of virus prepared and purred it. Six hundred of shrimp Fenneropenaus indicus with average weight 7 to 12 gram collected from shrimp research station in Heleh area and transported to Shrimp Research Institute in Boushehr province. Vibrio parahemolyticus obtained from Veterinary University of Tehran and then lyophilized it. The bacterial V. parahemolyticus inactivated with Gamma, electron and formalin and then injected to shrimp for activated the immune system. The source of viruses was identified through PCR, TEM and histopathology methods from the shrimp infected in 2009 occurrence of boushehr province. The virus injected to crayfish and collected the hemlymph for prepared the stock of WSSV virus. The virus was lyophilized and then exposed to gamma and electron radiation and formalin with optimal dose for inactivated the virus. The shrimp divided to 25 groups and each group consist of six shrimp and the vaccine exposed to shrimp with injected and bathroom methods and the documented the result after 15 days. In vivo virus titration was performed in Penaeus indicus. Inactivation of WSSV was carried out by a gamma cell instrument Nordian, model 220 with dose rate: 4.8 Gy/sec and activity: 20469 Ci. The LD50 of live virus stock was calculated 10 5.4 / ml and the optimum dose of gamma radiation beam to inactivate WSSV was obtained 14-15 kGy. The LD50 of live virus stock was calculated 10 5.4 / ml and the optimum dose of electron beam to inactivate WSSV was obtained 12-13 kGy and and the virus inactivated by formalin 0.5% V/V during 10 minutes.The LD50 of live virus stock was calculated by Karber method 10 3.29 /ml and 10 5.35 /ml, respectively. The V. parahemolyticus was inactivated with 8 KG. The result showed all vaccine during 2 hours have a good effect to shrimp viruses with the 102.4 LD50 / 50 l titre. The result showed if the Gamma and electron vaccine increase to feed of shrimp the effect of vaccine is better than when used without feed. The end of experiment our study showed that the Gamma vaccine has the better effect to control WSSV during 2 hours with comparing the others

Identification of WSSV, TSV & IHHNV in wild and cultured shrimps and crustaceans in Khuzestan province - Abadan and investigation of its pathogenicity

According to the white spot disease during 2002 until 2005 and the damages caused by the disease to the farmers a new species L.vannamei were introduced to the farming region of Abadan Choebdeh from 2006. Hight mortality occurred in Khuzestan province farms in 2007 and 2008 again. Probably viral agent was transferred to native shrimps or other aquatic animals, therefore it is very important that presence of viruses in local aquatic animals and its vectors detected. Detection of WSSV, TSVand IHHNV in aquatic animal from Khuzestan costal region imphasis to wild shrimp and craps is the main objectives of this study. So samples were taken from 10 active farms twice a week and 100 postlarves from 3 active breeding center befor stocking. Samples for viral detection were studied by molecular and histopathology asseys. Results was shown presence of the White spot virus in cultured and wild shrimp and TSV infection in only two cultured shrimps but there was negative result for IHHN virus. To study the pathogenesis of white spot virus isolated from wild and farmed shrimps, exposure method was used. Results of challenge showed that WSSV can be transmited from diseased cultured and wild shrimp to cultured shrimp. Mean mortality percent in the treatment that fed with infected wild shrimp was 38.33±10.4 and treatment that fed with infected cultured shrimp 61.66±7.63 and in the positive control treatment was 87.5±3.5